DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Mdr65 and STE6

DIOPT Version :9

Sequence 1:	NP_476831.1	Gene:	Mdr65 / 38726	FlyBaseID:	FBgn0004513	Length:	1302	Species:	Drosophila melanogaster
Sequence 2:	NP_012713.1	Gene:	STE6 / 853671	SGDID:	S000001692	Length:	1290	Species:	Saccharomyces cerevisiae

Alignment Length:	1307	Identity:	335/1307 - (25%)
Similarity:	588/1307 - (44%)	Gaps:	145/1307 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly    62 LPAVVIIYSEFTSMLVDRAMQFGTSSNVHALPLFGGGKTLTNASREENNEALYDDSI--SYGIL- 123
            :||:       ||:|..|....        |.:|     :.|.|    ::.||...:  |..::
Yeast    40 VPAI-------TSILTGRVFDL--------LSVF-----VANGS----HQGLYSQLVQRSMAVMA 80

  Fly   124 LTIASV-VMFISGIFSVDVFNMVALRQVTRMRIKLFSSVIRQ-DIGWHDLASK--QNFTQSMVDD 184
            |..||| ||::    |:..:..:..||..|:|.::..:.:.: .:.|:|...|  .:||| :...
Yeast    81 LGAASVPVMWL----SLTSWMHIGERQGFRIRSQILEAYLEEKPMEWYDNNEKLLGDFTQ-INRC 140

  Fly   185 VEKIRDGISEKVGHFVYLVVGFIITVAISFSYGWKLTLAVSSYIPLVILLNYYVAKFQGKLTARE 249
            ||::|...:|........:|.....:..||.|.|.|||.:....|::.......::.....:.:|
Yeast   141 VEELRSSSAEASAITFQNLVAICALLGTSFYYSWSLTLIILCSSPIITFFAVVFSRMIHVYSEKE 205

  Fly   250 QESYAGAGNLAEEILSSIRTVVSFGGEKSEVQRYENFLVPARKASQWKGAFSGLSDAVLKSMLYL 314
            ....:.|..|....:::.:.|..:..::.|.::::..::...........|...:..:|:.:...
Yeast   206 NSETSKAAQLLTWSMNAAQLVRLYCTQRLERKKFKEIILNCNTFFIKSCFFVAANAGILRFLTLT 270

  Fly   315 SCAGAFWYGVNLIIDDR-NVENKEYTPAILMIAFFGIIVGADNIARTAPFLESFATARGCATNLF 378
            .....||:|..:|...: |:.:       ::..|...|:....:..|   |......:.....:.
Yeast   271 MFVQGFWFGSAMIKKGKLNIND-------VITCFHSCIMLGSTLNNT---LHQIVVLQKGGVAME 325

  Fly   379 KVIDLT---SKIDPLSTDGKL------LNYGLRGDVEFQDVFFRYPSRPEVIVHRGLNIRIRAGQ 434
            |::.|.   ||.:||:   |.      |:|. ..|:.|.:|.|.|||||...|.:.:::...|||
Yeast   326 KIMTLLKDGSKRNPLN---KTVAHQFPLDYA-TSDLTFANVSFSYPSRPSEAVLKNVSLNFSAGQ 386

  Fly   435 TVALVGSSGCGKSTCVQLLQRFYDPVFGSVLLDDLDIRKYNIQWLRSNIAVVGQEPVLFLGTIAQ 499
            ...:||.||.||||...||.||||...||:.::..:|:..:.:.|..||.||.|...||..|:.:
Yeast   387 FTFIVGKSGSGKSTLSNLLLRFYDGYNGSISINGHNIQTIDQKLLIENITVVEQRCTLFNDTLRK 451

  Fly   500 NISYGKPGATQKE----------IEAAATQAGAHEFITNLPESYRSMIGERGSQLSGGQKQRIAI 554
            ||..|...:.:..          |:.|...|....||.:||:...::||..|..|||||:||:||
Yeast   452 NILLGSTDSVRNADCSTNENRHLIKDACQMALLDRFILDLPDGLETLIGTGGVTLSGGQQQRVAI 516

  Fly   555 ARALIQNPKILLLDEATSALDYQSEKQVQQALDLASKGRTTIVVSHRLSAIRGADKIVFIHDGKV 619
            |||.|::..||.||||.||||......:.:|:....||:|||:::|.||.|...|.:..:.:|:|
Yeast   517 ARAFIRDTPILFLDEAVSALDIVHRNLLMKAIRHWRKGKTTIILTHELSQIESDDYLYLMKEGEV 581

  Fly   620 LEEGSHDDLMA-----------LEGAYYNMVRAGDINMPDE----VEKEDSIEDTKQKSLALFEK 669
            :|.|:..:|:|           |:..|.:.....|....::    ||..:|..:|.:....|...
Yeast   582 VESGTQSELLADPTTTFSTWYHLQNDYSDAKTIVDTETEEKSIHTVESFNSQLETPKLGSCLSNL 646

  Fly   670 SF-ETSPLNFEKG--QKNS------VQFEEPIIKALIKDTNAQSAEAPPEKPNFFRTFSRILQLA 725
            .: ||..|:|.:.  ||.|      |:.||..|...:|......:...|:..:..:...|:::..
Yeast   647 GYDETDQLSFYEAIYQKRSNVRTRRVKVEEENIGYALKQQKNTESSTGPQLLSIIQIIKRMIKSI 711

  Fly   726 KQEWCYLILGTISAVAVGFLYPAFAVIFGEFYAALAEKDPEDALRRTAVLSWA--CLGLAFLTGL 788
            :.: ..||||.:.::..|...|.|:..|......:.............:..|:  .||:|...|:
Yeast   712 RYK-KILILGLLCSLIAGATNPVFSYTFSFLLEGIVPSTDGKTGSSHYLAKWSLLVLGVAAADGI 775

  Fly   789 VCFLQTYLFNYAGIWLTTRMRAMTFNAMVNQEVGWFDDENNSVGALSARLSGEAVDIQGAIGYPL 853
            ..|.:.:|.:....:....:|......:..:.:.||..|||....:||.:..:..|::..:
Yeast   776 FNFAKGFLLDCCSEYWVMDLRNEVMEKLTRKNMDWFSGENNKASEISALVLNDLRDLRSLV---- 836

  Fly   854 SGMIQALSNFISSVSV----AMYYNWKLALLCLANCPIIVGSVILEAKMMSNAVVREKQVIEEAC 914
            |..:.|:::|::..::    |:...|||:|:|::..|:|:....:...::.......|..:.:..
Yeast   837 SEFLSAMTSFVTVSTIGLIWALVSGWKLSLVCISMFPLIIIFSAIYGGILQKCETDYKTSVAQLE 901

  Fly   915 RIATESITNIRTVAGLRREADVIREYTEEIQRVEVLIRQKLRWRGVLNSTMQASAF---FAYAVA 976
            ....:.:|||:|:..|:.|......|.:          .|::.:.:.:....|:.|   ....:.
Yeast   902 NCLYQIVTNIKTIKCLQAEFHFQLTYHD----------LKIKMQQIASKRAIATGFGISMTNMIV 956

  Fly   977 LC-------YGGVLVSEGQLPFQDIIKVSETLLYGSMMLAQSLAFTPAFSAALIAGHRLFQILDR 1034
            :|       ||..||...:...:::......||:..|.....::..|..|....|...:::|||.
Yeast   957 MCIQAIIYYYGLKLVMIHEYTSKEMFTTFTLLLFTIMSCTSLVSQIPDISRGQRAASWIYRILDE 1021

  Fly  1035 KPKIQSPMGTIKNTLAKQLNLFEGVRYRG-----------IQFRYPTRPDAKILNGLDLEVLKGQ 1088
            |.....    ::|..|:.:.: .|..|.|           :.|.||:.|.|.:...::.::..||
Yeast  1022 KHNTLE----VENNNARTVGI-AGHTYHGKEKKPIVSIQNLTFAYPSAPTAFVYKNMNFDMFCGQ 1081

  Fly  1089 TVALVGHSGCGKSTCVQLLQRYYDPDEGTIHIDHDDIQHDLTLDGVRTKLGIVSQEPTLFERSIA 1153
            |:.::|.||.||||.|.||.:.|:.:.|.|.||..|: :|..|..:|.::.:|.|:|.||..:|.
Yeast  1082 TLGIIGESGTGKSTLVLLLTKLYNCEVGKIKIDGTDV-NDWNLTSLRKEISVVEQKPLLFNGTIR 1145

  Fly  1154 ENIAYGDNRRSVSMVEIIAAAKSANAHSFIISLPNGYDTRMGARGTQLSGGQKQRIAIARALVRN 1218
            :|:.|| .:..:..:|:..|.|....|.|:||.|.|.|||:..  |.|||||.||:.|||||:|.
Yeast  1146 DNLTYG-LQDEILEIEMYDALKYVGIHDFVISSPQGLDTRIDT--TLLSGGQAQRLCIARALLRK 1207

  Fly  1219 PKILLLDEATSALDLQSEQLVQQALDTACSGRTCIVIAHRLSTVQNADVICVIQNGQVVEQGNHM 1283
            .|||:|||.|||||..|..::.:.:.........:||.|....:::.:.|.|:::|:|||:||..
Yeast  1208 SKILILDECTSALDSVSSSIINEIVKKGPPALLTMVITHSEQMMRSCNSIAVLKDGKVVERGNFD 1272

  Fly  1284 QLISQGG 1290
            .|.:..|
Yeast  1273 TLYNNRG 1279

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Mdr65	NP_476831.1	PTZ00265	91..1300	CDD:240339	329/1278 (26%)
		ABC_membrane	108..354	CDD:279056	47/253 (19%)
		ABC_MTABC3_MDL1_MDL2	405..642	CDD:213216	93/257 (36%)
		ABC_membrane	736..1006	CDD:279056	49/285 (17%)
		ABC_MTABC3_MDL1_MDL2	1059..1299	CDD:213216	89/243 (37%)
STE6	NP_012713.1	MdlB	24..605	CDD:224055	160/607 (26%)
STE6	NP_012713.1	MdlB	702..1289	CDD:224055	154/602 (26%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	76	1.000	Domainoid score	I2107
eggNOG	1	0.900	-	-		E2759_KOG0055
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	448	1.000	Inparanoid score	I251
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000106
OrthoInspector	1	1.000	-	-		otm46468
orthoMCL	1	0.900	-	-		OOG6_100059
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X92
TreeFam	1	0.960	-	-
	8	7.810