Protein Alignment RhoGEF4 and Fgd6

DIOPT Version :10

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: XP_036011484.1
• Gene: Fgd6 / 13998
• MGIID: 1261419
• Length: 1401
• Species: Mus musculus
• Alignment Length: 568
• Identity: 124/568 - (21%)
• Similarity: 217/568 - (38%)
• Gaps: 153/568 - (26%)

  Fly    50 DSNNSSPIGSPNP-------DAGKRLGFRRQAIQEIISSEKSYLEQLELL-MNF----------F 96
            |.::.|..|.|:|       |||.:......| :||:||||.:::.|:|| ::|          .
Mouse   815 DVSSESSKGEPDPLEDKQDEDAGMKSKVHHIA-KEIMSSEKVFVDVLKLLHIDFRGAVAHASRQL 878

  Fly    97 VRPLKEQAIIDCSNHTLLFGQIEMIHNLNGEFLRELEANM------ENVAHAFLKMAPFFKLYSV 155
            .:|:.|..|:   |..|.:  :..::.||.:.|:|||..|      :.:|..|:|..|:.|:||.
Mouse   879 GKPVIEDRIL---NQILYY--LPQLYELNRDLLKELEERMLTWTEQQRIADIFVKKGPYLKMYST 938

  Fly   156 YAFDYRGALFIIQDLISKNPVFRKFLEQTESRPE-VQRKLNSLMIVPIQRVPRYKLLLEQVLLYT 219
            |..::...:.::.:...|||.|...:.:.|..|. ....|...::.|:||:|:|:|||...|...
Mouse   939 YIKEFDKNVALLDEQCKKNPGFAAVVREFEMSPRCANLALKHYLLKPVQRIPQYRLLLTDYLKNL 1003

  Fly   220 SPADADYKLLKESVKEIEATASHINTCVEEQEITQYLIHLQNSLVNRTPNIVKPSRR--VIKEGV 282
            .....|::..::::..:...|:|.|..:::.:..|.|:.:|.||... ..||:|.|.  .:|||.
Mouse  1004 LEDSVDHRDTQDALAVVIEVANHANDTMKQGDNFQKLMQIQYSLSGH-HEIVQPGRNDVFLKEGT 1067

  Fly   283 LQKITHKGTEIKRYCVLMSDIFMYCK-----MIKERAPNTVVENSLECCCIFPLKKCKV----YE 338
            |.|::.|..: .|...|.:|..:|..     |.|       :.|.|.      |...||    .|
Mouse  1068 LMKLSRKVMQ-PRMFFLFNDALLYTTPMQSGMYK-------LNNMLS------LAGMKVRKPTQE 1118

  Fly   339 MLPGNFKLTCQSDGIIFGSGDVQLSRTWVGFIRDAIDLHVQCR------KTLRKDSSKR---TPI 394
            ......|:.......|..:........|:..|..:|:.:.:.|      ::|.:||.::   :|:
Mouse  1119 AYQNELKIESVERSFILSASSAAERDDWLEAISSSIEEYAKKRITFCPSRSLDEDSERKEEVSPL 1183

  Fly   395 RKK----------------------------------------DMKKFGADYVLSPNKRKCEYDT 419
            ..|                                        ...|:|.||:.....|.||:
Mouse  1184 GAKAPIWIPDTRATMCMICTSEFTLTWRRHHCRACGKIVCQACSSNKYGLDYLKGQLARVCEH-- 1246

  Fly   420 VFRNKNRSTDSEEETEDSACFSRKRKVASGILRAPNGNPMGKASSSSSSSVSMKRVAPPPPAPPP 484
                               ||...:|:...:  :|.....|...|.||:..|:....|
Mouse  1247 -------------------CFQELQKLDHQL--SPRVGSPGNHKSPSSALSSVLHSIP------- 1283

  Fly   485 PPAVQMRHQPGRPGELATASKENRISKLYKKIAAGDKVANVRGILKRS 532
                     .||        |:.:|....|:::|..:.:.:.|.|.||
Mouse  1284 ---------SGR--------KQKKIPAALKEVSANTEDSTMSGYLYRS 1314

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:459876	50/187 (27%)
		PH-like	269..>311	CDD:473070	15/48 (31%)
		PH	277..374	CDD:459697	22/105 (21%)
Fgd6	XP_036011484.1	RhoGEF	845..1028	CDD:459876	50/188 (27%)
		PH-like	1047..1171	CDD:473070	29/138 (21%)
		FYVE_FGD6	1188..1248	CDD:277282	7/80 (9%)
		PH2_FGD5_FGD6	1307..1395	CDD:270057	4/8 (50%)