Protein Alignment RhoGEF4 and ARHGEF3

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: NP_001122087.1
• Gene: ARHGEF3 / 50650
• HGNCID: 683
• Length: 558
• Species: Homo sapiens
• Alignment Length: 494
• Identity: 100/494 - (20%)
• Similarity: 190/494 - (38%)
• Gaps: 108/494 - (21%)

  Fly    19 TPLSFSRELRQVLQERNLLTAKSRRKVCSMLD-SNNSSPIGSPNPDA----------------GK 66
            |||   :...|.||......::||..:.:... |.|::|..:...|:                .|
Human    90 TPL---KRFSQTLQRSISFRSESRPDILAPRPWSRNAAPSSTKRRDSKLWSETFDVCVNQMLTSK 151

  Fly    67 RLGFRRQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHTLLFGQIEMIHNLNGEFLRE 131
            .:. |::||.|:...|:..:|.|:|....:..|:.:.:|:.......:||.::.:..|:.|.|.:
Human   152 EIK-RQEAIFELSQGEEDLIEDLKLAKKAYHDPMLKLSIMTEQELNQIFGTLDSLIPLHEELLSQ 215

  Fly   132 L------EANMENVAHAFLKMAPFFKLYSVYAFDYRGALFIIQDLISKNPVFRKFLEQTESRPEV 190
            |      :.:.|:|....:...|....|..|..:...|..:: |...::...:.||::....| .
Human   216 LRDVRKPDGSTEHVGPILVGWLPCLSSYDSYCSNQVAAKALL-DHKKQDHRVQDFLQRCLESP-F 278

  Fly   191 QRKLN--SLMIVPIQRVPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATASHINTCVEEQEIT 253
            .|||:  :.:.:|..|:.:|.|||.::|.:|...:.|.:.|:|::..|:...:.|||...|.|..
Human   279 SRKLDLWNFLDIPRSRLVKYPLLLREILRHTPNDNPDQQHLEEAINIIQGIVAEINTKTGESECR 343

  Fly   254 QY---LIHLQNSLVNRTPNIVKPSRRVIKEGVLQKITHKGTEIKRYCVLMSDIFMYCKMIKERAP 315
            .|   |::|:.   .:..:::..||.:...|.|:  .::|  :|.:..|..::.:..:
Human   344 YYKERLLYLEE---GQKDSLIDSSRVLCCHGELK--NNRG--VKLHVFLFQEVLVITR------- 394

  Fly   316 NTVVENSLECCCIF----PLKKCKVYEMLPG------------------------NFKLTCQSDG 352
             .|..|...|..::    |:|...:.::..|                        :||...||..
Human   395 -AVTHNEQLCYQLYRQPIPVKDLLLEDLQDGEVRLGGSLRGAFSNNERIKNFFRVSFKNGSQSQT 458

  Fly   353 IIFGSGDVQLSRTWVGFIRDAIDLHVQCR----------------KTLRKDSSKRTPIRKKDMKK 401
            ....:.|....:.|:..||.|.:. |.|.                .|..::....|.:.:.|.
Human   459 HSLQANDTFNKQQWLNCIRQAKET-VLCAAGQAGVLDSEGSFLNPTTGSRELQGETKLEQMDQ-- 520

  Fly   402 FGADYVLSPNKRKCEYDTVFRNKNRSTDSEE-ETEDSAC 439
                   |.::..|..||    ...|.|.|. |..||:|
Human   521 -------SDSESDCSMDT----SEVSLDCERMEQTDSSC 548

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	40/177 (23%)
		PH-like	269..>311	CDD:302622	7/41 (17%)
		PH	277..374	CDD:278594	19/124 (15%)
ARHGEF3	NP_001122087.1	RhoGEF	158..334	CDD:395496	40/177 (23%)
		PH_RhoGEF3_XPLN	348..480	CDD:269976	23/146 (16%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810