Protein Alignment Klp64D and Kif5a

DIOPT Version :10

• Sequence 1: NP_523934.1
• Gene: Klp64D / 38611
• FlyBaseID: FBgn0004380
• Length: 677
• Species: Drosophila melanogaster
• Sequence 2: NP_997688.1
• Gene: Kif5a / 314906
• RGDID: 1303035
• Length: 1027
• Species: Rattus norvegicus
• Alignment Length: 736
• Identity: 226/736 - (30%)
• Similarity: 345/736 - (46%)
• Gaps: 146/736 - (19%)

  Fly    12 AQLDDEIENVRVVVRTRPMDKNELSAGALSAISVDKINRAITVMKPNATANEPPKTYYFDNVFDG 76
            |:.::|. :::|:.|.||:::.|:..|       ||.   |.:.:.:.:.....|.|.||.||..
  Rat     2 AETNNEC-SIKVLCRFRPLNQAEILRG-------DKF---IPIFQGDDSVIIGGKPYVFDRVFPP 55

  Fly    77 GSNQMDLYVDTARPIVDKVLEGYNGTILAYGQTGTGKTYTMSGNPDSPQTKGIIPNAFAHIFGHI 141
            .:.|..:|...|..||..||.||||||.|||||.:|||:||.|....||..||||.....||.||
  Rat    56 NTTQEQVYHACAMQIVKDVLAGYNGTIFAYGQTSSGKTHTMEGKLHDPQLMGIIPRIARDIFNHI 120

  Fly   142 AKAKENQKFLVRVSYMEIYNEEVRDLLGKDVGK-SLEVKERPDIGVFVKDLSGYVVHNADDLENI 205
            ....||.:|.::|||.|||.:::||||  ||.| :|.|.|..:...||:..:...|.:.:::.::
  Rat   121 YSMDENLEFHIKVSYFEIYLDKIRDLL--DVTKTNLSVHEDKNRVPFVRGCTERFVSSPEEILDV 183

  Fly   206 MRLGNKNRAVGATKMNQESSRSHAIFSITVERSELGEGDVQHVRMGKLQLVDLAGSERQSKTQAS 270
            :..|..||.|..|.||:.|||||:||.|.:::..:   :.:....|||.|.||||||:.|||.|.
  Rat   184 IDEGKSNRHVAVTNMNEHSSRSHSIFLINIKQENI---ETEQKLSGKLYLADLAGSEKVSKTGAE 245

  Fly   271 GQRLKEATKINLSLSVLGNVISALVDGKSTHIPYRNSKLTRLLQDSLGGNSKTVMCATISPADSN 335
            |..|.||..||.|||.||||||||.:|..:::|||:||:||:||||||||.:|.|....||:..|
  Rat   246 GAVLDEAKNINKSLSALGNVISALAEGTKSYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYN 310

  Fly   336 YMETISTLRYASRAKNIQNRMHIN------------EEPKDALLRHFQEEIARLRKQL---EEGD 385
            ..||.|||.:..|||.|:|...:|            |:.|:. .:..:|.||:|..:|   ..|:
  Rat   311 DAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEKEK-TKAQKETIAKLEAELSRWRNGE 374

  Fly   386 SL-EEEPPSSEEEEDTADDELEAPLEIELESSTIQAVEKKPKKKREKTDAEKEELAKRKNEHQKE 449
            :: |.|..:.|:....|:...|.|:.   ::|:|  |.:...::|:|.:.|...|.|:.::...|
  Rat   375 NVPETERLAGEDSALAAEICEETPVN---DNSSI--VVRIAPEERQKYEEEIRRLYKQLDDKDDE 434

  Fly   450 IEHAKTEQETLRNKLVSLEGKILVG----------------GENLLEKAQTQELL---------L 489
            |.......|.|:.:::..| ::||.                .||...|.:.:|:|         .
  Rat   435 INQQSQLIEKLKQQMLDQE-ELLVSTRGDNEKVQRELSHLQSENDAAKEEVKEVLQALEELAVNY 498

  Fly   490 EQSIAELEQHEKSEEALKQTLQQKATERIDIEERYSTLQDASTGITKKIHRVMQMLM-------- 546
            :|...|:|:..:..:.|...|.||....:.:|.....||:.|....|:|..|:..||
  Rat   499 DQKSQEVEEKSQQNQLLVDELSQKVATMLSLESEPQRLQEVSGHQRKRIAEVLNGLMKDLSEFSV 563

  Fly   547 -----------------------------GVKSE----------LADQQQEHQREKEGIYENIRS 572
                                         .:|||          |.:.|.|..|:.|       .
  Rat   564 IVGNGEIKLPVEISGAIEEEFTVARLYISKIKSEVKSVVKRCRQLENLQVECHRKME-------V 621

  Fly   573 LSRELALCELVLNSYVPK-----EYQSMI--------NQYTHWNEDIGEWQLKCVAYTGNNMRKH 624
            ..|||:.|:|:::.:..|     ||...:        ..|...::::.:.|.:
  Rat   622 TGRELSSCQLLISQHEAKIRSLTEYMQTVELKKRHLEESYDSLSDELAKLQAQ------------ 674

  Fly   625 ISAHKTS--GKEPDFLDLSHV 643
            .:.|:.:  .||||..|...|
  Rat   675 ETVHEVALKDKEPDTQDAEEV 695

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp64D	NP_523934.1	KISc_KIF3	19..352	CDD:276822	146/333 (44%)
		Smc	<433..>578	CDD:440809	41/216 (19%)
Kif5a	NP_997688.1	KISc_KHC_KIF5	7..327	CDD:276820	147/335 (44%)
		Microtubule-binding. /evidence=ECO:0000255	174..315		69/143 (48%)
		Necessary for interaction with ZFYVE27. /evidence=ECO:0000250|UniProtKB:P33175	271..361		35/90 (39%)
		Interaction with BICD2. /evidence=ECO:0000250|UniProtKB:Q12840	353..1027		73/368 (20%)
		PRK03918	<409..920	CDD:235175	58/307 (19%)
		Khc_CBD_cc	832..901	CDD:467880
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	906..936
		Globular. /evidence=ECO:0000255	907..1027