DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment RhoGEF4 and tag-77

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	NP_001294044.2	Gene:	tag-77 / 177656	WormBaseID:	WBGene00006450	Length:	1029	Species:	Caenorhabditis elegans

Alignment Length:	775	Identity:	145/775 - (18%)
Similarity:	277/775 - (35%)	Gaps:	211/775 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 QKGYGNGFIHSPQTPLS------FSRELRQVLQERNLLTAKSRRKVCSMLDS-NNSSPIGSPNPD 63
            :||..|....||.:.:|      |||.........::||:.|..:     || :.:||:  |:.:
Worm   306 RKGRHNSLFVSPTSGMSSSSTDDFSRITSMTSDRSSILTSHSGGE-----DSTDGASPV--PDYE 363

  Fly    64 AG--------KRLGFRRQAIQEIISSEKSYLEQLELL----MNFFVRPLKEQAIIDCSNHTLLFG 116
            .|        |:|.:......::.::...||:::.:|    |..|.:.:....:...:.|..:..
Worm   364 TGDEKDDQRLKKLHYAAVEFLKVQNNYVQYLKEMAVLYPEYMERFGKRVGRDLLASHNGHENVVL 428

  Fly   117 QIEM-------IHNLNGEFLREL-------EANMENVAHAFLKMAPFFKLYSVYAFDYRGALFII 167
            ||:.       ||.:   .|:|:       ::...|::......|.|.|....:..:....|..:
Worm   429 QIKKIMVQILPIHEM---LLKEIDKVCSNWDSRYPNMSKTIGTFADFLKCCQPFLDNKADFLNKL 490

  Fly   168 QDLISKNPVFRK--FLEQTE-----SRPEVQRKLNSLMIVPIQRVPRYKLLLEQVLLYTSPADAD 225
            ..|.:::..|.:  ::.:||     .:..|.::|:.:.    |...|||||:.:...|... |.|
Worm   491 LQLRNEDKEFDEATYMFETEVFKRGKKGAVIQQLDQVH----QNFMRYKLLMLRYSEYLID-DCD 550

  Fly   226 YK-LLKESVKEIEATASHINTCV---EEQEITQYLIHLQNSLVNRTPNIVKPSRRVIKEGVLQKI 286
            .| ..:|:::::|.....:|..:   ..:|:|:.....|...     |:::|.|.:|::..:.|.
Worm   551 EKEKAQEAIQKLENVTQAVNQKMGLPTTEELTKLYYRFQCQF-----NVLEPGRVLIRQSEVMKQ 610

  Fly   287 THKGTEIKRYCVLMSDIFMYCKMIKERAPNTVVENSLECCCIFPLKKCKVYEMLPGNFKLTC--- 348
            |.|..: .||.||.:|....|::  ..:....:..|..    .||:..| :|.:..:.::.|
Worm   611 TRKELQ-PRYLVLFTDYLWICRV--SSSGQFDINRSYR----IPLEYMK-FERMEEDERIRCLQI 667

  Fly   349 ----QSDGIIFGSGDVQLSRTWVGFIRDAIDLHVQCRKTLRKDSSKRTPIRKKDMKKFGADYVLS 409
                :|..|||.|...:  ..|.    |.:......||:.::..|.......::.||.....:|:
Worm   668 RSRVKSALIIFSSEKER--NQWT----DDLTKAQYDRKSYKRRQSTAVQRHDENKKKMNKLLMLT 726

  Fly   410 ---PNKRKCEYDTVFRNKNRSTDSEEETEDSACFSRKRKVASGILRAPNGN-----PMGKASSSS 466
               |:....|...:..:::||...|.:.|.|:.         .:....||.     ..|:.:.:.
Worm   727 PEVPDASDLERPQLIDSRSRSMSCESQDELSSV---------PVTPLDNGEIDETLGFGEVTRNG 782

  Fly   467 SSSVSMKRVAPPPPAPPPPPA---------------------VQMRHQPGRPGELATA------- 503
            |..        .||:....|.                     :..||.....|.|...
Worm   783 SGK--------KPPSEMIKPVWLPDNISNECLMEGCSTEFNIINRRHHCRDCGWLICKFCKGQAP 839

  Fly   504 ------SKEN-------RISKLYK---------KIAAGDKVANVRGILKRSN---------VPAP 537
                  :|:|       |..|.||         .|...|:...|: |.||::         ..||
Worm   840 LSKYDFTKQNVCSECFDRHYKAYKDGVLFPSKNMIVQSDETILVK-IGKRNDKEIVDPRKLFKAP 903

  Fly   538 NNDHDPSYGFASRYSDSKS-----------YFRAHNVDNTIPTFVKREDL--------FNGDNMF 583
            .|     |||..|..:.|.           .||:...:......::|:||        .:..::.
Worm   904 VN-----YGFRHRNVEEKRAQSIVFGRVYLSFRSRKTETVRHALLRRDDLKLVFYKAELDSKSVL 963

  Fly   584 PVHLKSHEEICTP---------PQKKRVKFDDTLDAVSPVLVEKPFAFSSESSSALKPTS 634
            .:.:..:....||         ..:.:::.|||.|.|        .:|..::|::.|..|
Worm   964 ELLIYGYFYRETPLDDGWLFELVHRNQIRTDDTKDDV--------ISFRVDNSASAKKWS 1015

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	35/195 (18%)
		PH-like	269..>311	CDD:302622	13/41 (32%)
		PH	277..374	CDD:278594	23/103 (22%)
tag-77	NP_001294044.2	RhoGEF	376..570	CDD:238091	36/201 (18%)
		PH1_FGD5_FGD6	588..712	CDD:275424	30/142 (21%)
		FYVE	793..860	CDD:214499	8/66 (12%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR12673
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X316
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	3.010