DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF4 and Arhgef18

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	XP_038945236.1	Gene:	Arhgef18 / 304193	RGDID:	1311448	Length:	1284	Species:	Rattus norvegicus

Alignment Length:	541	Identity:	86/541 - (15%)
Similarity:	191/541 - (35%)	Gaps:	123/541 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly    71 RRQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHTLLFGQIEMIHNLNGEFLRELEAN 135
            |:..:.|::.:|..::..|::::..:.|.|:|:........:.||...:.:.:::..||..|:..
  Rat   365 RQDVLYELMQTEAHHVRTLKIMLKVYSRALQEELQFSVQAVSRLFPCADDLLDMHSHFLARLKER 429

  Fly   136 MENVA-------HAFLKMAPFF-------------KLYSVYAFDYRGALFIIQDLISKNPVFRKF 180
            .:...       :...|:....             :.|.|:...::.|:...:.|:.::..|:..
  Rat   430 RQEFLEEDSDRNYVIQKIGDVLVQQFSGETGERMKEKYGVFCSGHKDAVGHYKLLLQQSKKFQNL 494

  Fly   181 LEQTESRPEVQR-KLNSLMIVPIQRVPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATASHIN 244
            :::..:...|:| .:...:::..||:.:|.:|:|:::..|.....|||.|.:::..|:...|.::
  Rat   495 IKKIGNFSIVRRLGVQECILLVTQRITKYPVLVERIIQNTEAGTEDYKDLSQALNLIKDIISQVD 559

  Fly   245 TCVEEQEITQYLIHLQNSLVNRTPNIVKPSRRVIKEGVLQKITH-------KGTEIKR---YCVL 299
            ..|.|.|..|.|..:...:.:::...:|..|...||.:||:..|       |.|..:.   ..||
  Rat   560 AKVSECEKDQRLKEIVAKMDSKSSGKLKNGRTFRKEDMLQRQLHLDSTLCWKSTSGRLKDVLAVL 624

  Fly   300 MSDIFMYCKMIKERAPNTVVENSLECCCIFPLKKCKVYEML---------------PGNFKL--- 346
            ::|:.:   :::|:....|..:......:..|:|..|.|:.               |..:::
  Rat   625 LTDVLL---LLQEKDQKYVFASVDSKPPVISLQKLIVREVANEEKAMFLISASMQGPEMYEIYTS 686

  Fly   347 -----------------TC--QSDGIIFGSGDVQLSRTWVGFIRD-------------------- 372
                             :|  :.:||...:.:.:::......:||
  Rat   687 SKEDRNIWMAHIRRAVESCPDEEEGIFLEAEERRMAEARTMKLRDFQERLSLKDQLIAQSLLEKQ 751

  Fly   373 ----------AIDLHVQCRKTLRKDSSKRTPIRKKDMKKFGADYVLSPNKRKCEYDTVFRNKNRS 427
                      .:|...|||...|........:|.:.:.:.....:.......|      |:...:
  Rat   752 QIYLEMAQLSGLDESAQCRGLFRGGGDPSETLRGEQILRSAMSEIEGIQSLIC------RHVGST 810

  Fly   428 TDSEEETEDSACFSRKRKVASGILRAPNGNPMG----------------KASSSSSSSVSMKRVA 476
            :...||...||...|:.:...|.....:.|..|                :..:||..|:......
  Rat   811 SSQVEEGGVSAGLPRRAETFGGYDSVGSPNKGGSFKRKVYNSDPRPQDWQGPASSPDSMPCDTTT 875

  Fly   477 PPPPAPPPPPAVQMRHQPGRP 497
            |.......|.||:|......|
  Rat   876 PSGCCEESPQAVEMPSAESSP 896

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	31/190 (16%)
		PH-like	269..>311	CDD:302622	12/51 (24%)
		PH	277..374	CDD:278594	22/173 (13%)
Arhgef18	XP_038945236.1	C1	175..>200	CDD:412127
		RhoGEF	365..559	CDD:238091	32/193 (17%)
		PH_ARHGEF18	599..717	CDD:275437	17/120 (14%)
		DUF5401	<924..>1047	CDD:375164
		VirB10_like	1004..>1219	CDD:421703
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810