Protein Alignment RhoGEF4 and Net1

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: XP_006254268.1
• Gene: Net1 / 307098
• RGDID: 1305034
• Length: 595
• Species: Rattus norvegicus
• Alignment Length: 512
• Identity: 106/512 - (20%)
• Similarity: 195/512 - (38%)
• Gaps: 100/512 - (19%)

  Fly     2 FTPRQKGYGNGFIHSP-QTPLSFSRELRQVLQERNLLTAKSRRKVCSMLDSNNSSPIGSPNPDAG 65
            ||.|      |...|| .|..||||.......:|......|.....||.:|..:..|.
  Rat   123 FTLR------GDHRSPASTQKSFSRATVPTPTKRRSSALWSEMLDISMKESLTTREIK------- 174

  Fly    66 KRLGFRRQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHTLLFGQIEMIHNLNGEFLR 130
                 |::||.|:...|:..:|.|:|....:..|:.:.:|:.....|.:||.::....|:.:.|.
  Rat   175 -----RQEAIYELSRGEQDLIEDLKLARKAYHDPMLKLSIMSEEELTHIFGDLDAYIPLHEDLLA 234

  Fly   131 EL------EANMENVAHAFLKMAPFFKLYSVYAFDYRGALFIIQDLISKNPVFRKFLEQTESRPE 189
            .:      :..:|.:.|..:...|....|..|..:...|..:: |...::|..:.||::....|
  Rat   235 RIGEATKPDGTVEQIGHILVNWLPGLNAYRGYCSNQLAAKALL-DQKKQDPRVQDFLQRCLESP- 297

  Fly   190 VQRKLN--SLMIVPIQRVPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATASHINTCVEEQEI 252
            ..|||:  |.:.:|..|:.:|.|||:::|.:|.....|.:||::::..|:...|.||....|.|.
  Rat   298 FSRKLDLWSFLDIPRSRLVKYPLLLKEILRHTPKDHLDVQLLEKAILIIQEVLSDINLKKGESEC 362

  Fly   253 TQYLIHLQNSLVNRTPNIVKPSRRVIKEGVLQ-KITHKGTEIKRYCVLMSDIFMYCKMI--KERA 314
            ..|:..|:.....:....::.|:.::..|.|: |..|     |.|..|..||.:..:.:  .||.
  Rat   363 QYYIDKLEYLDEKQKDPRIEASKVLLCHGELKNKSGH-----KLYIFLFQDILVLTRPVTRNERH 422

  Fly   315 PNTVVENSLECCCIFPLKKCKVYEMLPGNFKLTCQSDGIIFG----------------------- 356
            ...|....:      |:::..:.::..|:.::.....| .||
  Rat   423 SYQVYRQPI------PVQELVLEDLQDGDVRMGGSFRG-AFGNSDKAKNIFRVRFQDPSPGQSHT 480

  Fly   357 --SGDVQLSRTWVGFIRDAI----------------DLHVQCRKTLRKDSSKRTPIRKKDMKKFG 403
              :.||...:.|...||.||                |||.:|.:......:.|...|.
  Rat   481 LQANDVFHKQQWFNCIRSAIAPFQRAASPLELQGLPDLHEECEENNPSAGNLRAQRRS------- 538

  Fly   404 ADYVLSPNKRKCEYDTVFRNKNRSTDSEEETEDSACFSRKRKVASGILRAPNGNPMG 460
               .:.|...:.::::|. ....|..:.|:|.:    ::..:...|:.||.:...:|
  Rat   539 ---CVLPGVMQVDHESVL-ECGSSVQTSEDTRN----AKAHRPQHGLRRARDKAQLG 587

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	42/177 (24%)
		PH-like	269..>311	CDD:302622	10/44 (23%)
		PH	277..374	CDD:278594	22/124 (18%)
Net1	XP_006254268.1	RhoGEF	178..354	CDD:214619	42/177 (24%)
		PH_Net1	368..502	CDD:270044	26/145 (18%)
		PH	387..501	CDD:278594	23/125 (18%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810