Protein Alignment RhoGEF4 and Arhgef12

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: NP_001013264.2
• Gene: Arhgef12 / 367072
• RGDID: 1305111
• Length: 1545
• Species: Rattus norvegicus
• Alignment Length: 583
• Identity: 115/583 - (19%)
• Similarity: 205/583 - (35%)
• Gaps: 185/583 - (31%)

  Fly    71 RRQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHTLLFGQIEMIHNLN---------- 125
            |::.|.|:..:|::::..|::|...|.:.:..:.|:..|....:|..:|.|..|:
  Rat   788 RQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHVGLNEQMKAV 852

  Fly   126 ------------GE-----FLRELEANMENVAHAFLKMAPFFKLYSVYAFDYRGALFIIQDLISK 173
                        ||     |....|..:::.|..|....||             ||.:|:....|
  Rat   853 RKRNETSVIDHIGEDLLLWFSGPGEEKLKHAAATFCSNQPF-------------ALEMIKSRQKK 904

  Fly   174 NPVFRKFLEQTESRPEVQR-KLNSLMIVPIQRVPRYKLLLEQVLLYTS-PADADYKLLKESVKEI 236
            :..|:.|::..||.|..:| :|..::...:||:.:|.|||:.:..||. |.:.:  .:|::..:.
  Rat   905 DSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPPERE--KVKKAADQC 967

  Fly   237 EATASHINTCVEEQEITQYLIHLQNSL------------VNRTPNIVKPSRRVIKEGVLQKITHK 289
            ....:::|..|.|.|..|.|...|..|            |:...|:....|::|.||.|....::
  Rat   968 RQILNYVNQAVREAENKQRLEDYQRRLDTSSLKLSEYPNVDELRNLDLTKRKMIHEGPLVWKVNR 1032

  Fly   290 GTEIKRYCVLMSDIFMYCKMIKERAPNTVVENSLECCCIFPLKKCKVYEMLPGNFKLTCQSDGII 354
            ...|..|.:|:.||.:..:...:|                              ..|.|.|. |:
  Rat  1033 DKSIDLYTLLLEDILVLLQKQDDR------------------------------LVLRCHSK-IL 1066

  Fly   355 FGSGDVQLSRTWVGFIRDAIDLHVQCRKTLRKDSSKRT--PIRKKD---MKKFGAD----YVLSP 410
            ..:.|                             ||.|  |:.|..   :::...|    :|:|.
  Rat  1067 ASTAD-----------------------------SKHTFSPVIKLSTVLVRQVATDNKALFVISM 1102

  Fly   411 NKRKCE-YDTVFRNKNRSTDSEEET-EDSACFSRKRKVASGILRAPNGNPMGKASSSSSSSVSMK 473
            :....: |:.|     ..|.||:.. :|..|     ::|:.:          |..|:.
  Rat  1103 SDNGAQIYELV-----AQTVSEKTVWQDLIC-----RMAASV----------KEQSTK------- 1140

  Fly   474 RVAPPPPAPPPPPAVQMRHQPGRPGELATASKENRISKLYKKIAAGDKVANVRGILKRSNVPAPN 538
                |.|.|.|||. :..:....|.:|.....:..::.|    .:.|:|..:...| .|:.|..:
  Rat  1141 ----PIPLPQPPPC-EGDNDEEEPAKLKVEHHDLSVAGL----QSPDRVLGLESPL-ISSKPQSH 1195

  Fly   539 NDHDPSYGFASRYSDSKSYFRAHNVDNTIPTFVKRE----DLFNGDNMFPV-----HLKSHEE 592
            :.:.|          .||  .|.::..|...|.|.:    ....||..:||     ||...||
  Rat  1196 SMNTP----------GKS--EAEHLFVTATQFAKEQHANGTFKEGDGGYPVTISDPHLPVSEE 1246

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	41/198 (21%)
		PH-like	269..>311	CDD:302622	11/41 (27%)
		PH	277..374	CDD:278594	15/96 (16%)
Arhgef12	NP_001013264.2	None

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810