DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment RhoGEF4 and FGD1

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	NP_004454.2	Gene:	FGD1 / 2245	HGNCID:	3663	Length:	961	Species:	Homo sapiens

Alignment Length:	374	Identity:	97/374 - (25%)
Similarity:	164/374 - (43%)	Gaps:	71/374 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly    77 EIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHTL--------LFGQIEMIHNLNGEF-LREL 132
            |::.:||:|:.:|.||...|...|.|:|    .|.:.        :|..|..|:..:.:| |.||
Human   380 ELLQTEKAYVSRLHLLDQVFCARLLEEA----RNRSSFPADVVHGIFSNICSIYCFHQQFLLPEL 440

  Fly   133 EANME------NVAHAFLKMAPFFKLYSVYAFDYRGALFIIQDLISKNPVFRKFLEQTESRPEVQ 191
            |..||      .:.....|:|||.|:|..|..::..|:.::.....::..|:..:.      |||
Human   441 EKRMEEWDRYPRIGDILQKLAPFLKMYGEYVKNFDRAVELVNTWTERSTQFKVIIH------EVQ 499

  Fly   192 RK-------LNSLMIVPIQRVPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATASHINTCVEE 249
            ::       |...|:.|:||:|||:|||:..||.......|.|..::|::.|...|.|.|..:.:
Human   500 KEEACGNLTLQHHMLEPVQRIPRYELLLKDYLLKLPHGSPDSKDAQKSLELIATAAEHSNAAIRK 564

  Fly   250 QEITQYLIHLQNSLVNRTPNIVKPSRRVIKEGVLQKITHK-GTEIKRYCVLMSDIFMYC------ 307
            .|....|:.:. .|:....:||.|::.:||||.:.|::.| ||...||.:|.:|..:||
Human   565 MERMHKLLKVY-ELLGGEEDIVSPTKELIKEGHILKLSAKNGTTQDRYLILFNDRLLYCVPRLRL 628

  Fly   308 --KMIKERAPNTVVENSLECCCIFPLKKCKVYEMLPGNFKLTCQSDGIIFGSGDVQLSRTWVGFI 370
              :....||...|  :.:|      ||:..... ||..|.::.:...:...:...:..:.||..|
Human   629 LGQKFSVRARIDV--DGME------LKESSNLN-LPRTFLVSGKQRSLELQARTEEEKKDWVQAI 684

  Fly   371 RDAIDLHVQCRKTLR------------------KDSSKR--TPIRKKDM 399
            ...:..|.|..:|.:                  .|..||  ||||:|::
Human   685 NSTLLKHEQTLETFKLLNSTNREDEDTPPNSPNVDLGKRAPTPIREKEV 733

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	53/188 (28%)
		PH-like	269..>311	CDD:302622	17/50 (34%)
		PH	277..374	CDD:278594	26/105 (25%)
FGD1	NP_004454.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..353
		SH3-binding. /evidence=ECO:0000255	171..187
		RhoGEF	376..559	CDD:238091	53/188 (28%)
		PH1_FGD1	591..698	CDD:275392	28/115 (24%)
		PH	591..688	CDD:278594	26/105 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	702..726		3/23 (13%)
		FYVE_FGD1_2_4	725..789	CDD:277280	5/9 (56%)
		PH2_FGD1-4	815..920	CDD:270056
		PH	823..921	CDD:278594
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	925..961
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X316
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	4	3.770