Protein Alignment RhoGEF4 and Arhgef19

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: NP_001102162.1
• Gene: Arhgef19 / 362648
• RGDID: 1304766
• Length: 888
• Species: Rattus norvegicus
• Alignment Length: 557
• Identity: 116/557 - (20%)
• Similarity: 207/557 - (37%)
• Gaps: 125/557 - (22%)

  Fly    11 NGFIHSPQTPLSFSRELRQVLQER-------------------NLLTAKSRRKVCSMLDSNNSSP 56
            |..::...:.::.:||||:..:|.                   ||..:.|.|...|...|..|
  Rat   377 NSVLYQEYSDVASARELRRQQREEEGPGDGAEGAEEGPGPPRANLSPSSSFRAQRSTRGSTFS-- 439

  Fly    57 IGSPNPDAG-----KRLGFR----RQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHT 112
            :....||..     ..|..|    ::|..|:|:||.||:..|.:.:..|:..::....:...:..
  Rat   440 LWQDIPDVRGSGVLATLSLRDCKLQEAKFELITSEASYIHSLSVAVGHFLGSVELSECLGTQDKQ 504

  Fly   113 LLFGQIEMIHNLNGEFLRELEANME------NVAHAFLKMAPFF-KLYSVYAFDYRGALFIIQDL 170
            .||.::..:.:.:..||.:||..:|      :|....|...|.| ::|..|..:........|.|
  Rat   505 WLFSKLPEVKSTSERFLHDLEQRLEADVLRFSVCDVVLHHCPAFRRVYLPYVTNQAYQERTYQRL 569

  Fly   171 ISKNPVFRKFLEQTESRPEVQR-KLNSLMIVPIQRVPRYKLLLEQVLLYTSPADADYKLLKESVK 234
            :.:||.|...|.:.|..|..|| .|.|.:|:|.|||.|.|:|:|.:|..|:|...|..:..::..
  Rat   570 LLENPKFPGILARLEESPVCQRLPLTSFLILPFQRVTRLKMLVENILKRTTPGSQDEDMATKAFS 634

  Fly   235 EIEATASHINTCVEEQEITQYLIHLQNSL--VNRTPNIVKPSRRVIKEGVL-------------Q 284
            .::......|..|:..:.|:.||||...:  ..:...::..:|.:::.|.|             .
  Rat   635 ALKELVQECNASVQSMKRTEELIHLSKKIHFEGKIFPLISQARWLVRHGELVELAPLPAAPPAKL 699

  Fly   285 KITHKGTEIKRY--CVLMS--------DIFMYCKMIKERAPNTVVENSLECCCIFPLKKCKVYEM 339
            |::.|...:..:  |:|:|        .:|::..|    |...|.:.||:            .:.
  Rat   700 KLSSKAVYLHLFNDCLLLSRRKELGKFAVFVHANM----AELQVRDLSLK------------LQG 748

  Fly   340 LPGNFKLTCQSDG------IIFGSGDVQLSRTWVGFIRDAI---DLHVQCRKTLRKDSSKRTPIR 395
            :||:..|.....|      ::..:......:.|:..:|.:.   |..:.|      |...|..::
  Rat   749 IPGHVFLLRLLHGQRARHQLLLRARTESEKQRWISALRPSSPQEDKEITC------DGEDRPQVQ 807

  Fly   396 KKDMKKFGADYVLSPNKRKCEYDTVFRNKNRSTDSEEETEDSACFSRKRKVASGILRAPNGN--- 457
            .....|     .|.|::...|...:...|.|::|...|               |: |..:|.
  Rat   808 CVRTYK-----ALQPDELTLEKTDILAVKTRTSDGWLE---------------GV-RLADGEKGW 851

  Fly   458 -PMGKASSSSSSSVSM------KRVAPPPPAPPPPPA 487
             |.......||.|..:      |||:........|||
  Rat   852 VPQAHVEEISSLSARLRNLREYKRVSNVSSKLGDPPA 888

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	47/177 (27%)
		PH-like	269..>311	CDD:302622	10/64 (16%)
		PH	277..374	CDD:278594	19/125 (15%)
Arhgef19	NP_001102162.1	RhoGEF	468..645	CDD:279015	46/176 (26%)
		PH_ephexin	666..792	CDD:269929	20/141 (14%)
		PH	679..786	CDD:278594	18/122 (15%)
		SH3	805..859	CDD:302595	12/74 (16%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810