DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF4 and fgd5

DIOPT Version :9

Sequence 1:	NP_648101.1	Gene:	RhoGEF4 / 38806	FlyBaseID:	FBgn0035761	Length:	647	Species:	Drosophila melanogaster
Sequence 2:	XP_002938283.3	Gene:	fgd5 / 100496480	XenbaseID:	XB-GENE-961644	Length:	1560	Species:	Xenopus tropicalis

Alignment Length:	688	Identity:	139/688 - (20%)
Similarity:	251/688 - (36%)	Gaps:	176/688 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 PRQKGYGNGFIHSPQTPLSFSR--------------ELRQVLQERNLLTAKSRRKVCSMLDSNNS 54
            |.:.|....|...|...||.:.              |::|...:..........|||.:|.|...
Frog   908 PARAGTFTEFFEDPSRALSSANENDGYVDMSSFNTFEIKQAADQEAESAYTEPYKVCPILGSMEE 972

  Fly    55 SPIGSPNP----DAGKRLG----------FRRQAIQE-IISSEKSYLEQLELL-MNFFVRPLK-- 101
            .......|    :.|..||          .|...|.| ::||||.|::.|:.| :.|....||
Frog   973 DITSDEEPKSSEEEGNCLGESGVVQKNGPMRALTIVESLLSSEKEYVDTLKRLHIEFHTAVLKVL 1037

  Fly   102 -------EQAIIDCSNHTLLFGQIEM--IHNLNGEFLRELEANM------ENVAHAFLKMAPFFK 151
                   |..:      .|..|..|:  |:.::.|.|.|||..:      :.:|..||.....|:
Frog  1038 GSEEEDGEDEL------KLRHGLSELLNIYLIHEEILAELEQRITHWEESQKIADIFLSRESDFQ 1096

  Fly   152 LYSVY--AFDYRGALF------------IIQDLISKNPVFRKFLEQTESRPEVQRKLNSLMIVPI 202
            .::.:  |||...|..            :::|       |.::|.:.::  .|:.:|    :..:
Frog  1097 HHTAFIVAFDKMTAALDDCTLKISHLATVVRD-------FERYLHRGQA--NVKHEL----LKVV 1148

  Fly   203 QRVPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATASHINTCVEEQEITQYLIHLQNSLVNRT 267
            ||:.:|.::|...|....|...:|:....::..:...|...|..:.:.|..|.|:.:::| |...
Frog  1149 QRIFQYHMILTDYLNNLCPDWTEYEDTHAALLMVSEVADRANDSMRQGENLQKLVQIEHS-VRGQ 1212

  Fly   268 PNIVKPSRRVIKEGVLQKITHKGTEIKRYCVLMSDIFMYCKMIKE---RAPNT-----------V 318
            .::::|.|..:|||.|.|::.:... .|:..||:|:.:|...:|:   |..||           :
Frog  1213 RSLLQPGREFVKEGTLMKVSGRNRH-PRHLFLMNDVLLYTYPLKDGKYRLKNTLSIPGMRVSRPI 1276

  Fly   319 VENS-----LEC---CCIFPLKKCKVYEMLPGNFKLTCQSDGIIFGSGDVQLSRTWVGFIRD--- 372
            |||:     :||   |.:.....|...:..     .:|             :|||...:.:.
Frog  1277 VENAQNVLKVECMERCLMLSASSCTERDEW-----YSC-------------ISRTVQEYYKAPTV 1323

  Fly   373 AIDLHVQCRKTLRKDSSKRTP-----IRKKDMKKFGADYVLSPNKRKCEY--DTVFRNKNRSTDS 430
            :|..:::.|:.|.....:|.|     .........|:|:.|:..:..|..  ..:.|:.:|:...
Frog  1324 SIYNNLEIRERLGMGLGERPPSVAHMSHAVMCMNCGSDFTLTLRRHHCHACGKIICRSCSRNKYP 1388

  Fly   431 EEETED------SACFSRKRKVASGILRAPNGNPMGKASSSSSSSVSMKRVAPPPPAPPPPPAVQ 489
            .:..:|      :.||:..||.....:...:..|:.:::|:.|:..|...           |:..
Frog  1389 LKYLKDRPSKVCNGCFAELRKRDLAAMNMNSSPPLPRSTSTLSAMFSSIH-----------PSYF 1442

  Fly   490 MRHQPGRPGEL--ATASKEN--------RISK----------------LYKKIAAGDKVANVRGI 528
            .||:. .|..|  |.||.||        |..|                ||...|..||:|.....
Frog  1443 RRHKK-IPSALMEAAASGENASISGYLHRCKKGKKYWKKRWFVIKGKVLYTYTACEDKIATESLP 1506

  Fly   529 LKRSNVPAPNNDHDPSYGFASRYSDSKSYFRAHNVDNT 566
            |...::...|.:.:|..|...:....|:.|.:....:|
Frog  1507 LLGFHIVPDNWNENPKLGTVFQLYHKKTLFYSFKAADT 1544

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	43/202 (21%)
		PH-like	269..>311	CDD:302622	12/41 (29%)
		PH	277..374	CDD:278594	25/121 (21%)
fgd5	XP_002938283.3	MSCRAMM_ClfB	<49..205	CDD:411414
		RhoGEF	1007..1190	CDD:395496	43/201 (21%)
		PH1_FGD5	1209..1331	CDD:275435	29/140 (21%)
		FYVE_FGD5	1344..1410	CDD:277281	11/65 (17%)
		PH-like	1464..1554	CDD:418428	15/81 (19%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm49235
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X316
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	2.000