Protein Alignment RhoGEF4 and Pura

DIOPT Version :10

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: NP_996016.2
• Gene: Pura / 2768948
• FlyBaseID: FBgn0035802
• Length: 1904
• Species: Drosophila melanogaster
• Alignment Length: 650
• Identity: 130/650 - (20%)
• Similarity: 223/650 - (34%)
• Gaps: 200/650 - (30%)

  Fly    75 IQEIISSEKSYLEQL---------ELLMNFFVRPLKEQAIIDCSNHTLLFGQIEMIHNL-NGEFL 129
            ::|:|.:|:.|:..|         |||.....:||:.|       ..::||.||.|... |..||
  Fly  1354 MREMIGTERDYVRSLYYVIENYIDELLREDIPQPLRGQ-------RNVIFGNIEKIFEFHNSHFL 1411

  Fly   130 RELEANMEN---VAHAFLKMAPFFKLYSVYAFDYRGALFIIQDLISKN-PVFRKFLEQTES---R 187
            .|||....|   |..|||:|...|.||::|               :|| |.....|.:..|   :
  Fly  1412 GELERYERNPLKVGAAFLEMESKFYLYALY---------------NKNKPKSDTLLSEYGSSFFK 1461

  Fly   188 P---EVQRKLN--SLMIVPIQRVPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATA-SHINTC 246
            |   ::|.||:  |.::.|:||:.:|.|||:|              |.::.|.:|..| ..|...
  Fly  1462 PKQMQLQDKLDLASYLLKPVQRMGKYALLLQQ--------------LVKACKGVEGAALQEIAAD 1512

  Fly   247 VEEQEITQYLIHLQ----------NSLVNRTPNIVKPSRRVIKEGVLQKITHKGTEIKRYCVLMS 301
            |||.:..:.::..|          :||.:...|:.:..|.:.:...|......|.:..|...|..
  Fly  1513 VEELQRAEEMVKFQLRHGNDLLAMDSLRDCDVNVKEQGRLLRQNEFLVWQGRGGKKTLRQVFLFE 1577

  Fly   302 DIFMYCKMIKERAPN------TVVENSLECCCI------------FPL--KKCKVYEMLPGNFKL 346
            ::.::.|  ..|.|:      .:.:||::...|            |.:  :|.|..:    .:.|
  Fly  1578 ELVLFSK--ARRFPDHKNLDIYIYKNSIKTSDIGLTAHTGDSATKFEIWFRKRKPDD----TWML 1636

  Fly   347 TCQSDGIIFGSGDVQLSRTWVGFIRDAIDLHVQCRKTLRKDSSKRTPIRKKDMKKFGADYVLSPN 411
            .|.|:.|         ...|.          .:..|.|.|.:.:...:|..:|...|.      .
  Fly  1637 QCMSEDI---------KNAWT----------EEISKLLWKQAKRNREVRLAEMSSMGI------G 1676

  Fly   412 KRKCEYDTVFRNKNRSTDSE------EETEDSACFSRKRKVASGILRAPNGNPMGKASSSSSSSV 470
            .:.|.  .:..:.|:.:|..      .:|..    .|..:...|.:|.||......:.||.:|:.
  Fly  1677 SKPCL--DIRPSNNQISDRSIPLAQLNKTPK----LRHSEPGKGSMRRPNSLISESSLSSGTSTT 1735

  Fly   471 SMKRVAPPPPAPPPPPAVQMRHQPG----------RPGELATAS----------KENRISKLYKK 515
            |...::         ......|..|          .||..:.:|          .|.:..||.|.
  Fly  1736 SGSSIS---------GGSSSGHNDGGGRHSLHFGKLPGAHSASSTNCSATLELINETQALKLAKS 1791

  Fly   516 IAAGDKVANVRGILKRSNVPAPNNDHDPSYGFASRYSDSKSYFRAHNVDNTIPTFVKREDLFNGD 580
            .:.|       ||..........|......|..:.:..||   |.|....||.:.:..|.....|
  Fly  1792 PSGG-------GIKGADTAADGGNTSGNGNGNGNAFRSSK---RNHKRSTTIVSQLSMESGIVSD 1846

  Fly   581 NMFPVHLKSHEEICTPPQKKRVKFDD---------------TLDAVSPVLVE--KPFAFSSESSS 628
                        :|..|.:::...:.               :..:.|.|::.  :.:|.::||:|
  Fly  1847 ------------MCVTPDQEQSAAESSRSSWSTSTTGASGASTTSASTVILRRYRSYAHAAESAS 1899

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:459876	54/191 (28%)
		PH-like	269..>311	CDD:473070	7/41 (17%)
		PH	277..374	CDD:459697	18/116 (16%)
Pura	NP_996016.2	COG4913	<1020..>1167	CDD:443941
		RhoGEF	1353..1524	CDD:459876	58/205 (28%)
		PH_puratrophin-1	1524..1663	CDD:270062	26/163 (16%)