DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment RhoGEF4 and Fgd6

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	NP_001131117.1	Gene:	Fgd6 / 500824	RGDID:	1565609	Length:	1406	Species:	Rattus norvegicus

Alignment Length:	567	Identity:	126/567 - (22%)
Similarity:	219/567 - (38%)	Gaps:	153/567 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly    50 DSNNSSPIGSPNP-------DAGKRLGFRRQAIQEIISSEKSYLEQLELL-MNF----------F 96
            |.::.|..|.|:|       |||.:......| :||:||||.:::.|:|| ::|          .
  Rat   822 DVSSESSKGEPDPLEDKQDEDAGMKSKVHHIA-KEIMSSEKVFVDVLKLLHIDFRGAVAHASRQL 885

  Fly    97 VRPLKEQAIIDCSNHTLLFGQIEMIHNLNGEFLRELE------ANMENVAHAFLKMAPFFKLYSV 155
            .:|:.|..|:   |..|.:  :..::.||.:.|:|||      |..:.:|..|:|..|:.|:||:
  Rat   886 GKPVIEDRIL---NQILYY--LPQLYELNRDLLKELEERMLSWAEQQRIADIFVKKGPYLKMYSM 945

  Fly   156 YAFDYRGALFIIQDLISKNPVFRKFLEQTESRPE-VQRKLNSLMIVPIQRVPRYKLLLEQVLLYT 219
            |..::...:.::.:...||..|...:.:.|..|. ....|...::.|:||:|:|:|||...|...
  Rat   946 YIKEFDKNIALLDEQCKKNSGFATVVREFEMSPRCANLALKHYLLKPVQRIPQYRLLLTDYLKNL 1010

  Fly   220 SPADADYKLLKESVKEIEATASHINTCVEEQEITQYLIHLQNSLVNRTPNIVKPSRRVIKEGVLQ 284
            .....|::..::::..:...|:|.|..:::.:..|.|:.:|.||... ..||:|.|..:|||:|.
  Rat  1011 LEDSVDHRDTQDALAVVIEVANHANDTMKQGDNFQKLMQIQYSLSGH-HEIVQPGRVFLKEGILM 1074

  Fly   285 KITHKGTEIKRYCVLMSDIFMYCK-----MIKERAPNTVVENSLECCCIFPLKKCKV----YEML 340
            |::.|..: .|...|.:|..:|..     |.|       :.|.|.      |...||    .|..
  Rat  1075 KLSRKVMQ-PRMIFLFNDALLYTTPMQSGMYK-------LNNMLS------LAGMKVRKPTQEAY 1125

  Fly   341 PGNFKLTCQSDGIIFGSGDVQLSRTWVGFIRDAIDLHVQCR------KTLRKDSSKR---TPIRK 396
            ....|:.......|..:........|:..|..||:.:.:.|      ::|.:||.::   :|:..
  Rat  1126 QNELKIESVERSFILSASSASERDDWLEAISRAIEEYAKKRITFCPSRSLDEDSERKEEVSPLGA 1190

  Fly   397 K----------------------------------------DMKKFGADYVLSPNKRKCEYDTVF 421
            |                                        ...|.|.||:.....|.||
  Rat  1191 KAPIWIPDTRATMCMICTSEFTLTWRRHHCRACGKIVCQACSSNKCGLDYLKGQPARVCE----- 1250

  Fly   422 RNKNRSTDSEEETEDSACFSRKRKVASGILRAPN-GNPMGKASSSSSSSVSMKRVAPPPPAPPPP 485
                            .||...:|:...:  :|. |:| |...|.||:..|:....|
  Rat  1251 ----------------LCFQELQKLDHQL--SPRIGSP-GNHKSPSSALSSVLHSIP-------- 1288

  Fly   486 PAVQMRHQPGRPGELATASKENRISKLYKKIAAGDKVANVRGILKRS 532
                    .||        |:.:|....|:::|..:.:.:.|.|.||
  Rat  1289 --------SGR--------KQKKIPAALKEVSANTEDSTMSGYLYRS 1319

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	49/187 (26%)
		PH-like	269..>311	CDD:302622	15/46 (33%)
		PH	277..374	CDD:278594	22/105 (21%)
Fgd6	NP_001131117.1	RhoGEF	852..1036	CDD:279015	49/189 (26%)
		PH1_FGD6	1054..1176	CDD:275436	30/136 (22%)
		PH	1069..1160	CDD:278594	23/104 (22%)
		FYVE_FGD6	1193..1253	CDD:277282	7/80 (9%)
		PH	1311..1402	CDD:278594	4/9 (44%)
		PH2_FGD5_FGD6	1312..1400	CDD:270057	4/8 (50%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X316
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.910