DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF4 and RhoGEF2

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	NP_477317.1	Gene:	RhoGEF2 / 36915	FlyBaseID:	FBgn0023172	Length:	2559	Species:	Drosophila melanogaster

Alignment Length:	673	Identity:	135/673 - (20%)
Similarity:	251/673 - (37%)	Gaps:	211/673 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly    63 DAGKRLGFRRQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHTLLF-GQIEMIHNLNG 126
            ||.|:   |::.|.||..:|::::..|:||...|..||.|..::...:..||| ..:..:..::|
  Fly  1534 DAEKK---RQEIINEIYQTERNHVRTLKLLDRLFFLPLYESGLLSQDHLLLLFPPALLSLREIHG 1595

  Fly   127 EFLRELEANMENVAHAFL------KMAPFF---------KLYSVYAFDYRGALFIIQDLISKNPV 176
            .|.:.|:  ...:.|..:      .:|..|         :..:.:....:.||..:::..:|:.:
  Fly  1596 AFEQSLK--QRRIEHNHVVNTIGDLLADMFDGQSGVVLCEFAAQFCARQQIALEALKEKRNKDEM 1658

  Fly   177 FRKFLEQTESRPEVQR-KLNSLMIVPIQRVPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATA 240
            .:|.|:::||....:| :|..|:...:||:.:|.||.|.:...|      .:||.|:..|.||
  Fly  1659 LQKLLKKSESHKACRRLELKDLLPTVLQRLTKYPLLFENLYKVT------VRLLPENTTEAEA-- 1715

  Fly   241 SHINTCVEEQEITQYLIHLQNSLVNRTPNIVKPSRRVIKEGVLQKI-----THKGTEIKRYCVLM 300
                                   :.|.   |:.|:|::.| |.|.:     .||...|:|    .
  Fly  1716 -----------------------IQRA---VESSKRILVE-VNQAVKTAEDAHKLQNIQR----K 1749

  Fly   301 SDIFMYCKMIKERAPNTVVENSLECCCIFPLKKCKVYEMLPGNFKL----TCQSDGIIFGSGDVQ 361
            .|...|.|  :|.....:.::.|            :::   ||..:    :.|..|::|.:..|.
  Fly  1750 LDRSSYDK--EEFKKLDLTQHHL------------IHD---GNLTIKKNPSVQLHGLLFENMIVL 1797

  Fly   362 LSR-----------TWVGFIRDAID--LHVQCRKTLRKDSSKRTPIRKKDMKKFGADYVLSPNKR 413
            |::           |.:......:.  :.:.....:|::::.:.......||......:.:|:..
  Fly  1798 LTKQDDKYYLKNLHTPLSITNKPVSPIMSIDADTLIRQEAADKNSFFLIKMKTSQMLELRAPSSS 1862

  Fly   414 KCEYDTVF-------------RNKNRSTDSEEETEDSACFSRKRKVASGILRAPNGNPMGKASSS 465
            :|:  |.|             |:||.|::.:....|.|           :...|:.|      :.
  Fly  1863 ECK--TWFKHFSDVAARQSKNRSKNASSNHDTSISDPA-----------LAAIPHSN------TK 1908

  Fly   466 SSSSVSMKRVAP---------PPPAP-------PPPPA-----------VQMRHQPGRPGELATA 503
            .|..:|...|.|         .|.||       .|.||           ||:|:    |...|||
  Fly  1909 ESLELSTDTVQPLAATATLTTTPLAPMLPIATVTPAPATNNSNVSSLTGVQLRN----PQRDATA 1969

  Fly   504 SK--------------ENRISK----------LYKKIAAGDKVANVRGILKRSNVP-------AP 537
            |:              :|.:::          :.|..|.|.:..:|  .|:.|...       :.
  Fly  1970 SESDADYVNTPKPRSSQNEVNRTMSIRSTGEPIQKYSANGTEANDV--TLRHSQSTRESVRPGST 2032

  Fly   538 NNDHDPSYGFASRYS--DSKSYFRAHNVDNTIPTFVKREDLFNGD----NMFPVHLKSHEEICTP 596
            ..:.:.:||.....|  ||.|...::|.:|| .|.:.:..|.:..    ::.|.|  :.|.:.||
  Fly  2033 GEERNSTYGMVGGNSKRDSASIVCSNNSNNT-RTLLMQSPLVDPTAIQVSISPAH--TAEPVLTP 2094

  Fly   597 PQKKRVKFDDTLDA-VSPVLVEK 618
            .:|.|     .||| :...|:||
  Fly  2095 GEKLR-----RLDASIRNDLLEK 2112

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	43/186 (23%)
		PH-like	269..>311	CDD:302622	13/46 (28%)
		PH	277..374	CDD:278594	20/116 (17%)
RhoGEF2	NP_477317.1	PDZ	260..334	CDD:214570
		RGS_GEF_like	934..1055	CDD:188710
		C1_1	1151..1202	CDD:278556
		RhoGEF	1539..1731	CDD:238091	49/228 (21%)
		PH_RhoGEF	1770..1875	CDD:275411	16/121 (13%)
		DUF2413	2242..>2378	CDD:287302
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm46934
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	3	2.810