Protein Alignment RhoGEF4 and net1

DIOPT Version :10

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: XP_021330686.1
• Gene: net1 / 493607
• ZFINID: ZDB-GENE-041121-18
• Length: 605
• Species: Danio rerio
• Alignment Length: 494
• Identity: 98/494 - (19%)
• Similarity: 193/494 - (39%)
• Gaps: 97/494 - (19%)

  Fly    42 RRKVCSML-----DSNNSSPIGSPNPDAGKRLGF------RRQAIQEIISSEKSYLEQLELLMNF 95
            ::|.||..     ....:|.:.|...|..::..|      |::||.|:...|:..:|.|:|....
Zfish   134 QQKPCSKAAAPTPPKRRNSTLWSETLDVHQKGTFSTKEIKRQEAIFELSRGEQDLIEDLKLARKA 198

  Fly    96 FVRPLKEQAIIDCSNHTLLFGQIEMIHNLNGEFLREL------EANMENVAHAFLKMAPFFKLYS 154
            :..|:.:.:|:.....|.:||.::....|:.:.|.:|      :..:..:....:...|....|.
Zfish   199 YHDPMLKLSIMSEEELTAIFGDLDAYIPLHEDLLDQLAKATGTDGTVRQIGKIVVDWLPRLNAYR 263

  Fly   155 VYAFDYRGALFIIQDLISKNPVFRKFLEQTESRPEVQRKLN--SLMIVPIQRVPRYKLLLEQVLL 217
            .|..:...|..:: |...::|..:.||::....| ..|||:  |.:.:|..|:.:|.|||:::|.
Zfish   264 AYCSNQLAAKALL-DQKKQDPRVQDFLQRCLESP-FSRKLDLWSFLDIPRSRLVKYPLLLKEILR 326

  Fly   218 YTSPADADYKLLKESVKEIEATASHINTCVEEQEITQYLIHLQNSLVNRTPNIVKPSRRVIKEGV 282
            :|.|...|...|::::..|:|..:.||....|.|...|:..|:.....:....::..:.::..|.
Zfish   327 HTPPDHPDKGSLEQAISVIQAVLADINMKKGESECQYYIDKLEYLEDKQKDPRIEQCKSLLCHGE 391

  Fly   283 LQKITHKGTEIKRYCVLMSDIFMYCKMIKERAPNTVVENSLECCCIF----PLKKCKVYEMLPGN 343
            |:  ...||::        .:|::.:::....|  |..|...|..::    |::...:.::..|:
Zfish   392 LR--NKSGTKL--------HVFLFTEVLILTRP--VTRNDRHCFQVYRQPIPVQDLVLEDLQDGD 444

  Fly   344 ------------------------FKLTCQSDGIIFGSGDVQLSRTWVGFIRDAIDLHVQCRKTL 384
                                    |:...|.........||...:.|:..:|:|  :..|...||
Zfish   445 VRMGGSFRGAFSNADKAKNIFRVRFQDASQGQSHTLQVNDVFHKQQWLNCLRNA--MSTQKDLTL 507

  Fly   385 RKDSSKRTPIRKKDMKKFGADYVLSPNKRKCEYDTVFRNKNRSTDSEEETEDSACFSRKRKVASG 449
            .::....||......|:                    |:.:.||..:.:..|..|   .|..||
Zfish   508 SENEPLTTPATDVCAKR--------------------RSSSVSTVIQMDEMDENC---PRAAAS- 548

  Fly   450 ILRAPNG-------NPMGKASSSSSSSVSMKRVAPPPPA 481
               ||:.       :|...|:|:.|||.|...::.|.|:
Zfish   549 ---APSSPSSEEPPSPTPSATSTLSSSSSSSSISAPIPS 584

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:459876	41/177 (23%)
		PH-like	269..>311	CDD:473070	5/41 (12%)
		PH	277..374	CDD:459697	17/124 (14%)
net1	XP_021330686.1	RhoGEF	177..353	CDD:214619	41/177 (23%)
		PH-like	367..500	CDD:473070	19/146 (13%)