DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF64C and itsn2a

DIOPT Version :9

Sequence 1:	NP_001261425.1	Gene:	RhoGEF64C / 38578	FlyBaseID:	FBgn0035574	Length:	1984	Species:	Drosophila melanogaster
Sequence 2:	XP_021324162.1	Gene:	itsn2a / 566412	ZFINID:	ZDB-GENE-041014-84	Length:	1666	Species:	Danio rerio

Alignment Length:	1809	Identity:	342/1809 - (18%)
Similarity:	571/1809 - (31%)	Gaps:	642/1809 - (35%)

- Green bases have known domain annotations that are detailed below.

  Fly   479 SAYTVLTVTPTSPAIKSILKRPTTLELPGVGMSPLPLPPVNNSGLVTPAGKSQFYIPTPT----- 538
            |...:||..||.|.:..::..||.:.      .|||..|.:.|....|.|.:....|||.
Zfish   121 SVMPILTPIPTCPVMAPLVPVPTLIS------GPLPQMPNSISVPALPNGNATLLTPTPAFPVSS 179

  Fly   539 -----------GTAATPTSSSTPMATSTPTPNASITATTAAAGAAITPGSRKKVQ--FMVEDKII 590
                       |::::.:||:|.:|:::|        .|:::..|:...||.|.:  |...||::
Zfish   180 GLTKSHSLLDLGSSSSNSSSTTSLASNSP--------KTSSSDWAVPQSSRLKYRQLFNSLDKLM 236

  Fly   591 ATTATAATTDDSDASAVATKTNYEYYNRKRNAATATAAAATAASAAAASPPTAATSTDQQEATL- 654
                                :.|....:.|||.                  ||:..|..|.||:
Zfish   237 --------------------SGYLSGPQVRNAL------------------TASNLTQTQLATIW 263

  Fly   655 FAADIDDE------------H--NFTTSGE----AAPPFIKPDSLHYE---DVLPGPQIFDSSES 698
            |.||:|.:            |  ....:|.    ..||.:.|.||...   |:|.||....|:|
Zfish   264 FLADVDRDGQLRAEEFILAMHLVEMAKTGRPLPLTLPPDLVPPSLRTTKSCDLLNGPVPLISTE- 327

  Fly   699 SAKLSLPADGSPHVTTSPVSVLPTGTSNGVGGAGLGPGPAGVSGEEAPATAAATAAATQKTTAAN 763
                 |.....|....|.||.......|...|             .|.......|...|:.....
Zfish   328 -----LIEPELPQKNKSNVSYEDKLRENFQRG-------------NAELEKRRLALQEQQRREEE 374

  Fly   764 RDYDDDDEHNDKDESHDNKPNDLAERE-------QDREGDKEQGRAGELGISRQEK--KDAGGKP 819
            |..:::....:|.:..:.:..:..|||       :.||.::...|..||...|:|:  |:...:.
Zfish   375 RRREEERREEEKRQQKEREERERREREAREMEIRRQREEERRMERQRELERQREEERLKELERRE 439

  Fly   820 AASQKRAAQTTQSRNPIRRAHSERHLSTAPLAAHGHASSACAAGRNCHSIPMTPPASGPQNVILN 884
            .|.::|..:..:|:....:...|:.                                  ||.|..
Zfish   440 EAERQRRREWEKSKRVELQTQKEKE----------------------------------QNDIQR 470

  Fly   885 FKD-TMALRI---------KSNRSDSRDLFQRRPTEPPPPPPPKGAGKGLEPLPH-------EIN 932
            .|| ..:|.|         |...|..||...:|          :|....||.:..       :||
Zfish   471 LKDRKRSLEIELEAVGNKHKQISSQLRDAKSKR----------QGQWAELELINQRRDVCITDIN 525

  Fly   933 KSPLSPQRTVVRVAPFKPQQQQVGGSGDGFTHE-----VHRLKISHSEED-------ADLGVELR 985
            ...|.......::....|::|::......||..     ::.:|.|..|:|       ..|.| |.
Zfish   526 SLQLQFDEMQRQLNQLGPEKQRLADRLVHFTQNISSPLINDVKQSVCEKDRSCRKLKEQLDV-LE 589

  Fly   986 RRPNLFLSSPGEEDAAPKKTSILISGDDCYSTMNLSANAAQPLYQSSVVVNSHSATSVCIS---- 1046
            |.....||...:.:...|    .:..|||                   |:....:...|::
Zfish   590 RETTAKLSQMEQYNKEIK----FVEMDDC-------------------VLQGLLSLLGCLTNLFL 631

  Fly  1047 ----VSSAEEQEQE-LNQLNTLRSQQMGMGIAIIPIRGASEVNLQRSVSCISSTSSSSTSSSSSS 1106
                :...:.|:|: |.||..:||.::            .|:...|
Zfish   632 LIKELRQKQSQQQDVLEQLRRVRSDKL------------KELQRHR------------------- 665

  Fly  1107 SGRGRTLIRLDYDRERKGSATSSTPINTPPVISSTPATPPPTDLPIENANPLVPHETELLKILRN 1171
                    :.:.:|:||..                           |.|......|.|
Zfish   666 --------KEEEERKRKKE---------------------------EEAARQAQLEQE------- 688

  Fly  1172 PVEAVKLNLVPHVCGLRAPTRESGKSKKI-QEEEVSGTPVKDPGFIAKLLQDPMLGQVAEGLETE 1235
                            |....|:.:.:|: ||.|            |||.::.....:|..||.:
Zfish   689 ----------------RLEQEEAERQRKLAQERE------------AKLREEQQRQALARLLEAQ 725

  Fly  1236 TVAELIENSLQRLQQARRGIDMSGTKDHDDMNRLISASLQRIRLERQPAT---------PGISAT 1291
            ..|...|...:..::.|..:|:. |:.....|...:..||.:.:.....:         |..:..
Zfish   726 EQARAKEEKRRAEEKERNELDVH-TQSSLPFNSTENNILQPVVIPEVTCSSLTTYRALYPFTARN 789

  Fly  1292 KEEDRLSNRGSISSANSFASAHNYELFDFDGGAGGNESDCYQSCSSE-LTASGILEEDSVSP--- 1352
            .:|..|.....|....|......:....:.|..|.......:.||.: .|.....|.:|.:|
Zfish   790 SDELTLEADCFIQVDESTVRETGWLYGSYSGNRGWFPESYAERCSKDSQTQPTAAETESTAPSAN 854

  Fly  1353 --------IKDP-ETELDAATRAKFYQLLVDATLAEIEHSTHMDQTLG-----------TDSEHH 1397
                    |:.| .|....:|..:..|     .:|..|.|...|..||           ......
Zfish   855 YPGIPRVDIEGPTPTHTPVSTNTQHSQ-----AVALCEWSAKTDSHLGFCKDDIITVLEKQENWW 914

  Fly  1398 YESIRH------------------SGDPIYEEIHEVPPPLPLSAPPLNDAE-LEKKAARSIFEGA 1443
            |..::.                  ..:|:|..:.|:         .::|:. |::..|...:|..
Zfish   915 YGELKEKRGWFPCSHVSVVTTNNTQSEPLYSSVEEI---------EVSDSGLLDEYVALYTYESP 970

  Fly  1444 SKYDIL-----SYLVDAKE----RGLAREESFDYGNNPKIIEEEAADTLSD-LDKRQAEERERDR 1498
            ...|:.     ..||..||    ||...:::..:.:|  .::.:..||.|. :..::.|..:...
Zfish   971 ESGDLTFSAEDVVLVTEKEGEWWRGCIGDQTGLFPSN--YVKPKEPDTASSAVPSKKPEIAQVTT 1033

  Fly  1499 DSGQSSMSSLSPPPHNFI----------------CGGAGKSSG----SDVERQDSGVGSETSKTS 1543
            .|..::...||..|...|                ..|..:..|    |:|...::..|
Zfish  1034 VSPATTPEQLSLTPGQLIVVLHKNSNSWWLGELQARGKKRKKGWFQSSNVRLLEANSG------- 1091

  Fly  1544 RSKYQATPTVLHLCE-----DCDGA----VEMQVG--------DN------------GIL---YA 1576
                :.||....||:     |...|    :..|.|        ||            |:.   |.
Zfish  1092 ----KITPAPQPLCQVIAMYDYKAANKDEMSFQKGQLITVLNKDNPDWWKGEVAGVTGLFPTNYV 1152

  Fly  1577 PLVCR----------------KCGKKRVERKEIITEIVETEEKYGRDLQIILEEFCHPMLVAGLL 1625
            .:...                .|.::| :|:..|.|:::|||.|..||::.:|.|..||..:|.|
Zfish  1153 KMTTECDPSQQWCADLLSLDSMCTEER-KRQGYIHELIQTEESYLEDLELAVEVFYKPMAESGRL 1216

  Fly  1626 TQEQLSAIFLNTEDLLENNQTLAERMRDALDMSLEQGDDDLLTVNIGRIFLDFTQMLHAFESYCV 1690
            |:.::|.||:|..:|:    ..:.::..||.:..:...:.:....:|.|.......:.|:..:|.
Zfish  1217 TEAEISMIFVNWRELI----MCSTKLLKALRVRKKTAGERMPVQVVGDILSSELSHMQAYIRFCS 1277

  Fly  1691 RQAGASLLLANLEKEKEL-LRIFLKVSQMENAVLRRMNLNSFLMVPVQRVTKYPLLLARLYKVT- 1753
            .|..|:.||.. :.:|.| .::||| ....|...:.|.|:|||:.|:||:|:||||:..:.:.|
Zfish  1278 CQLNAAALLQQ-KTDKSLDFKLFLK-KIASNYRCKGMPLSSFLLKPMQRITRYPLLIKNILENTP 1340

  Fly  1754 PSHLEGREL---LKQAQE----------------KIELHLNHI---------------------- 1777
            |:|.:...|   |:||:|                ::|...||:
Zfish  1341 PAHADHSNLQAALEQAEELCSQVNEAVREKENSDRLEWIQNHVLCDGVIEHLVFNSLTNCLGPRK 1405

  Fly  1778 ---------NQEAKDVPTKLWRRI-------------SSSSP----NRRASCEIDM------INI 1810
                     .:.:|:    ||..:             .||||    |..::.:..|      :|.
Zfish  1406 LLHSGKLHKTKSSKE----LWALLFNDFLLLTYTSKQFSSSPDKLFNPNSNTQYKMYKTPVFLNE 1466

  Fly  1811 KLRKMAID------VLEWNH-DEVRFAMEGRLLYTQPTD-----SNW------------------ 1845
            .|.||..|      |...:| |.|         ||..||     :.|
Zfish  1467 VLVKMPSDPSSDDPVFHISHIDRV---------YTLKTDTINERTTWVQKIKAASDHFIETEKLK 1522

  Fly  1846 ----KKARTIKLTPVNALLVT------------NGKPSANYRAEKAMSEKLNFPKHTGIREASLL 1894
                .:||:.|.:.:..||||            |||  :|...|..|..:....:|    :...|
Zfish  1523 REKAYQARSQKNSGIGRLLVTVLEATELKPCKPNGK--SNPYCELTMGAQCYTSRH----QPDTL 1581

  Fly  1895 LVKEKCGRYTLIREPLYLDRCVVCSEADWDDYFEVQEISSKDTFIFKAEDGVRT 1948
            ..|........|:: ||.|  |:|......|.|      |.|.|:.:.|..|.|
Zfish  1582 NPKWSFNCQFFIKD-LYQD--VLCLTVFERDQF------SPDDFLGRTEVPVAT 1626

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF64C	NP_001261425.1	RhoGEF	1593..1779	CDD:279015	60/237 (25%)
itsn2a	XP_021324162.1	EH	7..92	CDD:197477
		EH	214..293	CDD:197477	22/116 (19%)
		SMC_N	453..>727	CDD:330553	67/442 (15%)
		SH3_Intersectin2_1	776..832	CDD:212921	7/55 (13%)
		SH3	881..932	CDD:327375	8/55 (15%)
		SH3_Intersectin_3	960..1011	CDD:212772	10/52 (19%)
		SH3_Intersectin2_4	1028..1086	CDD:212927	9/57 (16%)
		SH3	1101..1154	CDD:327375	9/52 (17%)
		RhoGEF	1181..1364	CDD:238091	58/188 (31%)
		PH_13	1383..1523	CDD:318790	23/152 (15%)
		C2_Intersectin	1524..1658	CDD:176021	30/118 (25%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.810