DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment RhoGEF4 and arhgef15b

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	XP_009304999.1	Gene:	arhgef15b / 555489	ZFINID:	ZDB-GENE-060810-103	Length:	937	Species:	Danio rerio

Alignment Length:	453	Identity:	102/453 - (22%)
Similarity:	168/453 - (37%)	Gaps:	123/453 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 TPRQKGYGNGFIHSPQTPLSFSRELRQVLQERNLLTAKSRRKVC-----SMLDSNNSSPIGSPNP 62
            |.|||        |.......|.||.|...| ..:..:.||.||     :..|...||.| :.:|
Zfish   410 TRRQK--------SVDLEAEMSDELYQFYTE-TYVHKEIRRTVCRNISKTSADFQMSSQI-TTSP 464

  Fly    63 DAGKR--------------LGFRRQAIQ--------------------EIISSEKSYLEQLELLM 93
            :.|::              |...:.|::                    |:|:||.|||..|.:|.
Zfish   465 EKGEKGPKSSESQSLKQNNLWREQSAVRDSGVLEKLSPEECKYQESMFEVITSEMSYLRSLRVLT 529

  Fly    94 NFFVR--PLKEQAIIDCSNHTLLFGQIEMIHNLNGEFLRELEANM-ENVA----------HAFLK 145
            ..||.  .|.|..||  .:...||..|..|..::..||::||..: ||:.          ||
Zfish   530 EHFVESSELNETLII--MDKKTLFSNIIRIQEVSERFLKDLEERLDENIIISDICDIIHYHA--- 589

  Fly   146 MAPFFKLYSVYAFDY-RGALF---IIQDLISKNPVFRKFLEQTESRPEVQR-KLNSLMIVPIQRV 205
             ...|..|    .|| |..::   ...:|:..|..|...:.:.:..|:.|| ...|.:::|.||:
Zfish   590 -QHHFPAY----IDYVRNQIYQEKTYSNLMQTNTQFATVITRLQESPKCQRLPFMSFLLLPFQRI 649

  Fly   206 PRYKLLLEQVLLYTSPADADYKLLKESVKEIEATASHINTCVEEQEITQYLIHLQNSL----VNR 266
            .|.|:|:|.:|..|:......:...:::..:.......||.|.:.:..:.||.:...|    :..
Zfish   650 TRIKMLIENILKRTAVGTDKEQTASKALDSVSKIIEECNTQVGKMKQMEELIQINGMLEFDKLKA 714

  Fly   267 TPNIVKPSRRVIKEGVLQKITHKGTEIKR--------YCVLMSDIFMYCKMIKERA--------- 314
            .| |:..:|.:.|:|.:.::...||.:..        |..|.:|:.:..  ||:.|
Zfish   715 VP-IISQTRYLEKQGEVLEVNKGGTFLGNLRPKFTPVYLFLFNDLLVIA--IKKSAERYVVLDHA 776

  Fly   315 ----------PNTVVENSLECCCIFPLKKCKVYEMLPGNFKLTCQSDGIIFGSGDVQLSRTWV 367
                      .|:.|.|...|.|:..|:..:..:| ....|...|||           |:.|:
Zfish   777 HRSLVQVQSYENSAVPNIEHCFCLTLLENHQGRQM-ERIMKAPSQSD-----------SQRWL 827

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	49/207 (24%)
		PH-like	269..>311	CDD:302622	9/49 (18%)
		PH	277..374	CDD:278594	23/118 (19%)
arhgef15b	XP_009304999.1	RhoGEF	511..688	CDD:279015	48/186 (26%)
		PH_ephexin	711..833	CDD:269929	26/132 (20%)
		PH	724..830	CDD:278594	23/118 (19%)
		SH3_ephexin1_like	852..906	CDD:212727
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.810