Protein Alignment Klp64D and KIF14

DIOPT Version :10

• Sequence 1: NP_523934.1
• Gene: Klp64D / 38611
• FlyBaseID: FBgn0004380
• Length: 677
• Species: Drosophila melanogaster
• Sequence 2: NP_055690.1
• Gene: KIF14 / 9928
• HGNCID: 19181
• Length: 1648
• Species: Homo sapiens
• Alignment Length: 761
• Identity: 217/761 - (28%)
• Similarity: 339/761 - (44%)
• Gaps: 175/761 - (22%)

  Fly     1 MPQEEANAGTTAQLDD--EIEN--VRVVVRTRPMDKNELSAGALSAISVDKINRAITVMKPNATA 61
            :|:||.....|:...|  ::||  |.|.||.||..|.|         .::|.::.:.:.....|.
Human   335 LPEEETVVQNTSAGKDPLKVENSQVTVAVRVRPFTKRE---------KIEKASQVVFMSGKEITV 390

  Fly    62 NEPPKTYYFDNVFDGG-----------SNQMDLYVDTARPIVDKVLEGYNGTILAYGQTGTGKTY 115
            ..|.....::.::|..           ::|..:|...|.|::::..||:|..:.||||||:||:|
Human   391 EHPDTKQVYNFIYDVSFWSFDECHPHYASQTTVYEKLAAPLLERAFEGFNTCLFAYGQTGSGKSY 455

  Fly   116 TMSGNPDSPQTKGIIPNAFAHIFGHIAKAKENQK--FLVRVSYMEIYNEEVRDLL-GKDVG---- 173
            ||.|..:.|   ||||.....:|..:|: |:.|:  :.:.:|:.|:|||::.||| .||..
Human   456 TMMGFSEEP---GIIPRFCEDLFSQVAR-KQTQEVSYHIEMSFFEVYNEKIHDLLVCKDENGQRK 516

  Fly   174 KSLEVKERPDIGVFVKDLSGYVVHNADDLENIMRLGNKNRAVGATKMNQESSRSHAIFSI--TVE 236
            :.|.|:|.|..|.:|:.||..:|.:..|:::.:.||||.||..||.||.:|||||::|::  |..
Human   517 QPLRVREHPVYGPYVEALSMNIVSSYADIQSWLELGNKQRATAATGMNDKSSRSHSVFTLVMTQT 581

  Fly   237 RSELGEGDVQHVRM-GKLQLVDLAGSERQSKTQASGQRLKEATKINLSLSVLGNVISAL---VDG 297
            ::|..||:....|: .::.|:|||||||.|....:|.||||...||.||..||.|||||   .:.
Human   582 KTEFVEGEEHDHRITSRINLIDLAGSERCSTAHTNGDRLKEGVSINKSLLTLGKVISALSEQANQ 646

  Fly   298 KSTHIPYRNSKLTRLLQDSLGGNSKTVMCATISPADSNYMETISTLRYASRAKNIQNRMHINEEP 362
            :|..||||.|.||.||::||||||||.|.||||||.||..||:||||||::|:.|.|...:||:.
Human   647 RSVFIPYRESVLTWLLKESLGGNSKTAMIATISPAASNIEETLSTLRYANQARLIVNIAKVNEDM 711

  Fly   363 KDALLRHFQEEIARL------------------RKQL---------EEGDSLEEEPPSSEEEEDT 400
            ...|:|..:.|||:|                  |:::         :|.|..|.:....|:.|..
Human   712 NAKLIRELKAEIAKLKAAQRNSRNIDPERYRLCRQEITSLRMKLHQQERDMAEMQRVWKEKFEQA 776

  Fly   401 ADDELEAPLEIELESSTIQAVEKKPKKKREKTDAEKEELA--------------KRKNEHQKEIE 451
            ...:|:...|::......|.....|.......|.:..|:.              |..:.|..::.
Human   777 EKRKLQETKELQKAGIMFQMDNHLPNLVNLNEDPQLSEMLLYMIKEGTTTVGKYKPNSSHDIQLS 841

  Fly   452 HAKTEQE--TLRN--KLVSL----EGKILVGGENLL----------------------------- 479
            ......:  |::|  ..||:    |.|..|.|:::|
Human   842 GVLIADDHCTIKNFGGTVSIIPVGEAKTYVNGKHILEITVLRHGDRVILGGDHYFRFNHPVEVQK 906

  Fly   480 ------------------EKAQTQELLLEQSIAELEQHE---KSEEALKQTLQ------------ 511
                              |.|:.:.|:.::|..|.|..|   |::|.:.|.:|
Human   907 GKRPSGRDTPISEGPKDFEFAKNELLMAQRSQLEAEIKEAQLKAKEEMMQGIQIAKEMAQQELSS 971

  Fly   512 QKATERIDIEERYSTLQDASTGITKKIHRVMQMLMGVKSELADQQQEHQREKEGIYENIRSLSRE 576
            |||.....|:...:.|::.|.  .||:..:.......|.|..::.::|..::  ||.|.:.|..|
Human   972 QKAAYESKIKALEAELREESQ--RKKMQEINNQKANHKIEELEKAKQHLEQE--IYVNKKRLEME 1032

  Fly   577 LALCELVLNSY-----------------VPKEYQSMINQYTHWNED 605
            ....:..|..:                 :.||.|  |.|....|.|
Human  1033 TLATKQALEDHSIRHARILEALETEKQKIAKEVQ--ILQQNRNNRD 1076

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp64D	NP_523934.1	KISc_KIF3	19..352	CDD:276822	144/358 (40%)
		Smc	<433..>578	CDD:440809	40/228 (18%)
KIF14	NP_055690.1	Required for PRC1-binding	1..356		5/20 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..27
		Required for microtubule-binding with high affinity. /evidence=ECO:0000250|UniProtKB:L0N7N1	356..737		153/393 (39%)
		KISc_KIF1A_KIF1B	357..708	CDD:276816	144/363 (40%)
		Kinesin_assoc	705..823	CDD:465047	20/117 (17%)
		FHA_KIF14	794..901	CDD:438759	15/106 (14%)
		Required for CIT-binding	901..1648		35/182 (19%)
		Smc	<927..>1077	CDD:440809	34/156 (22%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1600..1648