Protein Alignment RhoGEF4 and tag-52

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: NP_508633.1
• Gene: tag-52 / 180657
• WormBaseID: WBGene00006431
• Length: 665
• Species: Caenorhabditis elegans
• Alignment Length: 572
• Identity: 139/572 - (24%)
• Similarity: 234/572 - (40%)
• Gaps: 121/572 - (21%)

  Fly    73 QAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHTLLFGQIEMIHNLNGEFLRELEANME 137
            |.:|||..:|::|::.|.::...|::.|.............:||:|..|...:...|..|:.  :
 Worm    57 QILQEIYDTERTYVKALTIVDEIFIQQLNLWCPKSKKCFRSMFGEISAICKTHQILLESLQC--K 119

  Fly   138 NVAHAFLKMAPFFKLYSVYAFDYRGALFIIQDLISKNPVFRKFLEQTESRPEVQ-RKLNSLMIVP 201
            .:|..|.|..||.|||:.||..|..:|.|...|:|:|. |||.|.:.|..|.|. :||.:.:|:|
 Worm   120 PIAEVFTKFVPFLKLYTSYASHYPNSLKIFAKLMSRND-FRKALTKIEEDPRVDGKKLQTYLIMP 183

  Fly   202 IQRVPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATASHINTCVEEQEITQYLIHLQNSLVNR 266
            |||:|||.:|::.::.|::..| |...|.:::..::..:..|.:|:...|..:.|:.:|.:. ..
 Worm   184 IQRIPRYIMLIQNLMNYSTEKD-DMTNLYKALSGMQGLSDQIQSCMTAYENGERLLQIQLNF-GL 246

  Fly   267 TPNIVKPSRRVIKEGVLQKITHKGTEIKRYCV--LMSDIFMY----------CKMIKERAPNTVV 319
            ..:|::|.|.::|||:|.|....|:...:..|  |.:||.:|          ||
 Worm   247 DGHILEPGRVLLKEGMLYKRELNGSANYQETVVFLFNDILLYGIKRISVMPLCK----------- 300

  Fly   320 ENSLECCCIFPLKKCKV-YEMLPGNFKLTCQSDGIIFGSGDVQLSRTWVGFIRDAIDLHVQCRKT 383
               .|...|..|:.|.| .:.:.|:..:.|...|:.|.:..:.....|...|.||||...:.|.|
 Worm   301 ---YEPSAILSLRHCIVQLDDIGGSIFIRCGDVGVDFTAYSLGGVTEWYNEICDAIDNAKKLRDT 362

  Fly   384 LRKDSSKRTPIRKKDMKKFGADYVLSPNKRKCEYDTVFRNKNRSTDSEEETED-----SACFSRK 443
            |||:|.|                     ::...:|..|..:||.......|.|     |...:||
 Worm   363 LRKESFK---------------------QKSLIFDKGFWQRNRDKLRNMITADMTGNISRVLTRK 406

  Fly   444 RKV----ASGILRAPNGNPMGKASSSSSSSVSMKRVAPPPPAPPPPPAVQMRHQPGRPGELATAS 504
            :..    .|..||       |:.::|:.:|...||                          ..|:
 Worm   407 KPAPKLEESDFLR-------GQWTTSTIASNQRKR--------------------------KVAT 438

  Fly   505 KENRISKLYKKIAAGDKVANVRGILKRSNVPAPNNDHDPSYGF-ASRYSDSKSYFRAHNVDNTIP 568
            .|:....::..:::.:.....|....:.|.|:       ..|: ||..|.|....||..:.|
 Worm   439 LEDEEEDVFNMLSSPNLSQRKRKATLQENTPS-------GVGWTASPRSSSFRKRRASTLRN--- 493

  Fly   569 TFVKREDLFNGDNMFPVHLKSHEEICTPP----------QKKRVKFDDTLDA 610
               |..|:.:|:| :..||..::...|.|          .|.|...||..|:
 Worm   494 ---KNADVTDGNN-YLSHLVLNDVTMTQPYTGLNAFVGTPKSRTPTDDDYDS 541

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	52/170 (31%)
		PH-like	269..>311	CDD:302622	16/53 (30%)
		PH	277..374	CDD:278594	25/109 (23%)
tag-52	NP_508633.1	RhoGEF	58..224	CDD:279015	52/169 (31%)
		PH-like	232..>318	CDD:302622	24/100 (24%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160158966
Domainoid	1	1.000	84	1.000	Domainoid score	I5293
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		oto17354
orthoMCL	1	0.900	-	-		OOG6_107378
Panther	1	1.100	-	-	LDO	PTHR12673
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R7163
SonicParanoid	1	1.000	-	-		X316
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	10	9.770