Protein Alignment RhoGEF4 and gef2

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: NP_592928.1
• Gene: gef2 / 2543168
• PomBaseID: SPAC31A2.16
• Length: 1101
• Species: Schizosaccharomyces pombe
• Alignment Length: 738
• Identity: 139/738 - (18%)
• Similarity: 265/738 - (35%)
• Gaps: 221/738 - (29%)

  Fly    41 SRRKVCSMLDSNNSSPIGSPNPDAG--KRLGFRRQAIQEIISSEKSYLEQLELLMNFFVRPLKEQ 103
            ||:|      :::.||..:.:.|||  ..:..::..:.|::.:|.:|:|:|..|:|.:..||::.
pombe   205 SRKK------TSSPSPSFASSKDAGTIPAIQKKKSLLIEMMETESTYVERLRHLVNDYALPLRDL 263

  Fly   104 AIIDCSNHTLLFGQIEM----------IHNLNGEFLRELEA-----NMENV-----AHAFLKMAP 148
            |    .:...|.|..|:          :..||..||.|.||     |.|::     ....|::|.
pombe   264 A----KSSKKLLGLYELNTLFPPCLNKLIQLNSAFLDEFEAIMSDLNFEDIDEKKFEEIDLRLAC 324

  Fly   149 FFKLYSVYAFDYRGALFIIQD--------LISKNPVFRKFLEQTESRPEVQRKLNSLMIVPIQRV 205
            .|:.: .:||......::.|.        :.||.|.|.:|.:|.:....:...|:.|::.|:||:
pombe   325 CFESH-FFAFSQHYPRYLEQSNDFGNVLKMASKIPKFVEFHDQVKLNANMNVGLSQLIMEPVQRI 388

  Fly   206 PRYKLLLEQVLLYTSPADADYKLLK--ESVKEIEATASHINTCVEEQEITQYLIHLQNSLVNRTP 268
            |||.|.|:|::|.|...:..:..::  |.:|.|....:     |:.:|.::....||:.:.:..|
pombe   389 PRYSLFLDQIILLTQEGECQHTYVRSVEIIKNIAEMPT-----VDAEERSRIFAGLQHIIPDLAP 448

  Fly   269 NIVKPSRRVI----------KEGVLQKITH----------------------------------- 288
            |::..||..|          |.|.|..|.:
pombe   449 NMISNSRNFIDCIDVTREFLKNGQLHLIPYTLILFNDRICLVQRRSKSSIASTILDLRKQNPRNS 513

  Fly   289 ---------------KGTEIKRYCVLMSDIFMYCK------------MIKERA------------ 314
                           :..|:.|..|..:.||:..|            ::|.|.
pombe   514 YSKEKRAQYIGSNMNEAVELTRSMVEENTIFLISKYASSPSFFNEYPILKFRCDFENVRTMDRFY 578

  Fly   315 ---PNTVVENSLECCCIFPLKKCKVYEMLPGNF------KLTCQSDGIIFGSGDVQLSR------ 364
               ...:..|..:..|: ...|...:.:...|:      |.:.:||.:...:.|..:.:
pombe   579 QSFQKALSMNKSQPSCL-SFSKLNDFVVFFNNYSRFEYEKESKRSDIVCICTNDANVDKHKFLQD 642

  Fly   365 --TWVGFIRDAIDLHVQCRKTLRKDSSKRTPIRKKDMKKFGADYVLSPNK-RKCEYDTVFRNKNR 426
              ..:.|.:...|.|:.....|.........|.|:|:::...:|:::..: ..|.    |.|:|.
pombe   643 GNIVITFFQQDEDFHLSFDSWLGVSLPTEAVIAKEDLREACLNYLINIKRLLLCP----FSNRNF 703

  Fly   427 S-----TDSEEETEDSACFSRKRKVASGILRAPNGNPMGKASSSSSSSVSMKRVAPPPPAPPPPP 486
            |     ::..:....:....||.:::.|          |:..|.|..|:|:.|
pombe   704 SSLDLYSNLIQHLLSANSSPRKSRLSFG----------GRPGSPSKISLSLNR------------ 746

  Fly   487 AVQMRHQPGRPGELATASKENRISKLYKKIAAGDKVANVRGILKRSNVPAPN---NDHDPSYGFA 548
               ..:|.|.....||..     |::|.              |..:|:...:   |.|:.|...|
pombe   747 ---FYNQGGLSKSCATLP-----SQMYN--------------LDHNNISQKSLKFNTHNTSKASA 789

  Fly   549 SRYSDS----KSYFRAH-NVDNTIPTFVKREDLFNGDNMFPVHLKS--HEEICT--PPQKKRVKF 604
            .:..:.    |..|:.| ::.|.:....::|.: .||.::...||.  :||:.:  ||......|
pombe   790 EKTVEHLEAFKGGFKYHTDLKNLLYPLSEKEKI-EGDELYDNILKETFNEELLSHYPPNIIYATF 853

  Fly   605 DDTLDAVSPVLVEKPFAFSSESS 627
            ...|.:    .:.:.|.....||
pombe   854 QKYLSS----FINRKFGVLLSSS 872

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	50/199 (25%)
		PH-like	269..>311	CDD:302622	14/113 (12%)
		PH	277..374	CDD:278594	22/197 (11%)
gef2	NP_592928.1	RhoGEF	237..423	CDD:279015	50/190 (26%)
		Nod1	824..967	CDD:293719	13/53 (25%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0008364
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	1.000