DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment RhoGEF4 and arhgef18a

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	NP_999983.1	Gene:	arhgef18a / 407987	ZFINID:	ZDB-GENE-040516-14	Length:	940	Species:	Danio rerio

Alignment Length:	659	Identity:	125/659 - (18%)
Similarity:	242/659 - (36%)	Gaps:	166/659 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly    63 DAGKRLGFRRQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHTLLFGQIEMIHNLNGE 127
            ||.|    |:..|.|:|.:|.:::..|::::|.::|.|:....:|.:....||.|:|.:..::..
Zfish    67 DAVK----RQDVIHELIQTEINHVRTLKIVLNVYIRELRNTLQMDETRLERLFPQVENLLEVHQF 127

  Fly   128 FLRELEA----NME----------NVAHAFLKM------APFFKLYSVYAFDYRGALFIIQDLIS 172
            ||..|:.    :||          |:....:..      :....||.::...:..|:...:||:.
Zfish   128 FLDNLKLRRLDSMEPGSSQNYCIHNLGDILITQFSGEIGSRLRYLYGMFCSRHTDAVNFYKDLMQ 192

  Fly   173 KNPVFRKFLEQTESRPEVQRKLN--SLMIVPIQRVPRYKLLLEQVLLYTSPADADYKLLKESVKE 235
            .|..|:..:.:. .:..:.|:|.  .::::..||:.:|.:|.|:|:.||.....:...|.:....
Zfish   193 NNKKFQNLIRKI-CQLSIVRRLGIPEIILLITQRITKYPVLFERVIKYTEDESEEQMDLIKGPDF 256

  Fly   236 IEATASHINTCVEEQEITQYLIHLQNSL------------VNRTPNIVKPSRRVIKEGVLQ-KIT 287
            ::.|.:.:|..|:|.|....|..|.:.|            |.|..::::..|:::.||:|. ::|
Zfish   257 LKDTIAQVNAHVDEAEKEARLRELSSKLEPKSQVKMTSGRVFRREDMLQGKRKLLHEGMLNWRVT 321

  Fly   288 H------KGTEIKRYCVLMSDIFMYC-----------------------KMIKERAPNT----VV 319
            :      |.|::  ..:|:||:.:..                       .:::|.|.|.    ::
Zfish   322 NNKGKGDKHTDV--IVLLLSDVLLLLLEKDSRLTFASLDGKPAVISLLKLIVREAAHNEKAMFLI 384

  Fly   320 ENSLECCCIFPLKKCKVYEMLPGNFKLTCQSDGIIFGSGDVQLSRTWVGFIRDAIDLHVQCRKTL 384
            ..||:.    |    ::||                |.:...:...||...|..|    |:|...:
Zfish   385 SASLDT----P----EMYE----------------FHTSSAEERNTWTNKIWKA----VECCPDV 421

  Fly   385 RKDSSKRTPIRKKDMKKFGADYVLSPNKRKCEYDTVFRNKNRSTDSEEETEDSACFSRKRKVASG 449
            :::..:.  :.||         :....::.|.:|        ::..:..||....||   .:|:.
Zfish   422 KEEEPEE--LSKK---------IWEFQRKLCVHD--------ASIEQHLTEKLKLFS---SIAAS 464

  Fly   450 ILRAPNGNPMGKASSSSSSSVSMKRVAPPPPAPPPPPAVQMRHQPGRPGE--LATASKE-NRISK 511
            :        .|.:.:|:|....::..|..|                ..||  |..|.|: ..:..
Zfish   465 V--------TGVSDTSTSERHPLRGNAAEP----------------LQGERLLTGALKDVENLQN 505

  Fly   512 LYKKIAAGDKVANVRG---ILKRSNVPA--PNNDHD-PSYGFASRYSDSKSYFRAHNVD---NTI 567
            |......|......|.   :||:.:...  .||.|. |..||.|.........:..|.|   ...
Zfish   506 LLVMCEQGPTPFTSRATDLVLKKPHAVTGLENNTHMLPENGFESSMESMGDRIQVANTDLDPQLR 570

  Fly   568 PTFVKREDLFNGDNMFPVHLKSHEEICTPPQKKRVKFDDTLDAVSPVL---VEKPFAFSSESSSA 629
            ...:......:.|....||....|...|..|..|..:  :|.:|...|   ::...|...|..|.
Zfish   571 ELHLSENQELSADENLSVHFPKAEFYSTVSQLSRKLY--SLQSVVQQLESHIKVQHATIEELKSR 633

  Fly   630 LKPTSLRER 638
            .:..||.|:
Zfish   634 PRGNSLLEQ 642

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	38/191 (20%)
		PH-like	269..>311	CDD:302622	10/71 (14%)
		PH	277..374	CDD:278594	21/130 (16%)
arhgef18a	NP_999983.1	RhoGEF	74..266	CDD:279015	38/192 (20%)
		PH-like	307..427	CDD:302622	25/149 (17%)
		DUF4200	650..747	CDD:290574
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.810