DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment RhoGEF4 and akap13

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	XP_021327529.1	Gene:	akap13 / 108190639	-ID:	-	Length:	1416	Species:	Danio rerio

Alignment Length:	707	Identity:	127/707 - (17%)
Similarity:	263/707 - (37%)	Gaps:	158/707 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly    20 PLSFSRELRQ---VLQERNLLTAKSR------RKVCSMLDSNNSSPIGSPNPDA----------- 64
            |||..|.|.|   .|.:.|.:||.:.      ..|.|.|..:..:.:.....|:
Zfish   552 PLSGRRYLSQSTDSLHKPNKVTASNESLDEGTEMVDSRLMGDFEADVKELEADSWSLIVDKKYLK 616

  Fly    65 --GKRLGFRRQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHTLLFGQIEMIHNLNGE 127
              .|.:..|:..|.|:|.:|..:|..|.::.:.:.:.|.....::......:|..::.:..|:..
Zfish   617 QLKKDIIKRQDVIYELIQTEMHHLRTLRIMCDVYSKGLLMDLQLEPQAVEKMFPMLDDLLELHSF 681

  Fly   128 FL-----RELEANMEN----VAHAF--LKMAPFF--------KLYSVYAFDYRGALFIIQDLISK 173
            |.     |:.||.::.    :.|..  |.::.|.        ::|..:...:..|:...:||.::
Zfish   682 FFSALLERKKEARLDGTDGFLIHRIGDLLVSQFSDSNAESMKRIYGKFCSRHNEAVNFYKDLHAR 746

  Fly   174 NPVFRKFLEQTESRPEVQR-KLNSLMIVPIQRVPRYKLLLEQVLLYTSPADADYKLLKESVKEIE 237
            :..|:.|:.:..|...|:| .:...:::..||:.:|.:||:::|.:|...:.|::.|.::.:.::
Zfish   747 DKRFQAFIRKKMSSSVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEKDFEDLTQAFQLLK 811

  Fly   238 ATASHINTCVEEQEITQYLIHLQNSLVNRTPNIVKP-----------SRRVIKEGVLQKITHKGT 291
            ...:.:::.|.|.|..:.|..:.....:::...:|.           .||::.:|.||.....|.
Zfish   812 DIIASVDSKVNEHEKKKKLKEIYGRTDSKSITRMKSGQMFAREDLVRGRRLLHDGPLQLKNTAGR 876

  Fly   292 EIKRYCVLMSDIFMYCKMIKERAPNTVVENSLECCCIFPLKKCKVYEML---PGNFKLT---CQS 350
            ....:.:|:||:.::   ::|:....|..:..:...:..|:|..|.|:.   .|.|.:|   ...
Zfish   877 LKDVHALLLSDVLVF---LQEKDQKYVFASLDQRATVISLQKLIVREVAHEERGLFLITAGIANP 938

  Fly   351 DGIIFGSGDVQLSRTWVGFIRDAIDLHVQCRKTLRKDSSKRTPIRKKDMKKFGADYVLSPNKRKC 415
            :.:...:...:...||:..|:||  :|     ::.||..:..|...::.:|.
Zfish   939 EMVEVHASSREERNTWMQIIQDA--MH-----SIEKDDDEGIPSETEEDRKL------------- 983

  Fly   416 EYDTVFRNKNRSTDSEEETEDSACFSRKRKVASGILRAPNGNPMGKASSSSSSSVSMKRVAPPPP 480
             .:|..|........::|...|....:.:     |.|...|       ||....|.|
Zfish   984 -LETKTREMREMLQRKDEQIVSLLLEKMK-----IFREMCG-------SSEDPQVKM-------- 1027

  Fly   481 APPPPPAVQMRHQPGRPGELATASKENRISKLYKKI------AAGDKVANVRGILKRSNVPAPNN 539
                  ..:..::....||.........:..|...:      |.|.:||..:||:....:|
Zfish  1028 ------LFRANNEEVAKGEPIMTDALREVEMLQALVNSSLGGAVGQQVACAQGIMGPVCLP---- 1082

  Fly   540 DHDPSYGFASRYSDSKSYFRAHNVDNTIPTFVKR--EDLFNGD-----------NMFPVHLKSHE 591
                      |.:::...|..|.: ||.....|.  |||...:           |:..:..|:.|
Zfish  1083 ----------RRAETFGGFDCHQM-NTCKHGEKEEAEDLRRTESDSVLKKGGNANLLLLLKKNSE 1136

  Fly   592 EICTPPQKKRVKFDDTLDAVSPVLVEKPFAFSSESSSAL----------KPTSLRER 638
            ::.|    ......|.|.::..|::::. .|..:...||          :|.||.|:
Zfish  1137 QVLT----SVTHLHDLLSSLQAVVLQQD-TFIEDQRQALSERTPSRPSSRPPSLVEQ 1188

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	34/189 (18%)
		PH-like	269..>311	CDD:302622	10/52 (19%)
		PH	277..374	CDD:278594	20/102 (20%)
akap13	XP_021327529.1	C1	428..472	CDD:197519
		RhoGEF	625..818	CDD:238091	35/192 (18%)
		PH_AKAP13	862..964	CDD:275427	22/111 (20%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.900