Protein Alignment RhoGEF4 and FGD5

DIOPT Version :10

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: XP_011531724.2
• Gene: FGD5 / 152273
• HGNCID: 19117
• Length: 1507
• Species: Homo sapiens
• Alignment Length: 546
• Identity: 118/546 - (21%)
• Similarity: 220/546 - (40%)
• Gaps: 106/546 - (19%)

  Fly    51 SNNSSPIGSPNPDAGKRLGFRRQAI---QEIISSEKSYLEQLE-LLMNFFVRPLKEQAIIDCSNH 111
            |:......|.:|....::..:.:|:   ||::||||:|:|.|: |.::|....::   .:|..:|
Human   913 SSEEEDSASRDPSVTHKVEGQSRALVIAQELLSSEKAYVEMLQHLNLDFHGAVMR---ALDDMDH 974

  Fly   112 ----TLL-------FGQIEMIHNLNGEFLRELEANMEN------VAHAFLKMAPFFKLYSVYAFD 159
                ||.       ..::..||:|:...|.|||..:.|      ||..||.....|..::.:...
Human   975 EGRDTLAREELRQGLSELPAIHDLHQGILEELEERLSNWESQQKVADVFLAREQGFDHHATHILQ 1039

  Fly   160 YRGALFIIQDLISKNP----VFRKFLEQTESRPEVQRKLNSLMIVPIQRVPRYKLLLEQVLLYTS 220
            :...|.::.:....:|    ..|:|.:..:...:..:  :.|:.| :||:.:|::||...|....
Human  1040 FDRYLGLLSENCLHSPRLAAAVREFEQSVQGGSQTAK--HRLLRV-VQRLFQYQVLLTDYLNNLC 1101

  Fly   221 PADADYKLLKESVKEIEATASHINTCVEEQEITQYLIHLQNSLVNRTPNIVKPSRRVIKEGVLQK 285
            |..|:|...:.::..|.......|..:|:.|..|.|:|:::| |....::::|.|..:|||.|.|
Human  1102 PDSAEYDNTQGALSLISKVTDRANDSMEQGENLQKLVHIEHS-VRGQGDLLQPGREFLKEGTLMK 1165

  Fly   286 ITHKGTEIKRYCVLMSDIFMYC---KMIKERAPNTVVENSLECCCIFPLKKCKVYEMLPGNFKLT 347
            :|.|... .|:..||:|:.:|.   |..|.|..||:...:::.       ...|.|.:|...|:.
Human  1166 VTGKNRR-PRHLFLMNDVLLYTYPQKDGKYRLKNTLAVANMKV-------SRPVMEKVPYALKIE 1222

  Fly   348 CQSDGIIFGSGDVQLSRTWVGFIRDAID----------LH--VQCRKTLRKDSSKR----TPIRK 396
            .....::..:........|.|.:..|:.          .|  |:.|:.|.....:|    .|:..
Human  1223 TSESCLMLSASSCAERDEWYGCLSRALPEDYKAQALAAFHHSVEIRERLGVSLGERPPTLVPVTH 1287

  Fly   397 KDM-KKFGADYVLSPNKRKCEY--DTVFRNKNRSTDSEEETED------SACFSRKRK---VASG 449
            ..| ...|.|:.|:..:..|..  ..|.||.:|:....:..:|      ..||...:|   ...|
Human  1288 VMMCMNCGCDFSLTLRRHHCHACGKIVCRNCSRNKYPLKYLKDRMAKVCDGCFGELKKRGRAVPG 1352

  Fly   450 ILRA---------PNGNPMGKASSSSSSSVSMKRVAPPPPAPPPPPAVQMRHQPGRPGELATASK 505
            ::|.         |..:|  :.|.|:.||| .:.:.|                       :|..|
Human  1353 LMRVTERPVSMSFPLSSP--RFSGSAFSSV-FQSINP-----------------------STFKK 1391

  Fly   506 ENRISKLYKKIAAGDKVANVRGILKR 531
            :.::.....::||..:.:.:.|.|.|
Human  1392 QKKVPSALTEVAASGEGSAISGYLSR 1417

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:459876	45/194 (23%)
		PH-like	269..>311	CDD:473070	15/44 (34%)
		PH	277..374	CDD:459697	23/99 (23%)
FGD5	XP_011531724.2	PHA03169	175..>366	CDD:223003
		RhoGEF	939..1125	CDD:459876	44/191 (23%)
		PH1_FGD5	1144..1266	CDD:275435	28/129 (22%)
		FYVE_FGD5	1280..1346	CDD:277281	13/65 (20%)
		PH2_FGD5_FGD6	1411..1501	CDD:270057	3/7 (43%)