DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF4 and PLEKHG6

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	NP_001371533.1	Gene:	PLEKHG6 / 55200	HGNCID:	25562	Length:	806	Species:	Homo sapiens

Alignment Length:	694	Identity:	136/694 - (19%)
Similarity:	241/694 - (34%)	Gaps:	210/694 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 RQKGY-GNGFIHSPQTPLSFSRELRQVLQERNLLTAKSRRKVCSMLDSNNSSP-------IGSPN 61
            |..|: |.|.:|||        :|:::.:...|   :.|....||.......|       ||. .
Human    82 RHGGHVGAGLLHSP--------KLKELTKAHEL---EVRLHTFSMFGMPRLPPEDRRHWEIGE-G 134

  Fly    62 PDAG------------------KRLGFRRQAIQEIISSEKSYLEQLELLMNFFVRPL----KEQA 104
            .|:|                  :.|..:::|:.|::::|..|:.:|:::.:.....|    :...
Human   135 GDSGLTIEKSWRELVPGHKEMSQELCHQQEALWELLTTELIYVRKLKIMTDLLAAGLLNLQRVGL 199

  Fly   105 IIDCSNHTLLFGQIEMIHNLNGEF--------LRELEANME-----NVAHAFLKMAPFFKLYSVY 156
            :::.|..| |||.:..:...:..|        |.|..|:.:     .:...||.....|..|..|
Human   200 LMEVSAET-LFGNVPSLIRTHRSFWDEVLGPTLEETRASGQPLDPIGLQSGFLTFGQRFHPYVQY 263

  Fly   157 AFDYRGALFIIQDLISKNPVFRKFLE--QTESRPEV---------------QRKLNSLMIVPIQR 204
            ....:..:...::....||:|..|::  |..:.||.               ::.|..|:|.|.||
Human   264 CLRVKQTMAYAREQQETNPLFHAFVQAGQEGANPEPHPPSLQWCEKHKRSGRQMLCDLLIKPHQR 328

  Fly   205 VPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATASHINTCV---EEQE--------ITQY-LI 257
            :.:|.|||..| |..||.....:.|...::.:|:...|||..|   ||||        |..| ::
Human   329 ITKYPLLLHAV-LKRSPEARAQEALNAMIEAVESFLRHINGQVRQGEEQESLAAAAQRIGPYEVL 392

  Fly   258 HLQNSLVNR------TPNIVKP--------SRRVIKEGVLQKITHKGTEIKRYCVLMSDIFMYCK 308
            ...:..|.:      |.::..|        :|:::.||.::....:..::..|..|.||:.:..|
Human   393 EPPSDEVEKNLRPFSTLDLTSPMLGVASEHTRQLLLEGPVRVKEGREGKLDVYLFLFSDVLLVTK 457

  Fly   309 MIKERAPNTVVEN--SLECCCIFPLKKCKVYEMLPGNFKL------TCQSDGIIFGSGDVQLSRT 365
            ..::.....|:..  .||.....||:.       |.:|.|      .|.|..::...........
Human   458 PQRKADKAKVIRPPLMLEKLVCQPLRD-------PNSFLLIHLTEFQCVSSALLVHCPSPTDRAQ 515

  Fly   366 WVGFIRDA--------IDLHVQCRK---TLRKDSSKRTPIRKKDMKKFGADYVLSPNKRKCEYDT 419
            |:...:.|        .:.:||.::   ||.:|..:.:|..:.......... .|...|..|:.|
Human   516 WLEKTQQAQAALQKLKAEEYVQQKRELLTLYRDQDRESPSTRPSTPSLEGSQ-SSAEGRTPEFST 579

  Fly   420 VFRNKNRSTDSEEET----EDSACFSRKRKVASGILRAPNGNPMGKASSSSS------------- 467
            :..:...:.|::|:.    :|::        .||......|.|.|..|..|.
Human   580 IIPHLVVTEDTDEDAPLVPDDTS--------DSGYGTLIPGTPTGSRSPLSRLRQRALRRDPRLT 636

  Fly   468 -SSVSMKRVAPPPPAPPPPPAVQMRHQPGRPGELATASKENRISKLYKKIAAGDKVANVRGILKR 531
             |::.::.: |..|.||.|.|.|.|..|..|                            .||||.
Human   637 FSTLELRDI-PLRPHPPDPQAPQRRSAPELP----------------------------EGILKG 672

  Fly   532 SNVPAPNNDHDPSYGFASRYSDSKSYFRAHNVDNTIPTFVKRED 575
            .::|    ..||                        ||:.:.||
Human   673 GSLP----QEDP------------------------PTWSEEED 688

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	44/203 (22%)
		PH-like	269..>311	CDD:302622	9/49 (18%)
		PH	277..374	CDD:278594	19/112 (17%)
PLEKHG6	NP_001371533.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	63..91		3/8 (38%)
		RhoGEF	165..367	CDD:395496	44/203 (22%)
		PH_PLEKHG5_G6	424..522	CDD:270064	19/104 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	545..693		39/210 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	706..746
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	764..806
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810