DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Dscam2 and robo4

DIOPT Version :9

Sequence 1:	NP_001261500.1	Gene:	Dscam2 / 38788	FlyBaseID:	FBgn0265296	Length:	2101	Species:	Drosophila melanogaster
Sequence 2:	XP_689255.3	Gene:	robo4 / 560765	ZFINID:	ZDB-GENE-020809-1	Length:	1134	Species:	Danio rerio

Alignment Length:	1083	Identity:	237/1083 - (21%)
Similarity:	393/1083 - (36%)	Gaps:	268/1083 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly   561 PDD-IRQRVQPDGSLTISPVQKNSDSGVYTCWARNKQGHSARRSGEVTVIVPPKLSPFQTNILQL 624
            ||: :.||.:..|.|....:.:.:        :...:.|..:.|..|:.|..|::....:::: :
Zfish    27 PDEQVAQRSEGKGHLRHRLMHQRA--------SHRDRAHRRKGSRLVSEINLPRIVHHPSDVV-V 82

  Fly   625 NMGDRASLTCSVVKGDLPLTINWRKDGRPIDPTQHMSVKQVDQYNSILVIEN------------L 677
            .:|..|:|:|. .:|:...||.|.::|:|:|      ..::|..:..:|:.:            .
Zfish    83 RVGSPATLSCR-AEGNPEPTIQWLRNGQPLD------TDKMDAQSQPIVLPDGSLFFFSVVPGRK 140

  Fly   678 GSDHTGNYSCVVRNSA--AEVENSQALLVNVPPRWIVEPVDANVERNRHIMLHCQAQ-GVPTPSI 739
            |..|...|:|:..||.  |...|:...:..:...:.|:|.|..|.......::|... |.|.|::
Zfish   141 GQSHEAVYACIAHNSIGNATSRNASLHIAALREDFRVQPSDVEVAIGEMATINCSPPVGHPEPNV 205

  Fly   740 VWKK---ATGSKSGEYEEVRERPFTKLLGNGSLLLQHVKEDREGFYLCQANNGIGTGIGKVIQLK 801
            .|:|   ...|.:..|.|::          |.|::...:::..|.|.|.|:|.||....:..:|.
Zfish   206 TWRKDGILINSSNEHYTELK----------GKLIIAPAQKNDSGVYSCIASNMIGVRESRAARLS 260

  Fly   802 VNSSPYFSSTSRSVMVKKGDTALLQCAVSGDKPINIVWMR------SGKNTLNPSTNYKISVKQE 860
            |.:.|........|.|:.|::|...|...||...:|.|.|      :|:..:||..:
Zfish   261 VLAKPVLLRKPEDVSVQLGESAQFFCEADGDPMPSIEWSREQGPLPNGRYLINPDHS-------- 317

  Fly   861 ATPDGVSAELQIRTVDATDSGPYFCRASNLYGNDQQLVQLQVQE-------------PPLPPSVL 912
                     |||..|.|.|.|.|.|...|..|......||.|::             ..|..|:.
Zfish   318 ---------LQIHYVTAQDMGRYSCTVENKLGVSVASAQLLVEDAGGTRLRDLHKELSALRVSLE 373

  Fly   913 EAAMISSRS--VNIKWQPKTLGTGDVTKYIVEFREADHSLPPALFVDQWQQIEVKDPPHFNAMIE 975
            ...::|:.|  ..:.|:.::.|:  ...|:..|.....||.||  ...|....|.. |..:..:.
Zfish   374 NVTVMSTASNMSQVMWKLQSFGS--QPHYLEGFEVLYRSLLPA--SSDWTAQRVPQ-PSLHTHVG 433

  Fly   976 NLKPATRYAFRVIAEGSA--GRSAPSQELIVRTEPQRPAGPPLSLS-ARPLSSTELL-ISWVAPL 1036
            .||...:|.|:|...||:  ||.:.::.|  |.....|:..|..:| ..|....:.: :||..|.
Zfish   434 PLKRGYKYEFKVRPYGSSLYGRESNTRHL--RVPEIVPSAAPQDVSITMPAERNDTVHLSWEPPP 496

  Fly  1037 PELRHGDIQGYNVGYKLSSSGNTAYNFTSVSGDGDGGNGELLLSGLAKFARYTVVVQAFNQVGPG 1101
            .:..:|.||||.| :.:.|...|::|:|.     |.|...:.:..|.....|.|.|.|.|..|.|
Zfish   497 HDAHNGIIQGYQV-WCVESEEQTSFNWTV-----DSGTHSIEIFTLLPSRLYWVTVAAVNGAGVG 555

  Fly  1102 PLSEPTAAQTMEDVPSRPPEDVRCAALSSQSLQVSWQPPPIY-HTNGLLQGYKLIFEPII---DD 1162
            ..|:|........:.:.|.:    ..:.|.|..||....|:: .:.|.|....|:...:.   ..
Zfish   556 VQSDPYKLLIESRLEATPYQ----TGILSMSHFVSVIKEPVFIGSVGTLLWCVLMITAVFLYRRH 616

  Fly  1163 IQPSKDEVESRKTTALTMVLTGLRKYTNYSIQVLAHTRMGDGVVSKPLFCHSEEDVPEAPADIKV 1227
            |:|:....:|          :||.:..:..: ::.| ||.               .|::|    .
Zfish   617 IRPANPSGKS----------SGLYRLESEDL-IIKH-RMA---------------APDSP----W 650

  Fly  1228 VS------SSSQSLYISWLPPNEPNGVITKYSLYTRVVNGREELNNEKRSLPSQQAYYEAKGLHP 1286
            :|      |:|:.....|.|..|.:| :.:.:|...|......|.:....:|.....|..
Zfish   651 ISGGWRPGSNSEPKQGLWTPSQENSG-LRRTTLPITVKKDPNPLRSAVPIVPDNCGVYGT----- 709

  Fly  1287 HMEYQFWVTAS-------------TRVGEGKSSRVSSQITTNRIPARIISFGGPVVR-----PWR 1333
                 |:|..:             .|:..|.|...:|:  |.||...|:...  |||     ||:
Zfish   710 -----FYVDLTGNGLKTFNSPGRCPRMPHGNSQLQNSE--TIRITEPIVKTA--VVRELQALPWK 765

  Fly  1334 STVTLPCTA-VGKPKREWFKSD------------VALRQ--------------------GG---- 1361
            .  |||... :|..|..|.|:.            :.|.|                    ||
Zfish   766 R--TLPAQPNMGVKKESWEKNVKRELHAVKSAPLMPLNQQALAVCSAPNHQQRFSQHPAGGVSEP 828

  Fly  1362 ---------LHNSQLLDSGDLIIS--SLQLADGGNYSCQVDNGIGTDRLTHTLIVQVPPTAPVLY 1415
                     ||.|..|...|::.|  .|.:.|..:.|.: |....:.:||
Zfish   829 VKPLSSPRILHYSASLQLVDMLPSPPPLPMEDNHSLSSE-DESTRSTKLT--------------- 877

  Fly  1416 VTSATSSSILMHWKCGFTG-NAPITGYTLFYRRANGNTDEMQLSRHASSHELKGLMCGST--YQ- 1476
            |...:..|:     |..:| ..|.:..|    ....|....||| .:.||......|.||  ||
Zfish   878 VDIGSQQSV-----CAASGLQGPASANT----GCPNNHSPAQLS-PSYSHLSTASFCLSTDDYQD 932

  Fly  1477 IHLSAQNK---VGTSPTSTILHVRTQGQSPGHPASTALLAPNS 1516
            ..||.|..   :..||       :.||||..|.::.::..|.|
Zfish   933 TTLSTQGHTQYMEISP-------KPQGQSTSHQSTPSMPRPFS 968

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Dscam2	NP_001261500.1	Ig	51..127	CDD:299845
		Ig	138..>203	CDD:299845
		I-set	238..327	CDD:254352
		Ig	247..327	CDD:299845
		I-set	332..418	CDD:254352
		IGc2	344..407	CDD:197706
		IG_like	432..517	CDD:214653
		IGc2	436..507	CDD:197706
		I-set	521..610	CDD:254352	9/49 (18%)
		IGc2	533..597	CDD:197706	6/36 (17%)
		Ig	630..699	CDD:143165	19/82 (23%)
		IG_like	714..802	CDD:214653	22/91 (24%)
		Ig	725..802	CDD:299845	19/80 (24%)
		Ig	823..894	CDD:143165	21/76 (28%)
		FN3	906..1006	CDD:238020	25/103 (24%)
		FN3	1013..1111	CDD:238020	28/99 (28%)
		FN3	1119..1209	CDD:238020	17/93 (18%)
		FN3	1219..1313	CDD:238020	20/112 (18%)
		Ig	1336..1402	CDD:143165	23/113 (20%)
		FN3	1409..1498	CDD:238020	22/95 (23%)
		FN3	1515..1588	CDD:238020	1/2 (50%)
robo4	XP_689255.3	Ig1_Robo	70..169	CDD:143317	22/106 (21%)
		I-set	71..168	CDD:254352	22/104 (21%)
		I-set	175..261	CDD:254352	23/95 (24%)
		Ig2_Robo	177..261	CDD:143201	23/93 (25%)
		I-set	265..350	CDD:254352	27/101 (27%)
		Ig	282..350	CDD:299845	23/84 (27%)
		FN3	373..448	CDD:214495	18/79 (23%)
		FN3	472..560	CDD:238020	27/93 (29%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3510
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR10075
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	4	3.910