DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment RhoGEF4 and Itsn1

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	XP_006522994.1	Gene:	Itsn1 / 16443	MGIID:	1338069	Length:	1743	Species:	Mus musculus

Alignment Length:	430	Identity:	96/430 - (22%)
Similarity:	173/430 - (40%)	Gaps:	77/430 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly    62 PDAGKRLGFRRQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHTLLFGQIEMIHNLNG 126
            |...||.|:    |.|:|.:|::|:..|:|:...|.:||.|..::......::|...:.:...|.
Mouse  1255 PTERKRQGY----IHELIVTEENYVNDLQLVTEIFQKPLTESELLTEKEVAMIFVNWKELIMCNI 1315

  Fly   127 EFLRELEANME------------NVAHAFL-KMAPFFKLYSVYAFDYRGALFIIQDLISKNPVFR 178
            :.|:.|....:            ::..|.| .|.|:.:..|.   ...||. :||....:.|.|:
Mouse  1316 KLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSC---QLNGAA-LIQQKTDEAPDFK 1376

  Fly   179 KFLEQTESRPEVQ-RKLNSLMIVPIQRVPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATASH 242
            :|:::....|..: ..|:|.::.|:|||.||.|:::.:|..|.....|:..||.::::.|...|.
Mouse  1377 EFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQ 1441

  Fly   243 INTCVEEQEITQYL----IHLQ-----NSLV-NRTPNIVKPSRRVIKEGVLQKITHKGTEIKRYC 297
            :|..|.|:|.:..|    .|:|     ..|| |...|.:.| |:.:..|.|.| .....|:  |.
Mouse  1442 VNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGP-RKFLHSGKLYK-AKSNKEL--YG 1502

  Fly   298 VLMSDIFMYCKMIKERAPNTVVENSLECCCIFPLKKCKVYEMLPGNFKLTCQSDGIIF-----GS 357
            .|.:|..:..::.|....:...:       :|..|....|:|    :|.....:.::.     .|
Mouse  1503 FLFNDFLLLTQITKPLGSSGTDK-------VFSPKSNLQYKM----YKTPIFLNEVLVKLPTDPS 1556

  Fly   358 GDVQL------------------SRT-WVGFIRDAIDLHVQCRKTLRKDSSKRTPIRKKDMKKFG 403
            ||..:                  .|| ||..|:.|.:|:::..|..|:.:......|...:.:..
Mouse  1557 GDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLM 1621

  Fly   404 ADYVLSPNKRKCEYDTVFRNKNRSTDSEEETEDSACFSRK 443
            .:.|.....:.|      |:..:|....|.|..|.|...|
Mouse  1622 VNVVEGIELKPC------RSHGKSNPYCEVTMGSQCHITK 1655

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	44/183 (24%)
		PH-like	269..>311	CDD:302622	10/41 (24%)
		PH	277..374	CDD:278594	21/120 (18%)
Itsn1	XP_006522994.1	EH	39..132	CDD:197477
		PLN02983	<155..226	CDD:215533
		EH	238..333	CDD:197477
		PTZ00121	<355..739	CDD:173412
		SH3	766..825	CDD:388381
		INTAP	825..939	CDD:374674
		SH3_Intersectin1_2	939..990	CDD:212922
		SH3_Intersectin1_3	1028..1079	CDD:212924
		SH3_Intersectin1_4	1096..1160	CDD:212926
		SH3_Intersectin1_5	1180..1233	CDD:212928
		RhoGEF	1260..1443	CDD:238091	46/190 (24%)
		PH_13	1462..1615	CDD:374702	33/167 (20%)
		C2_Intersectin	1604..1738	CDD:176021	10/58 (17%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810