DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF4 and FGD4

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	NP_001357227.2	Gene:	FGD4 / 121512	HGNCID:	19125	Length:	903	Species:	Homo sapiens

Alignment Length:	508	Identity:	119/508 - (23%)
Similarity:	211/508 - (41%)	Gaps:	106/508 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly    31 LQERNLLTAKSRRKVCSMLDSNNSSPIGSPNPDAGKRLGFRRQ-----AIQEIISSEKSYLEQLE 90
            |:||.   |::..||...  .|..||:.....|....:....:     ...|::.:|::|:.:|:
Human   304 LEERG---AETETKVQER--ENGESPLELEQLDQHHEMKETNEQKLHKIANELLLTERAYVNRLD 363

  Fly    91 LLMNFFVRPLKEQA---IIDCSNHTLLFGQIEMIHNLNGEF-LRELEANME------NVAHAFLK 145
            ||...|...|.|:|   .........:|..|..|:..:.:| |.|||..|:      .:.....|
Human   364 LLDQVFYCKLLEEANRGSFPAEMVNKIFSNISSINAFHSKFLLPELEKRMQEWETTPRIGDILQK 428

  Fly   146 MAPFFKLYSVYAFDYRGALFIIQDLISKNPVFRKFLEQTESRPEVQRK-------LNSLMIVPIQ 203
            :|||.|:|..|...:..|:.:::::..:.|.|:..:|      |:|::       |...|:.|:|
Human   429 LAPFLKMYGEYVKGFDNAMELVKNMTERIPQFKSVVE------EIQKQKICGSLTLQHHMLEPVQ 487

  Fly   204 RVPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATASHINTCVEEQEITQYLIHLQNSLVNRTP 268
            |:|||::||:..|....|...|:...|:|::.|...|||.|:.:.:.|..:.|:.:. .::....
Human   488 RIPRYEMLLKDYLRKLPPDSLDWNDAKKSLEIISTAASHSNSAIRKMENLKKLLEIY-EMLGEEE 551

  Fly   269 NIVKPSRRVIKEGVLQKITHKGTEI-KRYCVLMSDIFMYCKMIKERAPN-TVVENSLECCCIFPL 331
            :||.||..:||||.:.|:..:.|.. :||..|.:::.:||      .|. ::|.:.........:
Human   552 DIVNPSNELIKEGQILKLAARNTSAQERYLFLFNNMLLYC------VPKFSLVGSKFTVRTRVGI 610

  Fly   332 KKCKVYEM----LPGNFKLTCQSDGIIFGSGDVQLSRTWVGFIRDAIDL----HVQCRKTLRKDS 388
            ...|:.|.    .|..|:::.:...:...:...|....|:..:::.||.    |...|..:.||:
Human   611 DGMKIVETQNEEYPHTFQVSGKERTLELQASSAQDKEEWIKALQETIDAFHQRHETFRNAIAKDN 675

  Fly   389 -----------SKRTP--IRKKDM-------KKFGA-----------DYVL----SPNKRKCEYD 418
                       .||.|  ||..::       :.|.|           .||:    |..|.:.|||
Human   676 DIHSEVSTAELGKRAPRWIRDNEVTMCMKCKEPFNALTRRRHHCRACGYVVCWKCSDYKAQLEYD 740

  Fly   419 ---------TVFRNKNRSTDSEEETEDSACFSRKRKVASGILRAPNGNPMGKA 462
                     ..::..:..|||||         :|||   |||...:....|.:
Human   741 GGKLSKVCKDCYQIISGFTDSEE---------KKRK---GILEIESAEVSGNS 781

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	51/186 (27%)
		PH-like	269..>311	CDD:302622	15/42 (36%)
		PH	277..374	CDD:278594	19/102 (19%)
FGD4	NP_001357227.2	RhoGEF	344..528	CDD:238091	51/189 (27%)
		PH1_FDG4	560..653	CDD:275434	19/98 (19%)
		FYVE_FGD1_2_4	691..755	CDD:277280	12/63 (19%)
		PH2_FGD1-4	774..876	CDD:270056	1/8 (13%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		oto91241
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R7163
SonicParanoid	1	1.000	-	-		X316
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	6	5.800