Protein Alignment RhoGEF4 and Arhgef18

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: XP_030099069.1
• Gene: Arhgef18 / 102098
• MGIID: 2142567
• Length: 1434
• Species: Mus musculus
• Alignment Length: 591
• Identity: 105/591 - (17%)
• Similarity: 202/591 - (34%)
• Gaps: 177/591 - (29%)

  Fly    71 RRQAIQEIISSEKSYLEQLELLMNFFVRPLKEQAIIDCSNHTLLFGQIEMIHNLNGEFLRELEAN 135
            |:..:.|::.:|..::..|::::..:.|.|:|:........:.||...:.:.:::..||..|:..
Mouse   515 RQDVLYELMQTEAHHVRTLKIMLKVYSRALQEELQFSGQAVSRLFPCADDLLDMHSHFLARLKER 579

  Fly   136 MENVA-------HAFLKMAPFF-------------KLYSVYAFDYRGALFIIQDLISKNPVFRKF 180
            .:...       :...|:....             :.|:|:...:..|:...:.|:.::..|:..
Mouse   580 RQEFLEEGSDRNYVIQKIGDVLVQQFSGETGERMKEKYAVFCSGHNDAVGQYKLLLQQSKKFQNL 644

  Fly   181 LEQTESRPEVQR-KLNSLMIVPIQRVPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATASHIN 244
            :::..:...|:| .:...:::..||:.:|.:|:|:::..|.....|||.|.:::..|:...|.::
Mouse   645 IKKIGNFSIVRRLGVQECILLVTQRITKYPVLVERIIQNTEAGTEDYKDLSQALSLIKDIISQVD 709

  Fly   245 TCVEEQEITQYLIH------------LQNSLVNRTPNIVKPSRRVIKEGVLQKITHKGTEIKRYC 297
            ..|.|.|..|.|..            |:|.|..|..:::: .|::..||.|...:..|.......
Mouse   710 AKVSEYEKDQRLKEIAAKTDQKSSGKLKNGLTFRKEDMLQ-QRQLHLEGALCWKSTSGRLKDVLA 773

  Fly   298 VLMSDIFMYC----------------------KMIKERAPN------------------------ 316
            ||::|:.:..                      |:|.....|
Mouse   774 VLLTDVLLLLQEKDQKYVFASVDSKPPVISLQKLIVREVANEEKAMFLISASMQGPEMYEMYTSS 838

  Fly   317 --------TVVENSLECC-----CIFP---------------------------------LKKCK 335
                    ..:..::|.|     .:|.                                 |:|.:
Mouse   839 KEDRNIWMAHIRRAVESCPDEEEDVFSEAEEKKIAEARTMKLQEFQERLSLKDQLIAQSLLEKQQ 903

  Fly   336 VY-EM--LPGNFKLTCQSDGIIFGSGDVQLSRTWVGFIRDAID-----LHVQCRKTLRKDSSK-- 390
            :| ||  |.| .:.:.|:.|:..|.||...:......:|.|:.     ..:.|::.|...||:
Mouse   904 IYLEMAQLSG-LEESAQNRGLFRGGGDPSETLRGEQILRSAMSEIEGIQSLICQRHLGSTSSQVE 967

  Fly   391 ------RTPIRKKDMKKFGADYVLSPNKRKCEYDTVFRNKNRSTDSEEETEDSACFSRKRKVASG 449
                  ..|.|.:...  |.|.|.||:|..                          |.||||::.
Mouse   968 EGSVSAGLPRRAETFG--GYDSVGSPSKGG--------------------------SFKRKVSNS 1004

  Fly   450 ILRAPNGNPMGKASSSSSSSVSMKRVAPPPPAPPPPPAVQMRHQPGRPG--ELATASKENRISKL 512
            .||..:.  .|.|||..|....  ..||.......|.||:|......|.  ||....:...:|:|
Mouse  1005 DLRPQDW--QGPASSPDSRPCD--NSAPSGCCEESPQAVEMPSTESLPTVLELELVHRVQTLSQL 1065

  Fly   513 YKKIAA 518
            ...:.|
Mouse  1066 LLSLQA 1071

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	31/190 (16%)
		PH-like	269..>311	CDD:302622	9/63 (14%)
		PH	277..374	CDD:278594	26/191 (14%)
Arhgef18	XP_030099069.1	RhoGEF	515..709	CDD:238091	32/193 (17%)
		PH_ARHGEF18	750..868	CDD:275437	14/117 (12%)
		DUF5401	<1080..>1197	CDD:375164
		PRK13729	1153..>1305	CDD:184281

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810