DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment RhoGEF4 and Prex1

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	NP_001129190.1	Gene:	Prex1 / 311647	RGDID:	1306534	Length:	1646	Species:	Rattus norvegicus

Alignment Length:	695	Identity:	142/695 - (20%)
Similarity:	261/695 - (37%)	Gaps:	153/695 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly    53 NSSPIGSPNPDAGKRLGFRRQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDC-------SN 110
            :|.|..:.. |:.::|..|...:.||:::|:.|:..|..|.:.|::.:: |.:.|.       .|
  Rat    28 SSGPCAAAR-DSERQLRLRLCVLNEILATERDYVGTLRFLQSAFLQRIR-QNVADSVDKGLTEEN 90

  Fly   111 HTLLFGQIEMIHNLNGEFLRELE-------ANMENVAHAFLKMAPFFKLYSVYAFDYRGALFIIQ 168
            ..:||..||.|..::.:||..||       .:...:.:.|||....|.:|..|..::..||.::.
  Rat    91 VKILFSNIEDILEVHKDFLAALEYCLHPEPQSQHELGNVFLKFKDKFCVYEEYCSNHEKALRLLV 155

  Fly   169 DLISKNPVFRKFL--------EQTESRPEVQRKLNSLMIVPIQRVPRYKLLLEQVLLYTSPADAD 225
            :| :|.|..|.||        .:|...|     |...::.||||:.:|.|||:::...|.....|
  Rat   156 EL-NKVPAARAFLLNCMLLGGRKTTDIP-----LEGYLLSPIQRICKYPLLLKELSKRTPGKHPD 214

  Fly   226 YKLLKESVKEIEATASHINTCVEEQEITQYLIHLQNSLVN-RTPNIVKPSRRVIKEGVLQKITHK 289
            :..::.:::.::...|:||....:.|..:.|..||:.:.. ...|:.....:::.:|.|.||: .
  Rat   215 HSAVQSALQAMKTVCSNINETKRQMEKLEALEQLQSHIEGWEGSNLTDICTQLLLQGTLLKIS-A 278

  Fly   290 GTEIKRYCVLMSDIFMYCKMIKERAPNTVVENSLECCCIFPLKKCKVYEMLPGNFKL-------- 346
            |...:|...|..::.:|||. |.|...:             .|..|..:.:.|:..:
  Rat   279 GNIQERAFFLFDNLLVYCKR-KSRVTGS-------------KKSTKRTKSINGSLYIFRGRINTE 329

  Fly   347 TCQSDGIIFGSGDVQLSRTWVGF-IRDAIDLH-------VQCRKTLRKDSSK--RTPIRKKDMK- 400
            ..:.:.:..|:.|...:    |: :.:...:|       ..|.....::..|  ...||:::.:
  Rat   330 VMEVENVEDGTADYHSN----GYTVTNGWKIHNTAKNKWFVCMAKTAEEKQKWLDALIREREQRE 390

  Fly   401 --KFGAD---YVLSPNKRKCEYDTVFRNK-NRSTDSEEETED-SACFSRKRKVA-----SGILRA 453
              |.|.:   ||:...|.:..|..:...| |...|...:... ..||.....||     ..|.:.
  Rat   391 SLKLGMERDAYVMIAEKGEKLYHMMMSKKVNLIKDRRRKLSTVPKCFLGNEFVAWLLEIGEISKT 455

  Fly   454 PNGNPMGKASSSSSSSVSMKRVAPPPPAPPPPPAVQMRHQPGRPGELATASKENRIS-------- 510
            ..|..:|:|...:.   .:..|:............:.|:..|.  ..|.:..|:.:|
  Rat   456 EEGVNLGQALLENG---IIHHVSDKHQFKNEQVMYRFRYDDGT--YKARSELEDIMSKGVRLYCR 515

  Fly   511 ----------------KLYKKIAAGDKVANVRGILKRSN-----------VPAPNNDHDPSYGF- 547
                            |.||.:..|.|:  |..:|.:.:           |...||      ||
  Rat   516 LHSLYAPVIKDRDYHLKTYKSVVPGSKL--VDWLLAQGDCQTREEAVALGVGLCNN------GFM 572

  Fly   548 -----ASRYSDSKSYFRAHNVDNTIPTFVKREDLFNG----DNMFPVHLKSHEEICTPPQKKRVK 603
                 .|.:.|...|||.|..:....|..|.:.|.|.    :|:..      :.:..|||:....
  Rat   573 HHVLEKSEFKDESQYFRFHADEEMEGTSSKNKQLRNDFKLVENILA------KRLLIPPQEDDYG 631

  Fly   604 FDDTLDAVSPVLVEKPFAFSSESSSALKPTSLR--ERIYDFFANL 646
            ||  |:..:..:|.|    |.:..|..:...|:  .:||....:|
  Rat   632 FD--LEEKNKAVVVK----SVQRGSLAEMAGLQAGRKIYSINEDL 670

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	47/191 (25%)
		PH-like	269..>311	CDD:302622	11/41 (27%)
		PH	277..374	CDD:278594	18/105 (17%)
Prex1	NP_001129190.1	RhoGEF	48..234	CDD:279015	48/192 (25%)
		PH_Collybistin_ASEF	239..391	CDD:269931	28/170 (16%)
		PH	278..384	CDD:278594	17/123 (14%)
		DEP	409..489	CDD:295306	13/82 (16%)
		DEP_2_P-Rex	501..593	CDD:239887	20/99 (20%)
		PDZ	630..697	CDD:214570	11/47 (23%)
		PDZ	714..768	CDD:238080
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.910