Protein Alignment RhoGEF64C and TUS1

DIOPT Version :10

• Sequence 1: NP_729032.1
• Gene: RhoGEF64C / 38578
• FlyBaseID: FBgn0035574
• Length: 1984
• Species: Drosophila melanogaster
• Sequence 2: NP_013529.3
• Gene: TUS1 / 851145
• SGDID: S000004417
• Length: 1307
• Species: Saccharomyces cerevisiae
• Alignment Length: 941
• Identity: 173/941 - (18%)
• Similarity: 281/941 - (29%)
• Gaps: 359/941 - (38%)

  Fly  1285 TPGISATKEEDRLSNRGSI--------SSANSFASAHNYELFDFDGGAGGNESDCYQSCSSELTA 1341
            ||....:::|   |.|.||        ::.|||       |.|.|.|.
Yeast    13 TPEKRVSRQE---SQRKSIELPKLPPLNTRNSF-------LDDSDNGT----------------- 50

  Fly  1342 SGILEEDSVS----PIKDPETELDAATRAKFYQLLVDATLAEIEHSTHMDQTLGTDSEHHYESIR 1402
                  |::|    ||.|.:         :|...:..|.....:||...:   ||.|.   ||..
Yeast    51 ------DNISIGWTPISDTQ---------QFQSPVPQAFTFTSKHSARGN---GTSSS---ESTP 94

  Fly  1403 HSGDPIYEEIHEVPPPLPLSAPPLN-DAELEKKAARSIFEGASKYDILSYLVDAKERGLAREESF 1466
            .|...:.|.....||||..|...:. |:.:...:::|.....:|   ||::.:::||     ...
Yeast    95 KSTKYVKERRPPPPPPLLYSTESIRIDSPMVSPSSQSRERSPNK---LSFIGNSEER-----HHM 151

  Fly  1467 DY-GNNPKIIEEEAADTLS--------DLDKRQA----------EERER--------------DR 1498
            :| .|:.:|::...|:..|        |..|:||          .|||:              ..
Yeast   152 EYISNHSRILKSPFANGFSPNSPKSPRDSSKQQAHFSDESDLRCHEREKALPPIPFTTTLLLSPF 216

  Fly  1499 DSGQSSMSSLSPPP---HNFICGGAGKSSGSDVERQDSGVGSETSKTSRSKY---------QATP 1551
            |...|...:..|||   ...:.|.:..|...:....||......|...:|.:         :..|
Yeast   217 DDEDSEFFTKPPPPLSTSRNVSGNSRVSEALESVYSDSDYTFNNSNARQSSFNSLLGAKPLELAP 281

  Fly  1552 TVL-------------HLCEDCDGA-----------------------------------VEMQV 1568
            ::.             |....||..                                   :|.|:
Yeast   282 SITAPTQPFSIQSIDEHKLYQCDNVYKLSAIYEWILKVYFEWFNECVFTKIDLFQIVQLLLEFQM 346

  Fly  1569 GDN----------GILYAPLVCRKCGKKRVERKE-------------IITEIVE----TEEKYGR 1606
            ..|          ..:.|..:.:|..:..:...|             :.||::.    .:..||.
Yeast   347 PTNFDQDTIDSNVDNIMASFISQKAVRFDIINDEEVAVVVGGLDITGVFTELLPCYSFIDNTYGS 411

  Fly  1607 DLQII-LEEFCHPMLVAGLLTQEQLSAIF------------LNTEDLLENNQTLAERMRDALDM- 1657
            ...:| ....|.....:|...:.:||.|.            |..:||.|.|....:|.....|:
Yeast   412 TNSLICYSNVCTHGQSSGFRKEIKLSEIINKSVGLWTEYWHLTPDDLAEINPREVQRQSFIFDLI 476

  Fly  1658 SLEQGDDDLLTVNIGRIFLDFTQMLHAFESYCVRQAGASLL----------------LANLEKEK 1706
            .||:...::.|.              |.|.| .::...|||                |..|..|.
Yeast   477 ILEERSLNMATA--------------AVEIY-GKRFDKSLLPDEPEFKALAFDIFEPLIQLHTEF 526

  Fly  1707 ELLRIFLKV------------------SQMENAVL---------------RRMNLNSFL------ 1732
            .|..||.|:                  .:.:|..|               .:.|...|:
Yeast   527 LLTPIFWKLKTRGKFIDGVGKIYSKWCGEAKNIYLNYAKAMATVHEIIMWEKKNKTKFVTWLKEI 591

  Fly  1733 --MVPVQRVTKY----------------PLLLARLYKVTPSHLEGRELLK---QAQEKIELHLNH 1776
              .|.:.|...|                |:.|..:.|.|...:|..|.||   :..||:...:|.
Yeast   592 DNSVEITRSKMYHDVIFFGGFFKSLQNMPVTLRSILKNTDPSMEDYEYLKIVIKEVEKLNFEVNQ 656

  Fly  1777 INQEAKDVPTKLWR-----RISSSSPNRRASCEI---------DMINIKLRKMAIDVLEWNHDEV 1827
            ::..|.| ..||.|     .:|::|.|..:...:         |.|..||      .|...:.|.
Yeast   657 VHGLAID-HRKLVRFSKQLVLSTNSSNATSYVNVGGSTNANDDDAIQDKL------ALGLTYPER 714

  Fly  1828 RFAMEG-----RLLYTQPT-------------DSNWKKARTIKL------TPVNALLV------- 1861
            :..:.|     |.|:..||             .....|..|.|.      .|::.|.:
Yeast   715 KLVLSGTVYKKRDLWLDPTPVYIALLDNCLLITEEISKGETQKYKLIERPIPIDYLSLEKRKIPG 779

  Fly  1862 TNGKPSANYRAEKAMSEKLNFPKHTGIREASLLLVKEKCGRYTLIREPLYLDRCVVCSE-----A 1921
            |:.:|..||..::..|...||  .|.|.....||  :..|.:   ....|.||....:|     .
Yeast   780 TSKQPLRNYSQKEHKSPMHNF--STPINSMRPLL--KSSGNH---MSTAYGDRKTSNTEISNANP 837

  Fly  1922 DWDDY-FEVQEISSKDTFIFKAEDGVRTKQW 1951
            :.|:: |:::..::.::|.|..|......||
Yeast   838 NTDEFSFKIRNTATGESFKFFTESAEVLNQW 868

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF64C	NP_729032.1	RhoGEF	1590..1778	CDD:238091	52/294 (18%)
TUS1	NP_013529.3	RhoGEF	469..655	CDD:238091	34/200 (17%)
		CNH	939..1287	CDD:214481