DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment RhoGEF4 and NGEF

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	NP_062824.2	Gene:	NGEF / 25791	HGNCID:	7807	Length:	710	Species:	Homo sapiens

Alignment Length:	290	Identity:	69/290 - (23%)
Similarity:	130/290 - (44%)	Gaps:	40/290 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly    72 RQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHTLLFGQIEMIHNLNGEFLRELEANM 136
            ::|:.|:::||.||.:.|.||::.|:...:.:.|:..|...:||..:..:..::..||.|||..|
Human   275 QEAMFELVTSEASYYKSLNLLVSHFMENERIRKILHPSEAHILFSNVLDVLAVSERFLLELEHRM 339

  Fly   137 -ENVAHA------FLKMAPFFKLYSVYAFDYRGALFIIQDLISKNPVFRKFLEQTESRPEVQ-RK 193
             ||:..:      :...|..|.:|..|..:........:.|:.:...||:.:.|.|..|:.: ..
Human   340 EENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCRGLP 404

  Fly   194 LNSLMIVPIQRVPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATASHINTCVEEQEITQYLIH 258
            .:|.:|:|.||:.|.|||::.:|..............::.||:|......|..|.:...|:.:|.
Human   405 FSSFLILPFQRITRLKLLVQNILKRVEERSERECTALDAHKELEMVVKACNEGVRKMSRTEQMIS 469

  Fly   259 LQNSL---VNRTPNIVKPSRRVIKEGVLQKI----THKGTEIKR-----YCVLMSDIFMYCKMIK 311
            :|..:   :...| |:..||.::|:|.||::    |.:....|:     |..|.:|:.:.|:.|
Human   470 IQKKMEFKIKSVP-IISHSRWLLKQGELQQMSGPKTSRTLRTKKLFHEIYLFLFNDLLVICRQI- 532

  Fly   312 ERAPNTVVENSLECCCIFPLKKCKVYEMLP 341
                              |..|.:|::..|
Human   533 ------------------PGDKYQVFDSAP 544

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	45/177 (25%)
		PH-like	269..>311	CDD:302622	13/50 (26%)
		PH	277..374	CDD:278594	15/74 (20%)
NGEF	NP_062824.2	Regulatory region, modulates activity toward RHOA, RAC1 and CDC42. /evidence=ECO:0000250	1..273
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..143
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	194..236
		RhoGEF	277..456	CDD:279015	45/178 (25%)
		PH_ephexin	477..603	CDD:269929	19/88 (22%)
		PH	490..596	CDD:278594	15/74 (20%)
		SH3_ephexin1	616..670	CDD:212872
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	687..710
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810