DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment RhoGEF4 and Plekhg5

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	XP_006538986.1	Gene:	Plekhg5 / 269608	MGIID:	2652860	Length:	1103	Species:	Mus musculus

Alignment Length:	680	Identity:	146/680 - (21%)
Similarity:	248/680 - (36%)	Gaps:	191/680 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly    11 NGFIHSPQTPLSFSRELRQVLQERNLLTAKSRRKVCSML----------------DSNNSSPIGS 59
            :||..|..:..:||||:.::.|..:.|.|.|...:..|.                |..:....|.
Mouse   350 SGFFSSSPSTSAFSREVDKMEQLESKLHAYSLFGLPRMPRRLRFDHDSWEEEEEDDEEDEESSGL 414

  Fly    60 PNPDAGKRLG------FRRQ-----AIQEIISSEKSYLEQLELLMNFF---VRPLKEQAIIDCSN 110
            ...|:.:.|.      .|||     |:.|::.:|.||:.:|.::.|.|   :..|:|..::....
Mouse   415 RLEDSWRELTDGHEKLTRRQCHQQEAVWELLHTEVSYIRKLRVITNLFLCCLLNLQESGLLCEVE 479

  Fly   111 HTLLFGQIEMIHNLNG-----------EFLRELEANME--NVAHAFLKMAPFFKLYSVYAFDYRG 162
            ...||..|..|..|:.           |..|...|.::  :....|......||.|..|..:..|
Mouse   480 AERLFSNIPEIAKLHRGLWGSVMVPVLEKARRTRALLQPSDFLKGFKMFGSLFKPYIRYCMEEEG 544

  Fly   163 ALFIIQDLISKNPVFRKFLEQTESRPEVQR-KLNSLMIVPIQRVPRYKLLLEQVLLYTSPADADY 226
            .:..::.|:..|.:||.::...|...:.|| ||:.::..|.||:.:|.|||:.||..|     |.
Mouse   545 CMEYMRGLLRDNDLFRAYVTWAEKHQQCQRLKLSDMLAKPHQRLTKYPLLLKSVLRKT-----DD 604

  Fly   227 KLLKES----VKEIEATASHINTCVEEQEITQYL---------------------------IHLQ 260
            ...||:    :..:|....|:|||:.:::..|.|                           :||.
Mouse   605 PRTKEAIVTMISSVERFIHHVNTCMRQRQERQRLAGVVSRIDAYEVVEGSNDEVDKLLKEFLHLD 669

  Fly   261 NS--LVNRTPNIVKPSRRVIKEGVLQKITHKGTEIKRYCVLMSDIFMYCKMIKERAPNTVVENSL 323
            .:  :...:|   :.:|:::.||.|:....|.:::..||.|.:|:.:..|.:|:.....|:..
Mouse   670 LTAPMPGTSP---EETRQLLLEGSLRMKEGKDSKMDVYCFLFTDLLLVTKAVKKAERTKVIRP-- 729

  Fly   324 ECCCIFPLKKCKVY--EML-PGNFKLTCQSD------GIIFGSGDVQLSRTWVGFIRDAIDLHVQ 379
                  ||...|:.  |:. ||:|.|...::      ...|.:....|.|:||..|.:|.:...|
Mouse   730 ------PLLVDKIVCRELRDPGSFLLIYLNEFHSAVGAYTFQASSQALCRSWVDTIYNAQNQLQQ 788

  Fly   380 CRKTLRKDSSKRTPIRKKDMKKFGADYVLSPNKRKCEYD--------------TVFRNKNRSTDS 430
            .|..|   |::..|         |:.::.|..:.:.|.:              |:.|..:.|.||
Mouse   789 LRAQL---SAQEHP---------GSQHLQSLEEEEDEQEEEGEESGTSAASSPTILRKSSNSLDS 841

  Fly   431 EEETEDSACFSRKRKVA--SGILRAP--NGNPMGKASSSSSSSVSMKRV---------------- 475
            |..|.|.:..:....|.  ...|.:|  .|.|:...|..||.|.:...|
Mouse   842 EHCTSDGSTETLAMVVVEPGATLSSPEFEGGPVSSQSDESSLSNTASSVTPTSELLPLGPVDGRS 906

  Fly   476 ---------------------------APPPPAPPPPPAVQMRHQP-----GRPGELATASKENR 508
                                       ||.|..|.|.|:.::|.:.     .||..|..:..|..
Mouse   907 CSMDSAYGTLSPTSLQDFVAPHPVVEPAPVPQTPSPQPSPRLRRRTPVQLLPRPPRLLKSKSEAS 971

  Fly   509 ISKLYKKIAAGDKVANVRGILKRSNVPAPN 538
            :.:|.....|      .||:     .|||:
Mouse   972 LLQLLSGTPA------ARGV-----PPAPS 990

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	48/190 (25%)
		PH-like	269..>311	CDD:302622	10/41 (24%)
		PH	277..374	CDD:278594	25/105 (24%)
Plekhg5	XP_006538986.1	RBD_PLEKHG5	172..246	CDD:340588
		RhoGEF	440..626	CDD:214619	48/190 (25%)
		PH_PLEKHG5_G6	683..781	CDD:270064	26/105 (25%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	2	1.910