Protein Alignment RhoGEF4 and ARHGEF18

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: XP_005272521.1
• Gene: ARHGEF18 / 23370
• HGNCID: 17090
• Length: 1426
• Species: Homo sapiens
• Alignment Length: 563
• Identity: 103/563 - (18%)
• Similarity: 210/563 - (37%)
• Gaps: 122/563 - (21%)

  Fly    71 RRQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHTLLFGQIEMIHNLNGEFLREL-EA 134
            |:..:.|::.:|..::..|::::..:.|.|:|:..........||...:.:...:..||..| |.
Human   513 RQDVLYELMQTEVHHVRTLKIMLKVYSRALQEELQFSSKAIGRLFPCADDLLETHSHFLARLKER 577

  Fly   135 NMENVA------HAFLKMAPFF-------------KLYSVYAFDYRGALFIIQDLISKNPVFRKF 180
            ..|::.      :...|:....             :.|.|:...:..|:...:.|:.:|..|:..
Human   578 RQESLEEGSDRNYVIQKIGDLLVQQFSGENGERMKEKYGVFCSGHNEAVSHYKLLLQQNKKFQNL 642

  Fly   181 LEQTESRPEVQR-KLNSLMIVPIQRVPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATASHIN 244
            :::..:...|:| .:...:::..||:.:|.:|:|:::..|.....||:.|.:::..|:...|.::
Human   643 IKKIGNFSIVRRLGVQECILLVTQRITKYPVLVERIIQNTEAGTEDYEDLTQALNLIKDIISQVD 707

  Fly   245 TCVEEQEITQYL------------IHLQNSLVNRTPNIVKPSRRVIKEGVLQKITHKGTEIKRYC 297
            ..|.|.|..|.|            ..|:|.|..|..::::  |::..||:|...|..|.......
Human   708 AKVSECEKGQRLREIAGKMDLKSSSKLKNGLTFRKEDMLQ--RQLHLEGMLCWKTTSGRLKDILA 770

  Fly   298 VLMSDIFMYCKMIKERAPNTVVENSLECCCIFPLKKCKVYEML---PGNFKLTCQSDG----IIF 355
            :|::|:.:   :::|:....|..:......:..|:|..|.|:.   ...|.::....|    .|:
Human   771 ILLTDVLL---LLQEKDQKYVFASVDSKPPVISLQKLIVREVANEEKAMFLISASLQGPEMYEIY 832

  Fly   356 GSGDVQLSRTWVGFIRDAID-----------------LHVQCRKTLRKDSSKRTPIRKK------ 397
            .|.. :....|:..|:.|::                 ..|:.|.|..:|..:|..::.:
Human   833 TSSK-EDRNAWMAHIQRAVESCPDEEEGPFSLPEEERKVVEARATRLRDFQERLSMKDQLIAQSL 896

  Fly   398 --------DMKKFGADYVLSPNKRKCEYDTVFRNKNRSTDSEEETEDSACFSRKRKVASGILRAP 454
                    :|.:.|....| |..|     .:||..:.|...:.|....:..|....:.|.|.| .
Human   897 LEKQQIYLEMAEMGGLEDL-PQPR-----GLFRGGDPSETLQGELILKSAMSEIEGIQSLICR-Q 954

  Fly   455 NGNPMGKASSSSSSSVSMKRVAPPPPAPPPPPAVQMRHQPGRPGELATASKENRISKLYKKIAAG 519
            .|:..|:|....||:       .||............:.|.:.|...            ||:::.
Human   955 LGSANGQAEDGGSST-------GPPRRAETFAGYDCTNSPTKNGSFK------------KKVSST 1000

  Fly   520 D-KVANVRG-------ILKRSNVP-----------APNNDHDP 543
            | :..:.||       .|..|::|           ||..:.||
Human  1001 DPRPRDWRGPPNSPDLKLSDSDIPGSSEESPQVVEAPGTESDP 1043

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	33/190 (17%)
		PH-like	269..>311	CDD:302622	8/41 (20%)
		PH	277..374	CDD:278594	19/103 (18%)
ARHGEF18	XP_005272521.1	RhoGEF	513..707	CDD:238091	34/193 (18%)
		PH_ARHGEF18	747..865	CDD:275437	21/123 (17%)
		PH	<786..851	CDD:278594	12/65 (18%)
		DUF2570	1061..1156	CDD:305162
		PKK	1086..1193	CDD:289257
		DUF4200	1111..>1190	CDD:290574

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810