Protein Alignment RhoGEF64C and arhgef28a

DIOPT Version :9

• Sequence 1: NP_001261425.1
• Gene: RhoGEF64C / 38578
• FlyBaseID: FBgn0035574
• Length: 1984
• Species: Drosophila melanogaster
• Sequence 2: XP_017211758.2
• Gene: arhgef28a / 559941
• ZFINID: ZDB-GENE-060531-28
• Length: 1817
• Species: Danio rerio
• Alignment Length: 1393
• Identity: 266/1393 - (19%)
• Similarity: 455/1393 - (32%)
• Gaps: 447/1393 - (32%)

  Fly   786 LAEREQDREGDK--EQGRAGELGISRQEKKDAGGKPAASQK---------------RAAQTTQSR 833
            |||.||   |..  |||...:..:|..|:.|.....:..:|               :|.|..|..
Zfish   164 LAETEQ---GFNVCEQGNLSQSEVSGTEEFDPEQHSSVDEKVTQALANLHFLGWDGQAGQENQVL 225

  Fly   834 NP-----------------------------IRRAHSERHLSTAPLAAHG---------HASS-- 858
            .|                             :..|:.|.|...|....||         |.|:
Zfish   226 LPRETPLHVAMRLGFPHLSHFLLREFNGQMMVSIANEEGHTPVALAEKHGDPVLITALTHGSALR 290

  Fly   859 ---ACAAGRNCHS----IPMTPPASGPQNVILNFKDTMALRIKS----NRSDSRDLFQRRPTEPP 912
               .|....:|..    .|:|              |:::|.::.    |..:|..|::.:..||.
Zfish   291 PVWMCQIWTDCSQQLRICPVT--------------DSISLTVRHQTLINVQNSIQLYREKSKEPD 341

  Fly   913 PPPPPKGAGKGLEPLPH-------EINKSPLSPQRTVVRVAPFKPQQQQVGGSGDGFTHEVHRLK 970
            .....:...:..|...|       ::|.|.....:|:|                |....|  :|.
Zfish   342 ILTRVRHLSELSEGSVHRSDECDADLNNSLKIEDQTLV----------------DSVFEE--QLV 388

  Fly   971 ISHSEEDADLGVELRRRPNLFLSSPG--------EEDAAPKKTSILISGDDCYSTMNL------- 1020
            :...|||.||.|....:.   .||||        ...||..:.:.::.|....|.:|:
Zfish   389 LCLDEEDEDLSVFNSEKQ---FSSPGSCTPFHLHSSAAAAVRLTAMLKGSTKDSELNMKYAVPGA 450

  Fly  1021 ---SANAAQPLYQSSVVVNSHSATSVCISVSSAEEQEQELNQLNTLRSQQMGMGIAIIPIRGASE 1082
               |:.....:::.|....|...|   |.:|.|.:.::          .|.|...|.......|.
Zfish   451 TEDSSGTDSGMWRMSDTGVSSPGT---IDISPAVKDDE----------GQRGRHPAFRSFSPTSP 502

  Fly  1083 VNLQ--------RSVSCISSTSSSSTSSSSSSS---GRGRTLIRLDYDRE--------------- 1121
            ..|.        |:.....::|||.|.|....|   ....:|:.|:.|.|
Zfish   503 AGLMLDRFMRATRTTELTYNSSSSPTESCDLRSPILSLSPSLVALEMDSEEDDDDDILMKKPLPQ 567

  Fly  1122 ------------------------RKGSATSS---TPIN-------------TPPVISSTP---- 1142
                                    |..||:|:   .|..             |.|.||..|
Zfish   568 LQPDSKDSTDEQVMSSPAPVLICTRSHSASSACNPAPAKDVEQGVRLRSYSYTSPKISLLPPRFS 632

  Fly  1143 --ATPPPTDLPIENANPLVPHETELLKILRNPVEAVKLNLVPHVCGLRAPTRESGKSKKIQEEEV 1205
              |.|||.||..:...........||..|........||.|.|        |.|..|::.||:
Zfish   633 RDAHPPPADLSQDGVLSSSSGSKSLLHALSLSKSLSLLNPVKH--------RSSSTSEQPQEK-- 687

  Fly  1206 SGTPVKDPGFIAKLLQDPMLGQVAEGLETETVAELIENSLQRLQQARRGIDMSGTKDHDDMNRLI 1270
                           ::....:.|:..:.|:.|||.: |||.|                    .:
Zfish   688 ---------------RELRFRRRAQSADDESSAELAD-SLQHL--------------------TL 716

  Fly  1271 SASLQRIRLERQPATPGISATKEEDRLSNRGSISSANSFASAHNYELFDFDGGAG-GNESDCYQS 1334
            |..|:.|  |.:.....|..:|.|   |.:..:|...||..:..|...:.:.|.| ..|:...|.
Zfish   717 SEFLKEI--EEEEWDKYIIPSKTE---SEKYKVSRTFSFIKSRMYSTRNKNKGKGKEKETKDKQV 776

  Fly  1335 CSSELTASGILEEDSVSPIKDPETELDAATRAKFYQLLVDATLAEIEHSTHMDQTLGTDSEHHYE 1399
            ...:.||..:|..........|                  |:..|:.|.||...::       ::
Zfish   777 NGHQFTAGSVLGSTLCEICSKP------------------ASGKEVVHCTHCAVSV-------HK 816

  Fly  1400 SIRHSGDPIYEEIHEVPPPLPLSAPPLNDAELEKKAARSIFEGASKYDILSYLVDAKERGL---- 1460
            ..:.|..|..::..:                              ||.:::  |.:|...|
Zfish   817 GCKDSSTPCLKKPQD------------------------------KYSVMT--VKSKTASLPQNF 849

  Fly  1461 -AREESFD------YGNNPKIIEEEAADTLSDLDKRQAEERERDRDSGQSSMSSLSPPPHNFICG 1518
             .||.|..      ..:.|.:...::|.....|.:......||..:|..:  .|.||...|.|
Zfish   850 IVRESSSSSLQISTSASLPVMTSRDSAPLPCPLSRSVPLTTERFSESPDA--DSDSPLWRNHI-- 910

  Fly  1519 GAGKSSGSDVERQDSGVGSETSKTSRSKYQATPTVLHLCEDCDGAVEMQVGDNGILYAPLVCRKC 1583
                .|...::..:|...:::|....|  ..:|..:.|..:   ||:::.....:......||..
Zfish   911 ----QSEELLQTMESSTSTDSSIIEDS--VDSPLQVELLMN---AVDLEAESWSLAVEAQFCRMQ 966

  Fly  1584 GKKRVERKEIITEIVETEEKYGRDLQIILEEFCHPMLVAGLLTQEQLSAIFLNTEDLLENNQTLA 1648
            .|:.::|:::|.|.::||..:.:.|.::.|.|...|.....|..|.:..||...::||:.::...
Zfish   967 EKRIIKRQDVIYEFMQTELHHVQTLMVMAEVFRRGMRDEVGLDAETIGRIFPCLDELLQLHRDFL 1031

  Fly  1649 ERMRDALDMSLEQGDD-DLLTVNIGRIFL-DFTQ-----MLHAFESYCVRQAGASLLLANLEKEK 1706
            ..||:.....::.... :.|...:|.||| .|:|     |...:..:|.....|......|:::.
Zfish  1032 TAMRERRQSCVQNSSSKNFLIHRVGDIFLQQFSQENAEKMKQVYGEFCSHHTEAVSYFKELQQQN 1096

  Fly  1707 ELLRIFLKVSQMENAVLRRMNLNSFLMVPVQRVTKYPLLLARLYKVTPSHLEGRE---------- 1761
            :..::|:| .|..|::::|..:...:::..||:||||:||.|:.:.|.   ||.|
Zfish  1097 KRFQLFIK-QQSSNSLVKRREIPECILLVTQRITKYPVLLERILQYTD---EGTEEHSDLSRALV 1157

  Fly  1762 LLKQAQEKIELHLNHINQEAKDVPTKLWRRISSSSPNRRASCEIDMINIKLRKMAIDVLEWNHDE 1826
            |:::....::.:::...|..| :...|.|..:..|...:..|       ..||..|
Zfish  1158 LIRELISAVDQYVSQYEQNQK-LSEVLGRMENKCSTKLKTGC-------TFRKQDI--------- 1205

  Fly  1827 VRFAMEGR-LLYTQPTDSNWKKARTIKLTPVNALLVTNGKPSANYRAEKAMSEKLNFPKHTGIRE 1890
                ..|| ||:..|.  .||.| |.:|..|.|.|:::
Zfish  1206 ----TSGRNLLHQGPL--LWKTA-TGRLKDVLAFLLSD--------------------------- 1236

  Fly  1891 ASLLLVKEKCGRY---TLIREP--LYLDRCVVCSEADWDDYFEVQEISSKDTFIFKAEDGVRTKQ 1950
             ||:.::||..:|   |:.::|  :.|.:.:            |:|:::::..:|.........:
Zfish  1237 -SLVFLQEKDQKYIFATVDQKPPVISLQKLI------------VREVANEERGMFLISASSAGPE 1288

  Fly  1951 WYTQLQYHAQG---MGAW-RKRRNALAN 1974
            .|   :.||..   ..|| |..|.|:.|
Zfish  1289 MY---EVHAASKDERNAWMRLIREAVEN 1313

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF64C	NP_001261425.1	RhoGEF	1593..1779	CDD:279015	46/202 (23%)
arhgef28a	XP_017211758.2	ANK	<229..283	CDD:238125	6/53 (11%)
		C1	779..824	CDD:237996	10/69 (14%)
		RhoGEF	973..1167	CDD:238091	47/197 (24%)
		PH_p190RhoGEF	1211..1311	CDD:275430	29/145 (20%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	2.810