DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Gen and Ercc5

DIOPT Version :9

Sequence 1:	NP_647943.2	Gene:	Gen / 38594	FlyBaseID:	FBgn0263831	Length:	726	Species:	Drosophila melanogaster
Sequence 2:	NP_035859.2	Gene:	Ercc5 / 22592	MGIID:	103582	Length:	1170	Species:	Mus musculus

Alignment Length:	534	Identity:	116/534 - (21%)
Similarity:	195/534 - (36%)	Gaps:	166/534 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly    64 WEQVTPVFVLEGVAPKLKSQVIAKRNELQFRGVKPKNSPECTQSQPSKGDK-GRSRFNHVLKQCE 127
            |:.:.    ||.: ..|:|.::|::|.|              ::|..:.|: ..|....:..:.:
Mouse   727 WQDIN----LEEL-DALESNLLAEQNSL--------------KAQKQQQDRIAASVTGQMFLESQ 772

  Fly   128 TLLLSMGIQCVQGPGEAEAYCAFLNKHGLVDGVISQDSDCFAYGAVRVYRNFSVSTQGAQAAAGG 192
            .||...|:..:|.|.||||.||.|:......|.|:.|||.:.:||..||:||....:
Mouse   773 ELLRLFGVPYIQAPMEAEAQCAMLDLTDQTSGTITDDSDIWLFGARHVYKNFFNKNK-------- 829

  Fly   193 AVDIYDMREITSRMDFGQQKIIVMALLCGCDYCPDGIGGIGKDGVLKLFNKY--KETEILDRMRS 255
            .|:.|...:..|::...:.|:|.:|.|.|.|| .:||..:|....:::.|::  :..:.|.:...
Mouse   830 FVEYYQYVDFYSQLGLDRNKLINLAYLLGSDY-TEGIPTVGCVTAMEILNEFPGRGLDPLLKFSE 893

  Fly   256 WRGETDKYNALEIRVDDKSICSNCGHIGKTQSHTKSGCSVCRTHKGCDESLWKEQRLSIKSELTL 320
            |..|..         ::|.:..|       ...||                       :|.:|
Mouse   894 WWHEAQ---------NNKKVAEN-------PYDTK-----------------------VKKKL-- 917

  Fly   321 RRKALLSPDFPNEEIIAEFLSEPDTIPNLNLNWRQPNLVKFIKQIGHLLQWPEIYCFQKFFPIL- 384
             ||..|:|.|||..:...:|.........:..|.:|::.|..:.......|..:...:..:|:|
Mouse   918 -RKLQLTPGFPNPAVADAYLRPVVDDSRGSFLWGKPDVDKIREFCQRYFGWNRMKTDESLYPVLK 981

  Fly   385 ------TRWQV-------QQSKQEKILIQPHEIIKKRTVKGVPSLELRWHDPSGIFKGLIPDKQI 436
                  |:.::       ||.||:..||:.|.:  .|.|..:    ||
Mouse   982 HLNAHQTQLRIDSFFRLAQQEKQDAKLIKSHRL--NRAVTCI----LR----------------- 1023

  Fly   437 AEYEAEHPKGIEELYYTIEPLDMLETAYPDLVAAFLKSKEKPAKKTTRKKKTASEEENKENEPNS 501
            .|.|.:.|    ||....|.||                   .||..|:|::...::|      .|
Mouse  1024 KEREEKAP----ELTKVTEALD-------------------DAKGKTQKRELPYKKE------TS 1059

  Fly   502 KPKRVVRKIKAQPEENQPLLHQFLGRKKEGTPVKAPAPQRQQC-----STPITKFLPSDLES--- 558
            .|||      .:|..|...|         |.|..:.:||...|     |:.::....|..||
Mouse  1060 VPKR------RRPSGNGGFL---------GDPYCSESPQESSCEDGEGSSVMSARQRSAAESSKI 1109

  Fly   559 DCDAEEFDMSDIVK 572
            .|.    |:.|:|:
Mouse  1110 SCS----DVPDLVR 1119

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Gen	NP_647943.2	PIN_GEN1	1..385	CDD:189039	71/330 (22%)
Gen	NP_647943.2	H3TH_GEN1	210..342	CDD:188625	28/133 (21%)
Ercc5	NP_035859.2	N-domain. /evidence=ECO:0000250\|UniProtKB:P28715	1..78
		DNA-binding, may bind to the undamaged single-strand DNA of the DNA repair bubble. /evidence=ECO:0000250\|UniProtKB:P28715	31..67
		Spacer region. /evidence=ECO:0000250\|UniProtKB:P28715	79..784		14/75 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	304..479
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	520..587
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	600..701
		I-domain. /evidence=ECO:0000250\|UniProtKB:P28715	785..880		33/103 (32%)
		DNA-binding, may bind to the undamaged single-strand DNA of the DNA repair bubble. /evidence=ECO:0000250\|UniProtKB:P28715	819..835		5/23 (22%)
		DNA-binding, H2TH (helix-2turn-helix) motif which binds double-stranded DNA. /evidence=ECO:0000250\|UniProtKB:P28715	847..879		10/32 (31%)
		DNA-binding, may bind double-stranded DNA. /evidence=ECO:0000250\|UniProtKB:P28715	911..917		3/28 (11%)
		Interaction with PCNA. /evidence=ECO:0000250\|UniProtKB:P28715	980..1008		6/27 (22%)
		Interaction with ERCC6/CSB. /evidence=ECO:0000250\|UniProtKB:P28715	1010..1170		38/181 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1033..1146		29/131 (22%)
		Nuclear localization signal 1. /evidence=ECO:0000250\|UniProtKB:P28715	1049..1065		6/27 (22%)
		Nuclear localization signal 2. /evidence=ECO:0000250\|UniProtKB:P28715	1153..1170
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0258
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810