DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment RhoGEF4 and exc-5

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	NP_001294029.1	Gene:	exc-5 / 177692	WormBaseID:	WBGene00001366	Length:	861	Species:	Caenorhabditis elegans

Alignment Length:	471	Identity:	113/471 - (23%)
Similarity:	200/471 - (42%)	Gaps:	66/471 - (14%)

- Green bases have known domain annotations that are detailed below.

  Fly     7 KGYGNGFIHSPQTPLSFSRELRQVLQERNLLTAKSRRKVCSMLDSNNSSPIGSPNPDAGKRLGFR 71
            ||...||    |..||...|......|:........:...|.:||.:.....||..|..||....
Worm   273 KGPDGGF----QVKLSDGDEFELSEDEQEDDDTSLSQSCYSEIDSESDITGPSPKTDLDKRYQKA 333

  Fly    72 RQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHTLLFGQIEMIHNL-NGEFLRELEAN 135
            ..|.:|::.||:.|:::|:||.:.|...|.::.:|.....|.|...:..::.. |..||.:|..:
Worm   334 WYAAKELVDSEQRYVDKLKLLGDTFRNRLIKEELITNDKITRLLANVSSLYQFHNTHFLPQLLES 398

  Fly   136 M------ENVAHAFLKMAPFFKLYSVYAFDYRGALFIIQDLISKNPVFRKFLEQTESRPEVQ-RK 193
            :      :.:|:...|.|||.|:||.|..:|..|..:.::| .|...|...:::.|.:.|.: ..
Worm   399 IRDWHTTKRIANVVRKQAPFLKMYSEYTNNYDRATKLFEEL-KKKKKFADVVKEIEKQAECEGLP 462

  Fly   194 LNSLMIVPIQRVPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATASHINTCVEEQEITQYLIH 258
            |...:|.|:|||.||:|||::...:..|:|.|:.....:::.:...|:|.|..:::.:....:|.
Worm   463 LGHHLICPVQRVMRYQLLLQEYKKHLQPSDVDFDDTTVALELVLQAAAHANEMMKKLDRFGKVIE 527

  Fly   259 LQNSLVNRTPNIVKPSRRVIKEGVLQKI--THKGTEIKRYCVLMSDIFMYCKMIKERAPNTVVEN 321
            :|..|.| :.::|.|.|.::|.|.:|||  |.:.|| :|:..|.:|:.:   :..||.
Worm   528 VQEQLGN-SISLVSPGRELLKSGSVQKISSTTEKTE-ERFIFLFNDLVI---LASERK------- 580

  Fly   322 SLECCCIFPLKKCKVYEMLPGNFKLTCQSDGI----------IFGSGD---VQL-------SRTW 366
                  :..:.|.|:..:...:....|:.|.:          ..|:|.   |:|       ...|
Worm   581 ------MIGVSKYKLRAVFSASHMQVCEGDNLEREHSFYLRGSDGTGPKRCVELFTPTQKEKNDW 639

  Fly   367 VGFIRDAID---LHVQCRKTLRKDSSKRTPIRKKDMKKFGADYVLSPNKRKCEYDTVFRNKNRST 428
            |..|...||   .|     :|...||.|:.|.:.:...     :.|.:...|:.:..|.::....
Worm   640 VDSIFSIIDEAKAH-----SLTFTSSSRSSISENNNNS-----IESKHCADCDIEFGFLSRGSKC 694

  Fly   429 DSEEETEDSACFSRKR 444
            ..........||.|:|
Worm   695 VKCSRRLCKKCFGRRR 710

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	49/177 (28%)
		PH-like	269..>311	CDD:302622	14/43 (33%)
		PH	277..374	CDD:278594	24/118 (20%)
exc-5	NP_001294029.1	RhoGEF	336..514	CDD:279015	49/178 (28%)
		PH	545..647	CDD:278594	24/118 (20%)
		PH1_FGD1-4_like	545..643	CDD:275423	23/114 (20%)
		FYVE	676..727	CDD:214499	6/35 (17%)
		PH-like	<773..845	CDD:302622
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X316
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.910