Protein Alignment RhoGEF4 and cyst

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: NP_001260588.1
• Gene: cyst / 35237
• FlyBaseID: FBgn0032796
• Length: 1319
• Species: Drosophila melanogaster
• Alignment Length: 885
• Identity: 165/885 - (18%)
• Similarity: 290/885 - (32%)
• Gaps: 328/885 - (37%)

  Fly     5 RQKGYGNGFIHSPQTPLSFSRELRQVLQERNLLTAKSRRKVCSMLDSNNSSPIGSPNPDAGKRLG 69
            ::.|.|..:...|... :||....|.|             :.|.|...|.:||.:.:..|...||
  Fly   365 KKMGGGQDYYDGPDAS-TFSNPEDQPL-------------IRSDLQFLNEAPIEAQDLGADPVLG 415

  Fly    70 F--------------------------RRQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDC 108
            .                          |::.|.|.|.:||.:.:.|.::...||..|:..  ...
  Fly   416 IVLKEHDSWTPNVPRELLKTLKDLQIKRQEHIYEFIMTEKHHCQTLIVMQKVFVESLERH--FPS 478

  Fly   109 SNHTLLFGQIEMIHNLNGEFLREL-----EANM-ENVAHAFLKMAPFFKL---------YSVYAF 158
            .|...:|.:::.:.:|:..|||:|     |.:| :::|...|   .||.|         |..:..
  Fly   479 LNLNSMFPRLQQLTDLHTCFLRQLRLKQREQHMVDSIADILL---DFFSLEQAQCLRLAYGEFCA 540

  Fly   159 DYRGALFIIQDLISKNPVFRKFLEQTESRPEVQRK-LNSLMIVPIQRVPRYKLLLEQVLLYTSPA 222
            ::|.||...: |....|.|.::.:.....|.:::| :...::...||:.:|.||:|.:|......
  Fly   541 NHRSALDQFK-LCLTEPSFAEWYKHCLQNPLLKKKGIPECILFVTQRLTKYPLLIEPLLKSARDN 604

  Fly   223 DADYKLLKESVKEIEATASHINTCVEEQEITQYLIHLQNSLVNRTPNIV--KPSRRVIKEGVLQK 285
            ..:...|:.::..:::....::.||.|:|:.:..:.:...:..::..|.  ||.|:
  Fly   605 KLETDKLQHALNLVKSLLVDVDACVAEKELRERQLEIYGRVDAKSFAIYKNKPFRK--------- 660

  Fly   286 ITHKGTEIKRYCVLMSDIFMYCKMIKERAPNTVVENSLECCC---------------------IF 329
             |..|.|.:|.  |..|........:.:....:|....:|.|                     :.
  Fly   661 -TELGVESRRR--LKFDGLATLMQGRAKTQLVLVVVLTDCLCFLSENSGHNKYSFFTPEHKAGVV 722

  Fly   330 PLKKCKVYEMLPGNFKLTCQSDGIIFGSGD----------VQLSR---TWVGFIRDAIDLHVQCR 381
            ||:|..:.|      |...:|.||...|.:          ||..:   ||:..||.|:   :.|.
  Fly   723 PLQKLLIRE------KAGTESRGIYIISSNPDFPEMYELKVQTPKDKNTWIQTIRQAV---LDCP 778

  Fly   382 KT-------------LRKDSSKRTPIRK-----------------------KDMKKFG------- 403
            .|             ||...:||..|.|                       |:.:.||
  Fly   779 ATDIIEAEDLTAEEKLRIGVNKRETIEKMRQKDIEQALLLEEKLMLQFNLLKEQQPFGDVAGTNS 843

  Fly   404 ----ADYVLSPNKRK------CEYDTVFRN-------------------------KNRSTDSEEE 433
                |:::.:....|      |:...::|.                         :..|:..|::
  Fly   844 SCSAANFLAAFGSYKDLVSADCDTAELWRRVLNTVQDISQLASTIYSAATGQPVPRTLSSVGEKQ 908

  Fly   434 TEDSAC------------FSRKR-KVASGILRAPNGNPM--GKASSSSSSSVS------------ 471
            :|..|.            |..|| |:||.::..|....:  .:...|.|||||
  Fly   909 SEQYASPTLPKRAETFAGFDEKRGKLASKLVLTPRLQELEEKREPKSGSSSVSEQPLENNYAVLQ 973

  Fly   472 ---------------------------MKRVAPPPPA---------------------------- 481
                                       :.|.:|...|
  Fly   974 VTHHLQTLLCVISQQMTTINNLQLQLALYRESPRSSAYTHKDQLEELRNLQDKQQEEKTAWQRQL 1038

  Fly   482 -PPPPPAVQMRHQPGRPGELATASKEN---RISKLYKKIAAGDKVANVRGILKRSNVPAP--NND 540
             .......:||.|..:..:...|.:|:   :..:||||:    ::.:.:|:|...:.|.|  :|:
  Fly  1039 KQQQEELAEMRAQQLQLQKQIKAEQEDVRQQREQLYKKM----ELLSSQGLLLSPSTPLPPVSNN 1099

  Fly   541 HDPSYGFASRYSDSKSYFRAHNVDNTIP-------------TFVKREDLFNGDNMFPVHLKSHEE 592
            |.|.   |:...||      |..||.:.             ||.:|::.:         |.....
  Fly  1100 HPPP---AALLDDS------HETDNGLSGSGTGSSTPSVGGTFDRRKEKW---------LSGSSS 1146

  Fly   593 ICTPPQKKRVKFDDTLDA--VSPVLVEK--PFAFSSESSS 628
            ..|||    |......:|  |:|.||::  |...||.|||
  Fly  1147 CKTPP----VNLISANNAPKVNPTLVKQKLPMKLSSLSSS 1182

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	40/185 (22%)
		PH-like	269..>311	CDD:302622	10/43 (23%)
		PH	277..374	CDD:278594	24/130 (18%)
cyst	NP_001260588.1	RhoGEF	447..627	CDD:279015	40/185 (22%)
		PH_ARHGEF2_18_like	671..774	CDD:275432	20/108 (19%)
		PH	681..774	CDD:278594	18/98 (18%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm46934
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	3	2.810