Protein Alignment RhoGEF64C and itsn1

DIOPT Version :10

• Sequence 1: NP_729032.1
• Gene: RhoGEF64C / 38578
• FlyBaseID: FBgn0035574
• Length: 1984
• Species: Drosophila melanogaster
• Sequence 2: XP_002939390.1
• Gene: itsn1 / 733480
• XenbaseID: XB-GENE-5904194
• Length: 1709
• Species: Xenopus tropicalis
• Alignment Length: 1887
• Identity: 351/1887 - (18%)
• Similarity: 560/1887 - (29%)
• Gaps: 717/1887 - (37%)

  Fly   417 PRRPPRNKKADEPAEMPAAAGAAGAAGAAATS----PPAAAVTAATAVKPKAVTLAKVEKIEKDN 477
            |...|.| ...:|..|.|||.||.|||...:.    ||.|||  |....|
 Frog    95 PSALPSN-MLKQPVAMSAAAAAAAAAGFGMSGISGIPPLAAV--APVPMP--------------- 141

  Fly   478 ESAYTVLTVTPTSPAIKSILKRPTTLELPGVGMSPLPL-------PPVNNS--GLVTPAGKSQFY 533
                                      .:|.||||| ||       ||:.|.  .::.|
 Frog   142 --------------------------SIPVVGMSP-PLVSSVPTVPPLANGAPAVIQP------- 172

  Fly   534 IPTPTGTAATPTSSS-------TPMAT--------STPTPNASITATTAAAGAAITPGSRKKVQ- 582
            :|....:|..|.|||       :.|.|        ..|||       ...|..|:...||.|.:
 Frog   173 LPAFAHSATLPKSSSFGRSGAGSQMNTKLQKAQSFDVPTP-------PPLAEWAVPQSSRLKYRQ 230

  Fly   583 -FMVEDKIIATTATAATTDDSDASAVATKTNYEYYNRKRNAATATAAAATAASAAAASPPTAATS 646
             |...||.::...|.                                            |.|.|.
 Frog   231 LFNSHDKTMSGHLTG--------------------------------------------PQARTI 251

  Fly   647 TDQ------QEATLF-AADIDDEHNFTT--------------SGEAAPPFIKPDSLHYEDVLPGP 690
            ..|      |.||:: .:|||.:...|.              ||:..||.:.|:.:        |
 Frog   252 LMQSSLPQSQLATIWNLSDIDLDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYI--------P 308

  Fly   691 QIFDSSESSAKLSLPADGSPHVTTSPVSV---LPTGTSNGVGGAGLGPGPAGVSGEEAPATAAAT 752
            ..|....|.:.||:         .|.|||   ||                      |.||
 Frog   309 PSFRRVRSGSGLSI---------MSSVSVDQRLP----------------------EEPA----- 337

  Fly   753 AAATQKTTAANRDYDDDDEHNDK-------DESHDNKPNDLAEREQDREG-----DKEQGRAGEL 805
                          :::.::.||       |:..:|......|.|:.|:.     .|||.|..:|
 Frog   338 --------------EEEPQNTDKRLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQL 388

  Fly   806 GISRQEKKDAGGKPAASQKRAAQ------------TTQSRNPIRRAHSERHLSTAPLAAHGHASS 858
            ..:.||:|:   :....|:|..|            ..:.|...||...||..
 Frog   389 ERAEQERKE---RERQDQERKRQQELEKQLEKQRELERQREEERRKEIERRE------------- 437

  Fly   859 ACAAGRNCHSIPMTPPASGPQNVILNFK-----DTMALRIKSNR--------SDSR--------D 902
              ||.|..............:..:||.:     |.:.|:.|...        :|.:        |
 Frog   438 --AAKRELERQRQLEWERNRRQELLNQRNREQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQD 500

  Fly   903 LFQRRPTEPPPPPPPKGAGKGLEPLPHEINKSPLSPQRTVVRVAPFKPQQQQVGGS--------- 958
            :..|..|:                 .|||..:..|.:   :|:|.....|||:..|
 Frog   501 IRCRLTTQ-----------------RHEIESTNKSRE---LRIAEITHLQQQLQESQQLLGKLIP 545

  Fly   959 -----GDGF----THEVHR---LKISHSEEDADLG----------VELRRRPNL----------- 990
                 .|..    .:.:||   |.:..:.|..::|          ||...|..|
 Frog   546 EKQSLNDQLKQVQQNSLHRDSLLTLKRALEAKEMGRQQLRDQLDEVEKETRAKLQEIDVFNNQLK 610

  Fly   991 ----------------FLSSPGEEDAAPKKTSIL-----------ISGDDCYSTMNLSANAAQPL 1028
                            |.:...::....:|||.|           :..|..:.. .:.....:..
 Frog   611 ELRELYNRQQLQKQKDFEAEKIKQKELERKTSELDKMKEEDKRRMLEQDKLWQD-RVKQEEERYK 674

  Fly  1029 YQSSVVVNSHSATSVCISVSSAEEQEQELNQLNTLRSQQMGMG---------------------- 1071
            :|.........:...|......|.||:. |:|..|..:...:|
 Frog   675 FQDEEKQKREESAQQCEEEKKPEIQEKP-NKLFHLPPEPGKLGGHVPWMNTEKAPLTFNQGDVKV 738

  Fly  1072 ---IAIIPI--RGASEVNLQRSVSCISSTSSSSTSSSSSSSGRGRT-LIRLDY-DRERKGSATSS 1129
               .|:.|.  |...|:.:|.....:...|.:..........:|:| ....:| :|..:|...|:
 Frog   739 VYYRALYPFDARSHDEITIQPGDIIMVDESQTGEPGWLGGELKGKTGWFPANYAERMPEGEFPST 803

  Fly  1130 T----PINTPPVISSTPATPPPTDLPIENANPLVPHETELLKILRNPVEAVKLNLVPHVCGLRAP 1190
            |    .....|.:...|:||.|......:||.            .|..:          .....|
 Frog   804 TKQAADTTAKPTVHLAPSTPTPAAFTDNSANS------------NNWAD----------FSSTWP 846

  Fly  1191 TRESGKSKKIQEEEVSGTP--------------VKDPGFIAKLLQDPMLGQ--VAEGLETETVAE 1239
            |..:.|::....:.....|              ...|..:......|:|||  ..|||:.:.:..
 Frog   847 TNNTDKAETDNWDTWGAQPSLTVPSAGQHRQRSAFTPATVTGSSPSPVLGQGEKVEGLQAQALYP 911

  Fly  1240 LIENSLQRLQQARRGIDMSGTKDHDDM-----------------NRLISASLQRIRLERQPATPG 1287
            ........|...:.  |:....:..||                 .:|||..|::        :..
 Frog   912 WRAKKDNHLNFNKN--DIITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPLRK--------STS 966

  Fly  1288 ISATKEEDRLSNRGSISSANSFASAHNYE----LFDFDGGAGGN--------------ESDCYQS 1334
            |.:|..|...|.:...|.|  |..|...|    ::.::....|:              :.|.:..
 Frog   967 IDSTSSESPASLKRVSSPA--FKPAMQGEEYIAMYTYESNEQGDLTFQQGDLIVVIKKDGDWWTG 1029

  Fly  1335 CSSELTASGILEEDSVSPIKDPETELDAATRAKFYQLLVDATLAEIEHSTHMDQTLGTDSEHHYE 1399
            ...|.|  |:...:.|.| ||.|.   |.:..|...|.....:|::..|
 Frog  1030 TVGEKT--GVFPSNYVRP-KDSEA---AGSAGKTGSLGKKPEIAQVIAS---------------- 1072

  Fly  1400 SIRHSGDPIYEEIHEVPPPLPLSAPPLNDAELEKKAARSIFEGASKYDILSYLVDAKERGLAREE 1464
                         :....|..|:..|.....:.||.....:||           :.:.||..|:.
 Frog  1073 -------------YTATGPEQLTLAPGQLILIRKKNPGGWWEG-----------ELQARGKKRQI 1113

  Fly  1465 SFDYGNNPKIIEEEAADTLSDLDKRQAEERERDRDSGQSSMSSLSPPPHNFICGGAGKSSGSDVE 1529
            .:...|..|::                        |..::.|:.:.||         |::.....
 Frog  1114 GWFPANYVKLL------------------------SPGTNKSTPTEPP---------KATSLPPT 1145

  Fly  1530 RQDSG----VGSETSKTSRSKYQATPTVLHLCEDCD---GAVEMQVGDNGILYAPLVC------R 1581
            .|..|    :.....:.:..|.|.. .||:. ||.|   |.:...||.....|..|..      :
 Frog  1146 CQVIGMYDYIAQNDDELAFGKGQVI-NVLNK-EDPDWWKGELNGHVGLFPSNYVKLTTDMDPSQQ 1208

  Fly  1582 KCGKKRV-------ERKE--IITEIVETEEKYGRDLQIILEEFCHPMLVAGLLTQEQLSAIFLNT 1637
            .|....:       |||.  .|.|::.|||.|..|||::.|.|..|:|.:.|||:::::.||:|.
 Frog  1209 WCADLHLLDMLSPTERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESELLTEKEVAMIFVNW 1273

  Fly  1638 EDLLENNQTLAERMRDALDMSLEQGD----DDLLTVNIGRIFLDFTQMLHAFESYCVRQAGASLL 1698
            ::|:..|..|.:.:|....||.|:..    .|:||..:..        :..:..:|..|...:.|
 Frog  1274 KELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPH--------MQPYIRFCSCQLNGAAL 1330

  Fly  1699 LANLEKEKELLRIFLKVSQMENAVLRRMNLNSFLMVPVQRVTKYPLLLARLYKVTPSHLEGRELL 1763
            :.....|....:.|:|...|:.. .:.|.|:|||:.|:||||:|||::..:.:.||.:......|
 Frog  1331 IQQKTDEVPEFKEFVKRLAMDPR-CKGMPLSSFLLKPMQRVTRYPLIIKNIIENTPENHPDHSHL 1394

  Fly  1764 KQAQEKIELHLNHINQEAKDVPTKLWRRISSSSPNRRASCEIDMINIKLRKMAIDVLEWNHDEVR 1828
            |||.||.|...:.:|:..::                              |...|.|||....|:
 Frog  1395 KQALEKAEELCSQVNEGVRE------------------------------KENSDRLEWIQAHVQ 1429

  Fly  1829 FAMEG---RLLYTQPTD----------SNWKKARTIK-----LTPVNALLVTNGKPSANYRAEKA 1875
              .||   :|::...|:          ....||::.|     |.....||....||..:...:|.
 Frog  1430 --CEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGNDKV 1492

  Fly  1876 MSEKLNFPKHTGIREASLLLVKEKCGRYTLIREPLYLDRCVVCSEAD------------------ 1922
            .|.|.|.                   :|.:.:.|::|:..:|....|
 Frog  1493 FSPKSNL-------------------QYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYT 1538

  Fly  1923 -----------WDDYFEVQEISSKDTFIFKAEDGVRTKQWYTQLQYHAQGMGAWRKRRNALANIM 1976
                       |     ||:|.:......:.|...|.|.:..:.| .|.|:|          .:|
 Frog  1539 LRAESINERTAW-----VQKIKAASELYIETEKKKREKAYLVRSQ-RATGIG----------RLM 1587

  Fly  1977 IN 1978
            :|
 Frog  1588 VN 1589

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF64C	NP_729032.1	RhoGEF	1590..1778	CDD:238091	60/193 (31%)
itsn1	XP_002939390.1	EH	14..108	CDD:197477	4/13 (31%)
		EH	217..312	CDD:197477	25/146 (17%)
		PTZ00121	<354..701	CDD:173412	63/385 (16%)
		SH3_Intersectin1_1	740..794	CDD:212920	9/53 (17%)
		INTAP	794..905	CDD:435467	23/132 (17%)
		SH3_Intersectin1_2	905..956	CDD:212922	4/52 (8%)
		SH3_Intersectin1_3	994..1045	CDD:212924	6/52 (12%)
		SH3_Intersectin1_4	1062..1126	CDD:212926	14/127 (11%)
		SH3_Intersectin1_5	1146..1199	CDD:212928	13/54 (24%)
		RhoGEF	1226..1409	CDD:238091	58/191 (30%)
		PH_13	1428..1570	CDD:465218	27/167 (16%)
		C2_Intersectin	1570..1704	CDD:176021	7/31 (23%)