DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF4 and Plekhg5

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	XP_038965663.1	Gene:	Plekhg5 / 310999	RGDID:	1303132	Length:	1101	Species:	Rattus norvegicus

Alignment Length:	669	Identity:	142/669 - (21%)
Similarity:	249/669 - (37%)	Gaps:	170/669 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly    11 NGFIHSPQTPLSFSRELRQVLQERNLLTAKS-------RRKVCSMLDSNNSSPIGSPNPD-AGKR 67
            :||..|..:..:|.||:.::.|..:.|.|.|       .|::....||...........| :|.|
  Rat   349 SGFFSSSPSTGAFGREVDKMEQLESKLHAYSLFGLPRMPRRLRFDHDSWEEEEDDEEEEDNSGLR 413

  Fly    68 LG-------------FRRQ-----AIQEIISSEKSYLEQLELLMNFF---VRPLKEQAIIDCSNH 111
            |.             .|||     |:.|::.:|.||:.:|.::.|.|   :..|:|..::.....
  Rat   414 LEDSWRELIDGHEKLTRRQCHQQEAVWELLHTEVSYIRKLRVITNLFLCCLLNLQESGLLCEVEA 478

  Fly   112 TLLFGQIEMIHNLNG-----------EFLRELEANME--NVAHAFLKMAPFFKLYSVYAFDYRGA 163
            ..||..|..:..|:.           |..|...|.::  :....|......||.|..|..:..|.
  Rat   479 ERLFSNIPELARLHRGLWSSVMVPVLEKARRTRALLQPSDFLKGFKMFGSLFKPYIRYCMEEEGC 543

  Fly   164 LFIIQDLISKNPVFRKFLEQTESRPEVQR-KLNSLMIVPIQRVPRYKLLLEQVLLYTSPADADYK 227
            :..::.|:..|.:||.::...|...:.|| ||:.::..|.||:.:|.|||:.||..|....|...
  Rat   544 MEYMRSLLRDNDLFRAYVTWAEKHQQCQRLKLSDMLAKPHQRLTKYPLLLKSVLRKTDEPRAKEA 608

  Fly   228 LLKESVKEIEATASHINTCVEEQ------------------------EITQYL---IHLQNS--L 263
            :: ..:..:|....|:|||:.::                        |:.::|   :||..:  :
  Rat   609 II-TMISSVERFIHHVNTCMRQRQERQRLAGVVSRIDAYEVVEGSNDEVDKFLKEFLHLDLTAPM 672

  Fly   264 VNRTPNIVKPSRRVIKEGVLQKITHKGTEIKRYCVLMSDIFMYCKMIKERAPNTVVENSLECCCI 328
            ...:|   :.:|:::.||.|:....|.:::..||.|.:|:.:..|.:|:.....|:..
  Rat   673 PGTSP---EETRQLLLEGSLRMKEGKDSKMDVYCFLFTDLLLVTKAVKKAERTKVIRP------- 727

  Fly   329 FPLKKCKVY--EML-PGNFKLTCQSD------GIIFGSGDVQLSRTWVGFIRDAIDLHVQCR--- 381
             ||...|:.  |:. ||:|.|...::      ...|.:....|.|:||..:.:|.:...|.|
  Rat   728 -PLLVDKIVCRELRDPGSFLLIHLNEFHSAVGAYTFQASSQALCRSWVDTLYNAQNQLQQLRAQL 791

  Fly   382 ------------KTLRKDSSKRTPIRKKDMKKFGADYVLSPNKRKCEYDTVFRNKNRSTDSEE-- 432
                        ::|.::..:    ::::.::.|.....||        |:.|..:.|.|||.
  Rat   792 LCAQEHPGTQHLQSLEEEEDE----QEEEGEESGTSAASSP--------TILRKSSNSLDSEHCA 844

  Fly   433 ----------------ETEDSACFSR-----------KRKVASGILRA----PNGNPMGKASSSS 466
                            ||..|..|.|           .....|.|...    |.|...|::.|..
  Rat   845 SDGSTETLAMVVVEPGETLSSPEFDRGPFSSQSDEASLSNTTSSITPTSELLPLGPVDGRSCSMD 909

  Fly   467 S-----SSVSMKRVAPPPPAPPPPPAVQ-MRHQPG------RPGELATASKENRISKLYKKIAAG 519
            |     |..|::..|.|.|...|.|..| :..||.      .|.:|..     |:..|.|..:..
  Rat   910 SAYGTLSPTSLQDFAAPHPVVEPVPVPQTLSPQPSPRLRRRTPVQLLP-----RLPHLLKSKSEA 969

  Fly   520 DKVANVRGILKRSNVPAPN 538
            ..:..:.|.....:.|||:
  Rat   970 SLLQLLSGTTTSVSPPAPS 988

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	45/186 (24%)
		PH-like	269..>311	CDD:302622	10/41 (24%)
		PH	277..374	CDD:278594	24/105 (23%)
Plekhg5	XP_038965663.1	RBD_PLEKHG5	172..246	CDD:340588
		RhoGEF	438..624	CDD:214619	45/186 (24%)
		PH_PLEKHG5_G6	681..779	CDD:270064	25/105 (24%)
		Atrophin-1	913..>1077	CDD:397323	19/81 (23%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810