Protein Alignment RhoGEF4 and Fgd6

DIOPT Version :10

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: NP_001131117.1
• Gene: Fgd6 / 500824
• RGDID: 1565609
• Length: 1406
• Species: Rattus norvegicus
• Alignment Length: 567
• Identity: 126/567 - (22%)
• Similarity: 219/567 - (38%)
• Gaps: 153/567 - (26%)

  Fly    50 DSNNSSPIGSPNP-------DAGKRLGFRRQAIQEIISSEKSYLEQLELL-MNF----------F 96
            |.::.|..|.|:|       |||.:......| :||:||||.:::.|:|| ::|          .
  Rat   822 DVSSESSKGEPDPLEDKQDEDAGMKSKVHHIA-KEIMSSEKVFVDVLKLLHIDFRGAVAHASRQL 885

  Fly    97 VRPLKEQAIIDCSNHTLLFGQIEMIHNLNGEFLRELE------ANMENVAHAFLKMAPFFKLYSV 155
            .:|:.|..|:   |..|.:  :..::.||.:.|:|||      |..:.:|..|:|..|:.|:||:
  Rat   886 GKPVIEDRIL---NQILYY--LPQLYELNRDLLKELEERMLSWAEQQRIADIFVKKGPYLKMYSM 945

  Fly   156 YAFDYRGALFIIQDLISKNPVFRKFLEQTESRPE-VQRKLNSLMIVPIQRVPRYKLLLEQVLLYT 219
            |..::...:.::.:...||..|...:.:.|..|. ....|...::.|:||:|:|:|||...|...
  Rat   946 YIKEFDKNIALLDEQCKKNSGFATVVREFEMSPRCANLALKHYLLKPVQRIPQYRLLLTDYLKNL 1010

  Fly   220 SPADADYKLLKESVKEIEATASHINTCVEEQEITQYLIHLQNSLVNRTPNIVKPSRRVIKEGVLQ 284
            .....|::..::::..:...|:|.|..:::.:..|.|:.:|.||... ..||:|.|..:|||:|.
  Rat  1011 LEDSVDHRDTQDALAVVIEVANHANDTMKQGDNFQKLMQIQYSLSGH-HEIVQPGRVFLKEGILM 1074

  Fly   285 KITHKGTEIKRYCVLMSDIFMYCK-----MIKERAPNTVVENSLECCCIFPLKKCKV----YEML 340
            |::.|..: .|...|.:|..:|..     |.|       :.|.|.      |...||    .|..
  Rat  1075 KLSRKVMQ-PRMIFLFNDALLYTTPMQSGMYK-------LNNMLS------LAGMKVRKPTQEAY 1125

  Fly   341 PGNFKLTCQSDGIIFGSGDVQLSRTWVGFIRDAIDLHVQCR------KTLRKDSSKR---TPIRK 396
            ....|:.......|..:........|:..|..||:.:.:.|      ::|.:||.::   :|:..
  Rat  1126 QNELKIESVERSFILSASSASERDDWLEAISRAIEEYAKKRITFCPSRSLDEDSERKEEVSPLGA 1190

  Fly   397 K----------------------------------------DMKKFGADYVLSPNKRKCEYDTVF 421
            |                                        ...|.|.||:.....|.||
  Rat  1191 KAPIWIPDTRATMCMICTSEFTLTWRRHHCRACGKIVCQACSSNKCGLDYLKGQPARVCE----- 1250

  Fly   422 RNKNRSTDSEEETEDSACFSRKRKVASGILRAPN-GNPMGKASSSSSSSVSMKRVAPPPPAPPPP 485
                            .||...:|:...:  :|. |:| |...|.||:..|:....|
  Rat  1251 ----------------LCFQELQKLDHQL--SPRIGSP-GNHKSPSSALSSVLHSIP-------- 1288

  Fly   486 PAVQMRHQPGRPGELATASKENRISKLYKKIAAGDKVANVRGILKRS 532
                    .||        |:.:|....|:::|..:.:.:.|.|.||
  Rat  1289 --------SGR--------KQKKIPAALKEVSANTEDSTMSGYLYRS 1319

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:459876	49/187 (26%)
		PH-like	269..>311	CDD:473070	15/46 (33%)
		PH	277..374	CDD:459697	22/105 (21%)
Fgd6	NP_001131117.1	RhoGEF	852..1035	CDD:459876	49/188 (26%)
		PH1_FGD6	1054..1176	CDD:275436	30/136 (22%)
		FYVE_FGD6	1193..1253	CDD:277282	7/80 (9%)
		PH2_FGD5_FGD6	1312..1400	CDD:270057	4/8 (50%)