Protein Alignment RhoGEF4 and Fgd4

DIOPT Version :10

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: NP_001288746.1
• Gene: Fgd4 / 224014
• MGIID: 2183747
• Length: 887
• Species: Mus musculus
• Alignment Length: 550
• Identity: 125/550 - (22%)
• Similarity: 210/550 - (38%)
• Gaps: 148/550 - (26%)

  Fly    33 ERNLLTAKSRRKVCSMLDSNNS--------------SPIGSPNPDAGKRLGFRRQAIQ------- 76
            |||.....|...:.:..|.|.|              |..|.|..||  .|..|...:.
Mouse   252 ERNETITDSASSIANSHDENASDSSCRTPGTDLGLPSKEGEPGMDA--ELQERENGVNTMGLDTL 314

  Fly    77 -------------------EIISSEKSYLEQLELLMNFFVRPLKEQA---IIDCSNHTLLFGQIE 119
                               |::.:|::|:.:|:||...|...|.|:|   .........:|..|.
Mouse   315 DQHHEVKETNEQKLHKIATELLLTERAYVSRLDLLDQVFYCKLLEEANRGSFPAEMVNKIFSNIS 379

  Fly   120 MIHNLNGEF-LRELEANME------NVAHAFLKMAPFFKLYSVYAFDYRGALFIIQDLISKNPVF 177
            .|:..:.:| |.|||..|:      .:.....|:|||.|:|..|...:..|:.:::.:..:.|.|
Mouse   380 SINAFHSKFLLPELEKRMQEWETTPRIGDILQKLAPFLKMYGEYVKGFDNAVELVKTMTERVPQF 444

  Fly   178 RKFLEQTESRPEVQRK-------LNSLMIVPIQRVPRYKLLLEQVLLYTSPADADYKLLKESVKE 235
            :...|      |:|::       |...|:.||||:|||::||:..|...||...|:...|:|::.
Mouse   445 KSVTE------EIQKQKICGSLTLQHHMLEPIQRIPRYEMLLKDYLKKLSPDSPDWNDAKKSLEI 503

  Fly   236 IEATASHINTCVEEQEITQYLIHLQNSLVNRTPNIVKPSRRVIKEGVLQKITHKGTEI-KRYCVL 299
            |...|||.|:.:.:.|..:.|:.:. .::....:||.||..:||||.:.|:..:.|.. :||..|
Mouse   504 ISTAASHSNSAIRKMENLKKLLEIY-EMLGEEEDIVNPSNELIKEGQILKLAARNTSAQERYLFL 567

  Fly   300 MSDIFMYCKMIKERAPN-TVVENSLECCCIFPLKKCKVYEM----LPGNFKLTCQSDGIIFGSGD 359
            .:::.:||      .|. ::|.:.........:...|:.|.    .|..|:::.:...:...:..
Mouse   568 FNNMLLYC------VPRFSLVGSKFTVRTRVGIDGMKIVETHNEEYPHTFQISGKERTLELQASS 626

  Fly   360 VQLSRTWVGFIRDAIDLHVQCRKTLRK---------------DSSKRTP--IRKKDM-------K 400
            .|....|:..::::||...|..:|.|.               :..||.|  ||..::       :
Mouse   627 EQDKEEWIKALQESIDAFHQRHETFRNAIAKENDIPLEVSTAELGKRAPRWIRDNEVTMCMKCKE 691

  Fly   401 KFGA--------------------DYVLSPNKRKCEYDTVFRNK---------NRSTDSEEETED 436
            .|.|                    ||     |.:.|||....||         :..|||||
Mouse   692 SFNALTRRRHHCRACGHVVCWKCSDY-----KAQLEYDGGRLNKVCKDCYQIISGFTDSEE---- 747

  Fly   437 SACFSRKRKVASGILRAPNGNPMGKASSSS 466
                 :||:   |||...:....|.:...|
Mouse   748 -----KKRR---GILEIESAEVSGNSEVCS 769

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:459876	53/212 (25%)
		PH-like	269..>311	CDD:473070	15/42 (36%)
		PH	277..374	CDD:459697	19/102 (19%)
Fgd4	NP_001288746.1	RhoGEF	328..512	CDD:238091	53/189 (28%)
		PH1_FDG4	544..637	CDD:275434	19/98 (19%)
		FYVE_FGD1_2_4	675..739	CDD:277280	13/68 (19%)
		PH2_FGD1-4	758..860	CDD:270056	1/11 (9%)