Protein Alignment RhoGEF4 and FGD5

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: XP_011531724.1
• Gene: FGD5 / 152273
• HGNCID: 19117
• Length: 1464
• Species: Homo sapiens
• Alignment Length: 546
• Identity: 118/546 - (21%)
• Similarity: 220/546 - (40%)
• Gaps: 106/546 - (19%)

  Fly    51 SNNSSPIGSPNPDAGKRLGFRRQAI---QEIISSEKSYLEQLE-LLMNFFVRPLKEQAIIDCSNH 111
            |:......|.:|....::..:.:|:   ||::||||:|:|.|: |.::|....::   .:|..:|
Human   870 SSEEEDSASRDPSVTHKVEGQSRALVIAQELLSSEKAYVEMLQHLNLDFHGAVMR---ALDDMDH 931

  Fly   112 ----TLL-------FGQIEMIHNLNGEFLRELEANMEN------VAHAFLKMAPFFKLYSVYAFD 159
                ||.       ..::..||:|:...|.|||..:.|      ||..||.....|..::.:...
Human   932 EGRDTLAREELRQGLSELPAIHDLHQGILEELEERLSNWESQQKVADVFLAREQGFDHHATHILQ 996

  Fly   160 YRGALFIIQDLISKNP----VFRKFLEQTESRPEVQRKLNSLMIVPIQRVPRYKLLLEQVLLYTS 220
            :...|.::.:....:|    ..|:|.:..:...:..:  :.|:.| :||:.:|::||...|....
Human   997 FDRYLGLLSENCLHSPRLAAAVREFEQSVQGGSQTAK--HRLLRV-VQRLFQYQVLLTDYLNNLC 1058

  Fly   221 PADADYKLLKESVKEIEATASHINTCVEEQEITQYLIHLQNSLVNRTPNIVKPSRRVIKEGVLQK 285
            |..|:|...:.::..|.......|..:|:.|..|.|:|:::| |....::::|.|..:|||.|.|
Human  1059 PDSAEYDNTQGALSLISKVTDRANDSMEQGENLQKLVHIEHS-VRGQGDLLQPGREFLKEGTLMK 1122

  Fly   286 ITHKGTEIKRYCVLMSDIFMYC---KMIKERAPNTVVENSLECCCIFPLKKCKVYEMLPGNFKLT 347
            :|.|... .|:..||:|:.:|.   |..|.|..||:...:::.       ...|.|.:|...|:.
Human  1123 VTGKNRR-PRHLFLMNDVLLYTYPQKDGKYRLKNTLAVANMKV-------SRPVMEKVPYALKIE 1179

  Fly   348 CQSDGIIFGSGDVQLSRTWVGFIRDAID----------LH--VQCRKTLRKDSSKR----TPIRK 396
            .....::..:........|.|.:..|:.          .|  |:.|:.|.....:|    .|:..
Human  1180 TSESCLMLSASSCAERDEWYGCLSRALPEDYKAQALAAFHHSVEIRERLGVSLGERPPTLVPVTH 1244

  Fly   397 KDM-KKFGADYVLSPNKRKCEY--DTVFRNKNRSTDSEEETED------SACFSRKRK---VASG 449
            ..| ...|.|:.|:..:..|..  ..|.||.:|:....:..:|      ..||...:|   ...|
Human  1245 VMMCMNCGCDFSLTLRRHHCHACGKIVCRNCSRNKYPLKYLKDRMAKVCDGCFGELKKRGRAVPG 1309

  Fly   450 ILRA---------PNGNPMGKASSSSSSSVSMKRVAPPPPAPPPPPAVQMRHQPGRPGELATASK 505
            ::|.         |..:|  :.|.|:.||| .:.:.|                       :|..|
Human  1310 LMRVTERPVSMSFPLSSP--RFSGSAFSSV-FQSINP-----------------------STFKK 1348

  Fly   506 ENRISKLYKKIAAGDKVANVRGILKR 531
            :.::.....::||..:.:.:.|.|.|
Human  1349 QKKVPSALTEVAASGEGSAISGYLSR 1374

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	45/194 (23%)
		PH-like	269..>311	CDD:302622	15/44 (34%)
		PH	277..374	CDD:278594	23/99 (23%)
FGD5	XP_011531724.1	RhoGEF	896..1083	CDD:279015	44/192 (23%)
		PH1_FGD5	1101..1223	CDD:275435	28/129 (22%)
		PH	1116..1207	CDD:278594	24/98 (24%)
		FYVE_FGD5	1237..1303	CDD:277281	13/65 (20%)
		PH	1368..1462	CDD:278594	3/7 (43%)
		PH2_FGD5_FGD6	1368..1458	CDD:270057	3/7 (43%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X316
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.910