DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF4 and Ngef

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	NP_001104784.1	Gene:	Ngef / 53972	MGIID:	1858414	Length:	710	Species:	Mus musculus

Alignment Length:	471	Identity:	99/471 - (21%)
Similarity:	190/471 - (40%)	Gaps:	98/471 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly    32 QERNLLTAKSRR---KVCSMLDSNNSSPIGSPNPDAGK------------RLGFRRQAIQEIISS 81
            :|..|.:...||   ::|.:.:.::...:....|:...            ||   ::|:.|:::|
Mouse   223 EEEELASPPERRALPQICLLSNPHSRFNLWQDLPEIQSSGVLDILQPEEIRL---QEAMFELVTS 284

  Fly    82 EKSYLEQLELLMNFFVRPLKEQAIIDCSNHTLLFGQIEMIHNLNGEFLRELEANM-ENVAHA--- 142
            |.||.:.|.||::.|:...:.:.|:..|...:||..:..:..::..||.|||..| ||:..:
Mouse   285 EASYYKSLNLLVSHFMENERLKKILHPSEAHILFSNVLDVMAVSERFLLELEHRMEENIVISDVC 349

  Fly   143 ---FLKMAPFFKLYSVYAFDYRGALFIIQDLISKNPVFRKFLEQTESRPEVQ-RKLNSLMIVPIQ 203
               :...|..|.:|..|..:........:.|:.:...||:.:.|.|..|:.: ...:|.:|:|.|
Mouse   350 DIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCKGLPFSSFLILPFQ 414

  Fly   204 RVPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATASHINTCVEEQEITQYLIHLQNSL---VN 265
            |:.|.|||::.:|..............::.||:|......|..|.:...|:.:|.:|..:   :.
Mouse   415 RITRLKLLVQNILKRVEERSEREGTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIK 479

  Fly   266 RTPNIVKPSRRVIKEGVLQKI----THKGTEIKR-----YCVLMSDIFMYCKMIKERAPNTVVEN 321
            ..| |:..||.::|:|.||::    |.:....|:     |..|.:|:.:.|:.|
Mouse   480 SVP-IISHSRWLLKQGELQQMSGPKTSRTLRTKKLFREIYLFLFNDLLVICRQI----------- 532

  Fly   322 SLECCCIFPLKKCKVYEMLP-GNFKLTCQSDGIIFGSGDVQLSRTWVGFIRDAIDLHVQCRKTLR 385
                    |..|.:|::..| |..::....|     .|....:...:..:.:|.|...   ..:.
Mouse   533 --------PGDKYQVFDSAPRGLLRVEELED-----QGQTLANVFILRLLENADDREA---TYML 581

  Fly   386 KDSSKRTPIRKKDMKKFGADYVLSPNKR----------------KCEYDTVFRNKNRST------ 428
            |.||      :.:||::...  |:||:|                :|.:..|.:..:..|
Mouse   582 KASS------QSEMKRWMTS--LAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADI 638

  Fly   429 -DSEEETEDSACFSRK 443
             :..|:|||...|..:
Mouse   639 LNILEKTEDGWIFGER 654

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	45/177 (25%)
		PH-like	269..>311	CDD:302622	13/50 (26%)
		PH	277..374	CDD:278594	18/106 (17%)
Ngef	NP_001104784.1	Regulatory region, modulates activity toward RHOA, RAC1 and CDC42	1..272		6/48 (13%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..146
		ELH	3..>156	CDD:145463
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	192..234		2/10 (20%)
		RhoGEF	277..456	CDD:279015	45/178 (25%)
		PH_ephexin	477..603	CDD:269929	33/161 (20%)
		PH	490..596	CDD:278594	25/140 (18%)
		SH3_ephexin1	616..670	CDD:212872	8/39 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	688..710
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810