Protein Alignment RhoGEF4 and Fgd3

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: XP_008769765.1
• Gene: Fgd3 / 361223
• RGDID: 1311725
• Length: 733
• Species: Rattus norvegicus
• Alignment Length: 581
• Identity: 133/581 - (22%)
• Similarity: 225/581 - (38%)
• Gaps: 130/581 - (22%)

  Fly    60 PNPDAGKRLGFRRQAI----QEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHTLLFGQIEM 120
            |..|.|.......|.:    ||::.:|::|:::|.||...|...|.| |.|.....|.:|..|..
  Rat   139 PEEDVGSTQCSDPQKLLHIAQELLHTEEAYVKRLHLLDQVFCAKLTE-AGIPPEVTTGIFSNISS 202

  Fly   121 IHNLNGEFL--------RELEANMENVAHAFLKMAPFFKLYSVYAFDYRGALFIIQDLISKNPVF 177
            |:..:|:||        .|.......:.....|:|||.|:|..|..::..|:.::.....::|.|
  Rat   203 IYRFHGQFLLPGLKKRITEEWDTKPRLGDILQKLAPFLKMYGEYVKNFDRAMGLVSTWTQRSPQF 267

  Fly   178 RKFLEQTESRPEV--QRKLNSLMIVPIQRVPRYKLLLEQVLLYTSPADA-DYKLLKESVKEIEAT 239
            :..: .|..:.||  ...|...|:.|:||||||:|||:. .|...|.|| |.|..:.|::.|...
  Rat   268 KDVI-HTIQKQEVCGNLTLQHHMLEPVQRVPRYELLLKD-YLKRLPRDAPDRKDAERSLELISIA 330

  Fly   240 ASHINTCVEEQEITQYLIHLQNSLVNRTPNIVKPSRRVIKEGVLQKITHK-GTEIKRYCVLMSDI 303
            |.|.|..:.:.|....|:.:...| ....:||.|:..:||||.:||::.| ||...|:..|.:::
  Rat   331 ADHSNAAIRKMEKMHKLLEVYEQL-GGEEDIVNPANELIKEGNIQKLSAKNGTTQDRHLFLFNNV 394

  Fly   304 FMYC-KMIKERAPNTVVENSLECCCIFPLKKCKVYEMLPGN----FKLTCQSDGIIFGSGDVQLS 363
            .:|| ..::.......|...::      :...:|.:::..|    |.:|.:...:...:...:..
  Rat   395 MLYCVPKLRLMGQKFSVREKMD------ISDLQVQDVVKPNAARTFIITGRKRSLELQTRTEEEK 453

  Fly   364 RTWVGFIRDAIDLHVQCRKTLR-------------------------------KDSSKRTP---I 394
            :.|:..|:..::.|.|..:|.|                               .|||..||   .
  Rat   454 KEWIQVIQATVEKHKQKSETFRAFSGACTQEEEPSLSPDQPVLSAGPVEPAGVADSSGGTPGIES 518

  Fly   395 RK------KDMKKFG----ADYVLSPNKRK----------CEYDTVFRNKNRSTDSEEETEDSAC 439
            ||      :|.:|.|    .:...|..||:          |...:.|:.:|    |::......|
  Rat   519 RKLSSKTRRDKEKPGCKSCGETFNSITKRRYRCKLCGEVICRKCSEFKAEN----SKQSRVCREC 579

  Fly   440 FSRKRKVASGILRAPNGNPMGKASSSSSSSVSMKRVAPPPPAPPPPPAVQMRHQPGRPGELATAS 504
            |..:              |:...|.||.:...:|:.|..||:..|.|::..       |.|..:.
  Rat   580 FLEE--------------PLLPTSPSSETPTELKQNAEKPPSVDPRPSLLC-------GTLNLSD 623

  Fly   505 KENRISKLYKKIAAGDKVANVRGILKRS--------------NVPAPNNDHDPSYGFASRY 551
            .....|:::..|...|  ..|..:|..|              |:..|    ||..|..:.|
  Rat   624 NGTAWSEVWAAIPESD--PQVLDLLAGSQAGKLLYSIPLSGCNITVP----DPEEGLEAGY 678

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	55/184 (30%)
		PH-like	269..>311	CDD:302622	16/43 (37%)
		PH	277..374	CDD:278594	20/102 (20%)
Fgd3	XP_008769765.1	RhoGEF	157..336	CDD:279015	55/181 (30%)
		PH-like	367..474	CDD:302622	22/112 (20%)
		PH	367..465	CDD:278594	20/103 (19%)
		FYVE_FGD3	527..580	CDD:277279	10/56 (18%)
		PH2_FGD1-4	606..710	CDD:270056	17/86 (20%)
		PH	622..711	CDD:278594	12/63 (19%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X316
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.810