Protein Alignment RhoGEF4 and arhgef1b

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: XP_021322373.1
• Gene: arhgef1b / 557983
• ZFINID: ZDB-GENE-060616-136
• Length: 1082
• Species: Danio rerio
• Alignment Length: 590
• Identity: 120/590 - (20%)
• Similarity: 209/590 - (35%)
• Gaps: 189/590 - (32%)

  Fly    13 FIHSPQTPLSFSR--ELRQ-VLQERNLLTAKSRRKVCSMLDSNNSSPIGSPNPDAGKRLGFRRQA 74
            |:.:||......|  ||.| .|..|.|.:::|..|:                   .|:...|::.
Zfish   482 FVPAPQQEEIEPRLLELEQDPLNWRELASSESLHKL-------------------SKKEAKRQEV 527

  Fly    75 IQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHTLLFGQIEMI---HNLNGEFLRELEANM 136
            |.|:..:|::::..|.:|...|.|||::|..:..:....:|..::.|   |.:..|.|::|....
Zfish   528 INELFVTEQAHVRMLSVLQTVFSRPLEKQEYMTANEVATIFSSLDDIIEMHYVFYENLKKLREED 592

  Fly   137 ENVAHA----------------FLKMAPFFKLYSVYAFDYRGALFIIQDLISKNPVFRKFLEQTE 185
            |.:...                |.|:...|..|..:|.:.      |:....|:|.|..|:.:.|
Zfish   593 EYIVKTISTPLLNRFSGSEGEWFQKLTARFCSYQSWALEQ------IKSRQKKDPRFNAFILEAE 651

  Fly   186 SRPEVQR-KLNSLMIVPIQRVPRYKLLLEQVLLYTSPADADYKL--LKESVKEIEATASHINTCV 247
            |:|:.:| :|..::.:.:||:.:|.||||.:...|..|....|:  ..||.::|   .:|:|..|
Zfish   652 SKPQCRRLQLKDIIPIEMQRLTKYPLLLENIAKNTEDATEKEKINQAAESCRKI---LNHVNEEV 713

  Fly   248 EEQE----ITQYLIHL-------QNSLVNRTPNIVKPSRRVIKEGVLQKITHKGTEIKRYCVLMS 301
            ::.|    :..|...|       .|.|.:....|...:|.::.||.|.....|...|..:|||:|
Zfish   714 KQMENLLILKDYQRRLDTSGLKPSNELYSEYKTIDLTNRTMLFEGPLTWRVTKEKAIDVHCVLLS 778

  Fly   302 DIFMYCK------MIK-ERAPNTVVENSLECCCIFPLKKCK-------------VYEMLPGN--- 343
            |:.:..:      ::| :...|..|:...:  .:.|:.|.:             :|.:...:
Zfish   779 DLLVLFQKQDDKMLLKCQSKSNIAVQEGKQ--MLSPIIKLESVFLREVATDPKALYVIFTWDSGA 841

  Fly   344 --FKLTCQSDGIIFGSGDVQLSRTWVGFIRDAIDLHVQCRKTLRKDSSKRTPIRKKDMKKFGADY 406
              ::|..||.|         ..:.|...|:.|:|       .|:|.|...|.:.:.
Zfish   842 QIYELVAQSVG---------ERKNWTEAIKSAVD-------ELKKASKSNTKLERN--------- 881

  Fly   407 VLSPNKRKCEYDTVFRNKNRSTDSEEETEDSACFSRKRKVASGILRAPNGNPMGKASSSSSSSVS 471
                                              ||...|       ||
Zfish   882 ----------------------------------SRAGSV-------PN---------------- 889

  Fly   472 MKRVAPPPPAPPPPPAVQMRHQPGRPGELATASKENRISKLYKKIAAGDK------VANVRGILK 530
              .:|.|...|..||.....:    .|||.    :||:.:..:||...|.      .||...:|.
Zfish   890 --NIAVPLYGPAHPPLSPTEN----GGELL----KNRVGRFSEKIRESDPPLIDYLAANGFDLLT 944

  Fly   531 RSNVP 535
            .|:.|
Zfish   945 HSHTP 949

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	46/191 (24%)
		PH-like	269..>311	CDD:302622	12/47 (26%)
		PH	277..374	CDD:278594	22/121 (18%)
arhgef1b	XP_021322373.1	RGS_p115RhoGEF	74..266	CDD:188709
		RhoGEF	524..710	CDD:238091	47/194 (24%)
		PH_p115RhoGEF	744..868	CDD:275429	26/141 (18%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.810