DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF64C and ITSN2

DIOPT Version :9

Sequence 1:	NP_001261425.1	Gene:	RhoGEF64C / 38578	FlyBaseID:	FBgn0035574	Length:	1984	Species:	Drosophila melanogaster
Sequence 2:	NP_006268.2	Gene:	ITSN2 / 50618	HGNCID:	6184	Length:	1697	Species:	Homo sapiens

Alignment Length:	1706	Identity:	329/1706 - (19%)
Similarity:	557/1706 - (32%)	Gaps:	537/1706 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly   488 PTSPA--IKSILKRPTTLELPGVGMSPLPL-PPVNNSGLVTPAGKSQFYIPTPTGTAATPTSSST 549
            |..||  |.|:....:...||.: |.|.|| |.|:.|.|             |.|||:.......
Human   130 PLPPAAPITSLSSATSGTNLPPL-MMPTPLVPSVSTSSL-------------PNGTASLIQPLPI 180

  Fly   550 PMATSTPTPNASITATTAAAGAAITPGSRKKVQFMVE-DKIIATTATAATTDDSDAS-----AVA 608
            |.::||....:|.:......|.|    |.:|.|.::: ....:|::||:.:.:|..:     ||.
Human   181 PYSSSTLPHGSSYSLMMGGFGGA----SIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVP 241

  Fly   609 TKTNYEY---YNRKRNAATATAAAATAASAAAASPPTAATSTDQQEATLFA-ADIDDEHNFTTSG 669
            ..|..:|   :|....:.:...:...|.:|...|     ..:..|.||::. ||:|.:...
Human   242 QPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQS-----NLSQTQLATIWTLADVDGDGQL---- 297

  Fly   670 EAAPPFIKPDSLHYEDVLPGPQIFDSSESSAKLSLPADGSPHVTTSPVSVLPTGTSNGVGGA--- 731
             .|..||.  ::|..|:             ||.     |.|...|.|..::|....   ||.
Human   298 -KAEEFIL--AMHLTDM-------------AKA-----GQPLPLTLPPELVPPSFR---GGKQID 338

  Fly   732 ---GLGPGPAGVSGEEAPATAAAT---------------------AAATQKTTAANRDYDDDDEH 772
               |..|....:..||.......|                     |...|:...|.|      :.
Human   339 SINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAER------KA 397

  Fly   773 NDKDESHDNKPNDLAERE--QDREGDKEQGRAGELGISRQE--KKDAGGKPAASQKRAAQTTQSR 833
            ..:.|..:.|..:|.|:|  :..|.:|...:..||...|:|  :||...:.||.|:...|.....
Human   398 QKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEW 462

  Fly   834 NPIRRAH--SERHLSTAPLAAHGHASSACAAGRNCHSIPMTPPASGPQNVILNFKDTMALRIKSN 896
            ..|||..  ::::.....:....      :..:|.|.          :...||.|..   :|...
Human   463 ERIRRQELLNQKNREQEEIVRLN------SKKKNLHL----------ELEALNGKHQ---QISGR 508

  Fly   897 RSDSRDLFQRRPTEPPPPPPPKGAGKGLEPLPHEINKSPLSPQRTVVRVAPFKPQQQQVGGSGDG 961
            ..|.|...|.:.||             ||.|..:.:          :.:...|..||::....:.
Human   509 LQDVRLKKQTQKTE-------------LEVLDKQCD----------LEIMEIKQLQQELQEYQNK 550

  Fly   962 FTHEV-------HRLKISHSEEDADLGVELRRRPNLFLSSPGEE---------DAAPKKTSILIS 1010
            ..:.|       .|:|........|.||.|..:.:|    ..||         ||..|:|:..:|
Human   551 LIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSL----EKEELCQRLKEQLDALEKETASKLS 611

  Fly  1011 GDDCYSTMNLSANAAQPLYQSSVVVNSHSATSVC-ISVSSAEEQEQELNQL--------NTLRSQ 1066
            ..|.::......|....:.|             | :|:.|.      ||.|        .|..:|
Human   612 EMDSFNNQLKCGNMDDSVLQ-------------CLLSLLSC------LNNLFLLLKELRETYNTQ 657

  Fly  1067 QMGMGIAIIPIRGASEVNLQRSVSCISSTSSSSTSSSSSSSGRGRTLIRLDYDRERKGSATSSTP 1131
            |:.:. .:..|:......::|.                          ||:..:::|
Human   658 QLALE-QLYKIKRDKLKEIERK--------------------------RLELMQKKK-------- 687

  Fly  1132 INTPPVISSTPATPPPTDLPIENANPLVPHETELLK-ILRNPVEAVKLNLVPHVCGLRAPTRESG 1195
                              |..|.|......:..|.| .||...|..:..|           :|..
Human   688 ------------------LEDEAARKAKQGKENLWKENLRKEEEEKQKRL-----------QEEK 723

  Fly  1196 KSKKIQEEE--VSGTPVKDPGFIAKLLQDPMLGQVAEGLETETVAELIENSLQRLQQARRGIDMS 1258
            ..:||||||  ......||...:.           ||..:.||.:.|:.         .|.:...
Human   724 TQEKIQEEERKAEEKQRKDKDTLK-----------AEEKKRETASVLVN---------YRALYPF 768

  Fly  1259 GTKDHDDMN-------------------------------------RLISASLQRIRLERQ---P 1283
            ..::||:|:                                     ::.|:..::....::   |
Human   769 EARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLP 833

  Fly  1284 ATPGISATKEEDRLSNRGSISSANSFASAHNYELFDFDGGAGGNESDCYQSCSSELTASGILEED 1348
            .|..:|||      |......|:|..||..:|:...| .....|.|...:|..:...:.|
Human   834 PTVSLSAT------STSSEPLSSNQPASVTDYQNVSF-SNLTVNTSWQKKSAFTRTVSPG----- 886

  Fly  1349 SVSPIKDPETELDAATRAKFYQLLVDATLAEIEH---STHMDQTLGTDSEHHYESIRHSGD---- 1406
            |||||.. :.::....:|   |.|...|..:..|   |.|...|:....|:.:....|.|.
Human   887 SVSPIHG-QGQVVENLKA---QALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFP 947

  Fly  1407 -------PIYEEIHEVPPPLPLSAPPLNDAELEKKAARSIFEGASKYDILSYLVDAKERGLAREE 1464
                   |..|...|.|..|        .|.:.||...:.:....:| |..|...:.|.|   :.
Human   948 KSYVKIIPGSEVKREEPEAL--------YAAVNKKPTSAAYSVGEEY-IALYPYSSVEPG---DL 1000

  Fly  1465 SFDYGNNPKIIEEEAADTLSDLDKRQA-----EERERDRDS-GQSSMSSLS-PPPHNFICGGAGK 1522
            :|..|....:.:::.......:..|..     ..:.:|::| |.:|.|..| ..|.......|..
Human  1001 TFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYV 1065

  Fly  1523 SSGSD------------VERQDSG------------------VGSETSKTSRSKYQATPTVLHLC 1557
            :|||:            :::..||                  ..|.......|..:|||....:|
Human  1066 ASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVC 1130

  Fly  1558 E---------DCDGAVEMQVGD-----------------NGIL------YAPLVC------RKCG 1584
            :         :.:..:....|.                 ||:.      |..:..      :.|.
Human  1131 QVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCA 1195

  Fly  1585 -------KKRVERKE--IITEIVETEEKYGRDLQIILEEFCHPMLVAGLLTQEQLSAIFLNTEDL 1640
                   .:.:|||.  .|.|:::|||:|..|||:::|.|...|..:|.||:.:::.||:|.::|
Human  1196 DLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKEL 1260

  Fly  1641 LENNQTLAERMRDALDMSLEQGDDDLLTVNIGRIFLDFTQMLHAFESYCVRQA-GASLLLANLEK 1704
            :.:|..|.:    ||.:..:.|.:.:....||.|.......:.|:..:|..|. ||:||....::
Human  1261 IMSNTKLLK----ALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDE 1321

  Fly  1705 EKELLRIFLKVSQMENAVLRRMNLNSFLMVPVQRVTKYPLLLARLYKVTPSHLEGRELLKQAQEK 1769
            :.:......|::  .:...:.|.|:|||:.|:||:|:||||:..:.:.||........||.|.|:
Human  1322 DTDFKEFLKKLA--SDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALER 1384

  Fly  1770 IELHLNHINQEAKDVPTKLWRRISSSSPNRRASCEIDMINIKLRKMAIDVLEWNHDEVRFAMEG- 1833
            .|...:.:|:..::                              |...|.|||....|:  .||
Human  1385 AEELCSQVNEGVRE------------------------------KENSDRLEWIQAHVQ--CEGL 1417

  Fly  1834 --RLLYTQPTD--------SNWKKART--------------IKLT-PVNALLVTNGKPSANYRAE 1873
              :|::...|:        .:.|..:|              :.|| .|....|::|       :|
Human  1418 AEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSG-------SE 1475

  Fly  1874 KAMSEKLNFPKHTGIREASLLLVKEKCGRYTLIREPLYLDRCVVCSEADWDDYFEVQEISSKDTF 1938
            |..|.|.|                   .::.:.:.|::|:..:|....|......|..||..|..
Human  1476 KLFSSKSN-------------------AQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRV 1521

  Fly  1939 IFKAEDGVRTKQWYTQ 1954
            .....|.:..:..:.|
Human  1522 YTLRTDNINERTAWVQ 1537

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF64C	NP_001261425.1	RhoGEF	1593..1779	CDD:279015	55/186 (30%)
ITSN2	NP_006268.2	SH3_Intersectin2_4	1057..1115	CDD:212927	7/57 (12%)
		SH3_Intersectin2_5	1130..1183	CDD:212929	5/52 (10%)
		RhoGEF	1214..1394	CDD:214619	55/185 (30%)
		PH_13	1412..1554	CDD:318790	27/154 (18%)
		C2_Intersectin	1555..1689	CDD:176021
		EH	15..107	CDD:197477
		SOBP	<90..182	CDD:317654	19/65 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	219..241		5/21 (24%)
		EH	237..316	CDD:197477	21/108 (19%)
		Neuromodulin_N	<346..756	CDD:331332	97/555 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	350..378		3/27 (11%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	691..752		19/82 (23%)
		SH3_Intersectin2_1	759..815	CDD:212921	5/64 (8%)
		INTAP	815..902	CDD:318758	22/99 (22%)
		SH3_Intersectin2_2	902..953	CDD:212923	11/53 (21%)
		SH3_Intersectin2_3	985..1036	CDD:212925	8/54 (15%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	87	1.000	Domainoid score	I8015
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	3	2.810