Protein Alignment RhoGEF4 and fgd4

DIOPT Version :9

• Sequence 1: NP_648101.1
• Gene: RhoGEF4 / 38806
• FlyBaseID: FBgn0035761
• Length: 647
• Species: Drosophila melanogaster
• Sequence 2: XP_031754365.1
• Gene: fgd4 / 100038050
• XenbaseID: XB-GENE-493422
• Length: 895
• Species: Xenopus tropicalis
• Alignment Length: 495
• Identity: 123/495 - (24%)
• Similarity: 209/495 - (42%)
• Gaps: 111/495 - (22%)

  Fly    54 SSPIGSPNPDAG-------KRLGFRRQAIQ-------EIISSEKSYLEQLELLMNFFVRPLKE-- 102
            |||.|....|.|       :...|:....|       |::.:|::|:.:||||..|....:||
 Frog   308 SSPTGEALEDIGTINITIAEPTDFKETNEQKLHNIANELLETERAYVSRLELLQTFHDALMKEAK 372

  Fly   103 QAIIDCSNHTLLFGQIEMIHNLNGEF-LRELEANME------NVAHAFLKMAPFFKLYSVYAFDY 160
            |..........:|..|..|.:.:|:| |.|||:.|:      .:.....|:|||.|:|:.|..::
 Frog   373 QGSFPVEVLNKIFSNISSIQSFHGQFLLPELESRMKEWSISPKIGDILQKLAPFLKMYAEYVKNF 437

  Fly   161 RGALFIIQDLISKNPVFRKFLEQTESRPEVQRK-------LNSLMIVPIQRVPRYKLLLEQVLLY 218
            ..|:..::..:.|:..|:..:|      |:||:       |...|:.|:||:|||::||:. .|.
 Frog   438 DNAMETLRGWMEKSVQFKNVVE------EIQREGKCGNLTLQHHMLGPVQRIPRYEMLLKD-YLR 495

  Fly   219 TSPADA-DYKLLKESVKEIEATASHINTCVEEQEITQYLIHLQNSLVNRTPNIVKPSRRVIKEGV 282
            ..|||: |.|..:::::.|...|:|.||.:.:.|..:.|:.:. .::....:||.||..:||||.
 Frog   496 KLPADSLDRKDAEKALELISFAATHSNTAIRKMENLKKLLSIY-EMLGEEEDIVHPSNELIKEGQ 559

  Fly   283 LQKITHKGTEI-KRYCVLMSDIFMYCKMIKERAPN-TVVENSLECCCIFPLKKCKVYEM----LP 341
            :.|:..:.|.. :||..|::::.:||      .|. ::|...........|:..||.|.    .|
 Frog   560 ILKLAARNTSAQERYLFLLNNMLLYC------VPKFSLVGTKYTLRTKIGLEGMKVVETQNEDYP 618

  Fly   342 GNFKLTCQSDGIIFGSGDVQLSRTWVGFIRDAI--------DLHVQCRKTLRKDSSK-------- 390
            ..|:::.:...:...:...|....|:..:||.|        ...:...|.|.:..|:
 Frog   619 HTFQISGKERTLELQASSEQNKEEWIKALRDTIAEVQQKNETFKIAIAKELEETPSEVSRAELGL 683

  Fly   391 RTP--IRKKDM-------KKFGA-----------DYVL----SPNKRKCEYDTVFRNK------- 424
            |.|  ||..::       ::|.|           .||:    |..|...|||:...||
 Frog   684 RAPRWIRDNEVTMCMKCKEQFNALTRRRHHCRACGYVVCWKCSDYKATLEYDSNKMNKVCKDCYK 748

  Fly   425 --NRSTDSEEETEDSACFSRKRKVASGILRAPNGNPMGKA 462
              ..|.||||:       .:||    |||...:....|.:
 Frog   749 ILRGSIDSEEK-------EKKR----GILEIESAEVSGNS 777

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF4	NP_648101.1	RhoGEF	74..244	CDD:279015	55/193 (28%)
		PH-like	269..>311	CDD:302622	15/42 (36%)
		PH	277..374	CDD:278594	23/102 (23%)
fgd4	XP_031754365.1	PspC_subgroup_2	<85..349	CDD:411408	9/40 (23%)
		RhoGEF	339..522	CDD:238091	54/189 (29%)
		PH1_FDG4	554..647	CDD:275434	21/98 (21%)
		FYVE_FGD1_2_4	686..750	CDD:277280	14/63 (22%)
		PH2_FGD1-4	770..872	CDD:270056	1/8 (13%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm49235
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X316
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	2.000