DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF4 and Fgd5

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	NP_001102107.2	Gene:	Fgd5 / 362402	RGDID:	1305589	Length:	1520	Species:	Rattus norvegicus

Alignment Length:	533	Identity:	103/533 - (19%)
Similarity:	193/533 - (36%)	Gaps:	132/533 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly    76 QEIISSEKSYLEQLE-LLMNFFVRPLKEQAIIDCSNHTLL--------FGQIEMIHNLNGEFLRE 131
            ||::||||:|::.|: |.::|....|:....::......|        ..::..|.:|:...|..
  Rat   953 QELLSSEKAYVQMLQHLSLDFHGAVLRALENVEQEGREPLAQEELRQGLRELPAICDLHQGILES 1017

  Fly   132 LEANM-------ENVAHAFLKMAPFFKLYSVYAFDYRGALFIIQDLISKNP----VFRKFLEQTE 185
            ||..:       :.||..||.....|:.::.:...:...|.::.:....:|    ..|:|.:|:.
  Rat  1018 LEQRLGDCGEGQQQVADIFLAREQEFEHHAAHILQFDRYLGLLAESCLLSPRLATTVREFEQQSS 1082

  Fly   186 SRPEVQRKLNSLMIVPIQRVPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATASHINTCVEEQ 250
            .......|...|.:|  ||:.:|::||...|....|..|:|...:.::..|.......|..:|:.
  Rat  1083 QGGGQSMKHRMLRVV--QRLFQYQVLLTDYLNNLCPDSAEYDNTQSALNLISKVTDRANESMEQG 1145

  Fly   251 EITQYLIHLQNSLVNRTPNIVKPSRRVIKEGVLQKITHKGTEIKRYCVLMSDIFMY--------- 306
            |..|.|:|::.| |....::::|.|..:|||.|.::..|... .|:..||:|..:|
  Rat  1146 ENLQKLVHIEYS-VRGQGDLLQPGREFLKEGTLMRVRGKSRH-PRHLFLMNDTLLYTHPQKDGKY 1208

  Fly   307 -------------CKMIKERAPNTVVENSLECCCIFPLKKCK--------VYEMLPGNFKLTCQS 350
                         .:.:.::.|..:...:.|.|.......|.        :...||.::|....:
  Rat  1209 RLKSSLPVANMKVSRPVMDKVPYALKIETPESCLTLSASSCAERDEWHYCLSRALPEDYKTQALA 1273

  Fly   351 DGIIFGSGDVQLSRTWVGFIRDAIDLHVQCRKTLRKDSSKR----TPIRKKDM-KKFGADYVLSP 410
                                  |....|:.|:.|.....:|    .|:....| ...|.|:.|:.
  Rat  1274 ----------------------AFHHSVEIRERLGISLGERLPTLVPVTHAMMCMNCGCDFSLTV 1316

  Fly   411 NKRKCEY--DTVFRN-----------KNRSTDSEEETEDSACFSRKRKVASGILRAP-------N 455
            .:..|..  ..|.||           |||.....:     .|| |:.|:.:|.:..|       .
  Rat  1317 RRHHCHACGKIVCRNCSRNKYPLKCLKNRMAKVCD-----GCF-RELKLRNGPVPGPIRERPVSM 1375

  Fly   456 GNPMGKASSSSSSSVS--MKRVAPPPPAPPPPPAVQMRHQPGRPGELATASKENRISKLYKKIAA 518
            ..|:..:..||.|:.|  .:.::|                       :|..|:.::.....::||
  Rat  1376 SFPLSSSRFSSGSTFSSVFQSISP-----------------------STFKKQKKVPSALAEVAA 1417

  Fly   519 GDKVANVRGILKR 531
            ..:.:.:.|.|.|
  Rat  1418 SGEGSAISGYLSR 1430

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	40/187 (21%)
		PH-like	269..>311	CDD:302622	12/63 (19%)
		PH	277..374	CDD:278594	17/126 (13%)
Fgd5	NP_001102107.2	PHA03169	113..>324	CDD:223003
		RhoGEF	951..1139	CDD:366202	40/187 (21%)
		PH-like	1158..1280	CDD:388408	21/144 (15%)
		FYVE_like_SF	1294..1360	CDD:333710	16/71 (23%)
		PH2_FGD5_FGD6	1424..1514	CDD:270057	3/7 (43%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X316
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.910