DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Best2 and Best1

DIOPT Version :9

Sequence 1:	NP_729159.2	Gene:	Best2 / 38727	FlyBaseID:	FBgn0035696	Length:	809	Species:	Drosophila melanogaster
Sequence 2:	NP_001247018.1	Gene:	Best1 / 53431	FlyBaseID:	FBgn0040238	Length:	784	Species:	Drosophila melanogaster

Alignment Length:	829	Identity:	286/829 - (34%)
Similarity:	416/829 - (50%)	Gaps:	175/829 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MTVSYAGEVPNGSSFGCFWKILWKWRGSVYKLIWRELVAYLCLYYTINVIYRFGLTESQQAIFKK 65
            ||::|.|||.....||||.|:|.:||||:|||:|.:|:|:|.:||.||::|||||..:|:..|:.
  Fly     1 MTITYTGEVATCRGFGCFLKLLLRWRGSIYKLVWLDLLAFLTIYYAINMVYRFGLNPAQKETFEA 65

  Fly    66 IRLYFGQQSESIPMSFVLGFYVNLVVKRWWEQYRLLPWPDTLALFISAAIPNSNGGVNNETGRLM 130
            |..|.....|.||:||||||||::|:.|||.||..:||||.:|:|:|:   |.:|  .:|.||:|
  Fly    66 IVQYCDSYRELIPLSFVLGFYVSIVMTRWWNQYTSIPWPDPIAVFVSS---NVHG--QDERGRMM 125

  Fly   131 RRNIMRYMVLAYVITLQRISLRVKRRFPTTQHLVDAGLMHESEMKIFEALNQKSPM-SKYWMPLV 194
            ||.||||:.|...:.|..:|.|||:|||...:||:|||::::|..|.|.:|:..|. ||:|:|:|
  Fly   126 RRTIMRYVCLCLTMVLANVSPRVKKRFPGLNNLVEAGLLNDNEKTIIETMNKAFPRPSKHWLPIV 190

  Fly   195 WATNIINRARKDGLIASDHIVQTILVELSDIRRRLGGLIGYDTVCVPLVYTQVVTLVLYTYFIAA 259
            ||.:||.||||:|.|..|..|:||:.||:..|.:.|.||.|||:.|||||||||||.:|:||:..
  Fly   191 WAASIITRARKEGRIRDDFAVKTIIDELNKFRGQCGLLISYDTISVPLVYTQVVTLAVYSYFLTC 255

  Fly   260 LLGRQMLPN--VLDRSGREDPDLFFPLFTVLQFVFYVGWLKVAEVLINPFGEDDDDIELNWLIDR 322
            .:|:|....  |.:.:.....||:||:||.|||.||:|||||||.|||||||||||.|:||::||
  Fly   256 CMGQQWTDGKVVGNTTYLNKVDLYFPVFTTLQFFFYMGWLKVAESLINPFGEDDDDFEVNWMVDR 320

  Fly   323 HIKAAYMIVDEMHEEHPELLRDQYWECVVPKDLPYTVASEHYRKDEPKGSAEKYKVKKEDAMYAN 387
            :::.:|:||||||.:|||||:||||:.|.|.:||||:|:|.:|::.|:.|..|.:|.|..||.:.
  Fly   321 NLQVSYLIVDEMHHDHPELLKDQYWDEVFPNELPYTIAAERFRENHPEPSTAKIEVPKNAAMPST 385

  Fly   388 I---------------------------------MPGG--GKRMLSDDV---YADYESVDTPMVE 414
            :                                 :||.  |.:.::.|.   ..|.|:.|...:.
  Fly   386 MSSVRIDEMVGNANGDPAAAADAKAPNPVTGQVQVPGADTGVQTVTYDFPKGSPDEEADDASGIH 450

  Fly   415 RRKNNWLVRQLSRMGSMR---SQSTAYSSGGMPFNRNRLNSVYSS-----------PESGLPLTI 465
            ....|         |.||   |.|....||.:    :|:|:|.|:           |.|..|
  Fly   451 FSAGN---------GKMRLDSSPSLVSVSGTL----SRVNTVASALKRFLSRDDSRPGSATP--- 499

  Fly   466 LQQQQLQQAHQQQQAGSQPSKSSLYGKFVHRK--------SLRAQRQLIKQNSKLNGLNVNVAKT 522
                   ...|..:..:..|.:||.|..|...        |||..:|:|::..:    ...:...
  Fly   500 -------SQDQPYKFPASASSASLSGAVVGSATSAGKPAGSLRITQQVIEEVDE----QATITSM 553

  Fly   523 RPRIPTP---EVAKDGNTNPATSSVLMAP----QQLSTTSAPP--GMYPSSYAPDTLLHQESGQV 578
            |...|.|   ::....::...||..|..|    :.:...|.||  ....|.|.|:
  Fly   554 RANDPRPNVMDIFAQTSSGAGTSGPLQPPPAHSEPVDIPSRPPSYNRAQSQYEPN---------- 608

  Fly   579 LGTLLLSPIKEMDSSSSNNTLIPGHP---ATAALAASMKEGFTPSSI-------------PAATN 627
                 |.|...:|:..|.:....|.|   :.||.|.|...|.:..|:             |.|..
  Fly   609 -----LFPPGGVDALLSTSAPAGGSPLLLSNAATAPSSPVGESSKSLYDPQKGASRETERPDAPQ 668

  Fly   628 ITTLSFPFSVTSTGGETMP-------TGTLSILP-------------------ISANAQLPTITT 666
            :    |.:.||..  |:|.       .|..::.|                   :.....|....:
  Fly   669 V----FRWFVTPV--ESMDLRSSTDLLGDAAVQPEDEGDDFDKLKAEREKEKLMRQQKNLARTIS 727

  Fly   667 TASGYDPNDV--ITTIPVSLSIQRS---PSALSIVELTGADVQDGYSNS 710
            ||.|.:...|  :..:||::::|::   |.|.|...|.|.   |.:|||
  Fly   728 TAPGMEATAVPMVPMVPVNVAVQQAQLQPVASSADLLAGG---DQFSNS 773

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Best2	NP_729159.2	Bestrophin	13..324	CDD:279411	164/313 (52%)
Best1	NP_001247018.1	Bestrophin	8..322	CDD:279411	166/318 (52%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	282	1.000	Domainoid score	I871
eggNOG	1	0.900	-	-		E1_KOG3547
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D145964at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm6580
orthoMCL	1	0.900	-	-		OOG6_101870
Panther	1	1.100	-	-	P	PTHR10736
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X320
	8	7.820