Protein Alignment RhoGEF4 and fgd5a

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: XP_001919894.2
• Gene: fgd5a / 100151562
• ZFINID: ZDB-GENE-090428-3
• Length: 1473
• Species: Danio rerio
• Alignment Length: 567
• Identity: 117/567 - (20%)
• Similarity: 206/567 - (36%)
• Gaps: 110/567 - (19%)

  Fly    76 QEIISSEKSYLEQLELLMNFFVR----------PLKEQAIIDCSNHTLLFGQIEMIHNLNGEFLR 130
            ::::.||:.:::.|::|...|..          ||.|...:|     .:|..:...::|:.|.|.
Zfish   921 KDLVDSEREHVKALKVLQEDFRAAVESGGDDNGPLIENGKLD-----EIFSTLPQAYSLHSEILN 980

  Fly   131 ELEANME------NVAHAFLKMAPFFKLYSVYAFDYRGALFIIQDLISKNPVFRKFLEQ--TESR 187
            |||..::      .|....|.....|..::.|..:|..::.::.:...|:..|...::|  |::.
Zfish   981 ELETRIKQWEDSPTVVDVILTRRAEFSSFATYISNYDRSMSVLDESCRKSQAFEDVVKQFETQNA 1045

  Fly   188 PEVQRKLNSLMIVPIQRVPRYKLLLEQVLLYTSPADADYKLLKESVKEIEATASHINTCVEEQEI 252
            ..|...|...::..|.|:.:|::||...|...||...:|:..:.::..:...|..:|..::..|.
Zfish  1046 EGVLVPLKHQLLRVIVRILQYRMLLTDYLNNLSPDSKEYEDTQAALVIVSEVADQVNDNLKHGEN 1110

  Fly   253 TQYLIHLQNSLVNRTPNIVKPSRRVIKEGVLQKITHKGTEIKRYCVLMSDIFMYC---KMIKERA 314
            ...|:|:..| |....::::|.|..:|||.|.|::.|..: .|:..||:|:.:|.   :..|.|.
Zfish  1111 LLRLVHIDYS-VRGKRDLLQPGRVFVKEGTLMKVSRKSRQ-PRHLFLMNDVMLYTYPQQDGKYRL 1173

  Fly   315 PNTVVENSLECCCIFPLKKCKVYEMLPGNFKLTCQ---SD-GIIFGSGDVQLSRTWVGFIRDAID 375
            .||:.           |...||.:.:..|...|.:   || .|...:..:.....|...:..||.
Zfish  1174 KNTLT-----------LSGMKVSKPIIDNVLNTLRIEVSDVTITLSASSLGEREDWFHTLSRAIA 1227

  Fly   376 LHVQCRKTLRKDSSKR--------------TPIRKKDM-KKFGADYVLSPNKRKCEY--DTVFRN 423
            .|.....|....|..|              .|:....| ....:|:.|:..:..|..  ..|.|:
Zfish  1228 DHAAGLNTFSSSSEAREKLWMSLGEAAPVLVPVSHVMMCMNCTSDFSLTLRRHHCNACGKVVCRS 1292

  Fly   424 KNRSTDSEEETEDSACFSRKRKVAS---GILRAPNGNPMGKASSSSSSSVSMKRVAPPPPAPPPP 485
            .:|:....:..:|     |..||..   ..||...|:..|...|.|..:....|..........|
Zfish  1293 CSRNRYPLKYLKD-----RMAKVCDHCYAELRKRGGDVPGSCGSVSPRTHRASRPLSAVFQSLQP 1352

  Fly   486 PAVQMRHQPGRP--------------GELATASKENRISK----------LYKKIAAGDKVANVR 526
            |::....:...|              |.|....|..|..|          ||...|..||||:..
Zfish  1353 PSLWRNRKSSSPLTQVSVGAEGSTMSGSLQRRKKSKRRWKRLWFLLKDKVLYTFKAREDKVASES 1417

  Fly   527 GILKRSNVPAPNNDHDPSYGFASRYSDSKSYFRAHNVDNTIPTFVKR 573
            ..|:               ||..:.||..   ...:.||....:.|:
Zfish  1418 LSLQ---------------GFTVKLSDRS---EGEDTDNVFQLYHKK 1446

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	37/185 (20%)
		PH-like	269..>311	CDD:302622	13/44 (30%)
		PH	277..374	CDD:278594	24/103 (23%)
fgd5a	XP_001919894.2	FYVE_FGD5	1255..1321	CDD:277281	14/70 (20%)
		PH2_FGD5_FGD6	1377..1467	CDD:270057	20/88 (23%)
		DnaJ	<25..149	CDD:333066
		RhoGEF	919..1103	CDD:214619	37/186 (20%)
		PH1_FGD5	1121..1243	CDD:275435	32/133 (24%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X316
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.910