Protein Alignment RhoGEF64C and ITSN2

DIOPT Version :10

• Sequence 1: NP_729032.1
• Gene: RhoGEF64C / 38578
• FlyBaseID: FBgn0035574
• Length: 1984
• Species: Drosophila melanogaster
• Sequence 2: NP_006268.2
• Gene: ITSN2 / 50618
• HGNCID: 6184
• Length: 1697
• Species: Homo sapiens
• Alignment Length: 1700
• Identity: 328/1700 - (19%)
• Similarity: 556/1700 - (32%)
• Gaps: 525/1700 - (30%)

  Fly   488 PTSPA--IKSILKRPTTLELPGVGMSPLPL-PPVNNSGLVTPAGKSQFYIPTPTGTAATPTSSST 549
            |..||  |.|:....:...||.: |.|.|| |.|:.|.|             |.|||:.......
Human   130 PLPPAAPITSLSSATSGTNLPPL-MMPTPLVPSVSTSSL-------------PNGTASLIQPLPI 180

  Fly   550 PMATSTPTPNASITATTAAAGAAITPGSRKKVQFMVE-DKIIATTATAATTDDSDAS-----AVA 608
            |.::||....:|.:......|.|    |.:|.|.::: ....:|::||:.:.:|..:     ||.
Human   181 PYSSSTLPHGSSYSLMMGGFGGA----SIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVP 241

  Fly   609 TKTNYEY---YNRKRNAATATAAAATAASAAAASPPTAATSTDQQEATLFA-ADIDDEHNFTTSG 669
            ..|..:|   :|....:.:...:...|.:|...|     ..:..|.||::. ||:|.:...
Human   242 QPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQS-----NLSQTQLATIWTLADVDGDGQL---- 297

  Fly   670 EAAPPFIKPDSLHYEDVLPGPQIFDSSESSAKLSLPADGSPHVTTSPVSVLPTGTSNGVGGA--- 731
             .|..||.  ::|..|:             ||.     |.|...|.|..::|....   ||.
Human   298 -KAEEFIL--AMHLTDM-------------AKA-----GQPLPLTLPPELVPPSFR---GGKQID 338

  Fly   732 ---GLGPGPAGVSGEEAPATAAAT---------------------AAATQKTTAANRDYDDDDEH 772
               |..|....:..||.......|                     |...|:...|.|      :.
Human   339 SINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAER------KA 397

  Fly   773 NDKDESHDNKPNDLAERE--QDREGDKEQGRAGELGISRQE--KKDAGGKPAASQKRAAQTTQSR 833
            ..:.|..:.|..:|.|:|  :..|.:|...:..||...|:|  :||...:.||.|:...|.....
Human   398 QKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEW 462

  Fly   834 NPIRRAH--SERHLSTAPLAAHGHASSACAAGRNCHSIPMTPPASGPQNVILNFKDTMALRIKSN 896
            ..|||..  ::::.....:....      :..:|.|.          :...||.|..   :|...
Human   463 ERIRRQELLNQKNREQEEIVRLN------SKKKNLHL----------ELEALNGKHQ---QISGR 508

  Fly   897 RSDSRDLFQRRPTEPPPPPPPKGAGKGLEPLPHEINKSPLSPQRTVVRVAPFKPQQQQVGGSGDG 961
            ..|.|...|.:.||             ||.|..:.:          :.:...|..||::....:.
Human   509 LQDVRLKKQTQKTE-------------LEVLDKQCD----------LEIMEIKQLQQELQEYQNK 550

  Fly   962 FTHEV-------HRLKISHSEEDADLGVELRRRPNLFLSSPGEE---------DAAPKKTSILIS 1010
            ..:.|       .|:|........|.||.|..:.:|    ..||         ||..|:|:..:|
Human   551 LIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSL----EKEELCQRLKEQLDALEKETASKLS 611

  Fly  1011 GDDCYSTMNLSANAAQPLYQSSVVVNSHSATSVC-ISVSSAEEQEQELNQL--------NTLRSQ 1066
            ..|.::......|....:.|             | :|:.|.      ||.|        .|..:|
Human   612 EMDSFNNQLKCGNMDDSVLQ-------------CLLSLLSC------LNNLFLLLKELRETYNTQ 657

  Fly  1067 QMGMGIAIIPIRGASEVNLQRSVSCISSTSSSSTSSSSSSSGRGRTLIRLDYDRERKGSATSSTP 1131
            |:.:. .:..|:......::|.                          ||:..:::|
Human   658 QLALE-QLYKIKRDKLKEIERK--------------------------RLELMQKKK-------- 687

  Fly  1132 INTPPVISSTPATPPPTDLPIENANPLVPHETELLK-ILRNPVEAVKLNLVPHVCGLRAPTRESG 1195
                              |..|.|......:..|.| .||...|..:..|           :|..
Human   688 ------------------LEDEAARKAKQGKENLWKENLRKEEEEKQKRL-----------QEEK 723

  Fly  1196 KSKKIQEEE--VSGTPVKDPGFIAKLLQDPMLGQVAEGLETETVAELIENSLQRLQQARRGIDMS 1258
            ..:||||||  ......||...:.           ||..:.||.:.|:.         .|.:...
Human   724 TQEKIQEEERKAEEKQRKDKDTLK-----------AEEKKRETASVLVN---------YRALYPF 768

  Fly  1259 GTKDHDDMN----RLISASLQRI--------------------RLERQPATPGISATKEEDRL-- 1297
            ..::||:|:    .:|....:.:                    .:|:.|::....|...:..|
Human   769 EARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLP 833

  Fly  1298 --------SNRGSISSANSFASAHNYELFDFDGGAGGNESDCYQSCSSELTASGILEEDSVSPIK 1354
                    |......|:|..||..:|:...| .....|.|...:|..:...:.|     |||||.
Human   834 PTVSLSATSTSSEPLSSNQPASVTDYQNVSF-SNLTVNTSWQKKSAFTRTVSPG-----SVSPIH 892

  Fly  1355 DPETELDAATRAKFYQLLVDATLAEIEH---STHMDQTLGTDSEHHYESIRHSGD---------- 1406
            . :.::....:|   |.|...|..:..|   |.|...|:....|:.:....|.|.
Human   893 G-QGQVVENLKA---QALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKI 953

  Fly  1407 -PIYEEIHEVPPPLPLSAPPLNDAELEKKAARSIFEGASKYDILSYLVDAKERGLAREESFDYGN 1470
             |..|...|.|..|        .|.:.||...:.:....:| |..|...:.|.|   :.:|..|.
Human   954 IPGSEVKREEPEAL--------YAAVNKKPTSAAYSVGEEY-IALYPYSSVEPG---DLTFTEGE 1006

  Fly  1471 NPKIIEEEAADTLSDLDKRQA-----EERERDRDS-GQSSMSSLS-PPPHNFICGGAGKSSGSD- 1527
            ...:.:::.......:..|..     ..:.:|::| |.:|.|..| ..|.......|..:|||:
Human  1007 EILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQ 1071

  Fly  1528 -----------VERQDSG------------------VGSETSKTSRSKYQATPTVLHLCE----- 1558
                       :::..||                  ..|.......|..:|||....:|:
Human  1072 LSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMY 1136

  Fly  1559 ----DCDGAVEMQVGD-----------------NGIL------YAPLVC------RKCG------ 1584
                :.:..:....|.                 ||:.      |..:..      :.|.
Human  1137 DYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLD 1201

  Fly  1585 -KKRVERKE--IITEIVETEEKYGRDLQIILEEFCHPMLVAGLLTQEQLSAIFLNTEDLLENNQT 1646
             .:.:|||.  .|.|:::|||:|..|||:::|.|...|..:|.||:.:::.||:|.::|:.:|..
Human  1202 TMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTK 1266

  Fly  1647 LAERMRDALDMSLEQGDDDLLTVNIGRIFLDFTQMLHAFESYCVRQA-GASLLLANLEKEKELLR 1710
            |.:    ||.:..:.|.:.:....||.|.......:.|:..:|..|. ||:||....:::.:...
Human  1267 LLK----ALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKE 1327

  Fly  1711 IFLKVSQMENAVLRRMNLNSFLMVPVQRVTKYPLLLARLYKVTPSHLEGRELLKQAQEKIELHLN 1775
            ...|::  .:...:.|.|:|||:.|:||:|:||||:..:.:.||........||.|.|:.|...:
Human  1328 FLKKLA--SDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCS 1390

  Fly  1776 HINQEAKDVPTKLWRRISSSSPNRRASCEIDMINIKLRKMAIDVLEWNHDEVRFAMEG---RLLY 1837
            .:|:..::                              |...|.|||....|:  .||   :|::
Human  1391 QVNEGVRE------------------------------KENSDRLEWIQAHVQ--CEGLAEQLIF 1423

  Fly  1838 TQPTD--------SNWKKART--------------IKLT-PVNALLVTNGKPSANYRAEKAMSEK 1879
            ...|:        .:.|..:|              :.|| .|....|::|       :||..|.|
Human  1424 NSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSG-------SEKLFSSK 1481

  Fly  1880 LNFPKHTGIREASLLLVKEKCGRYTLIREPLYLDRCVVCSEADWDDYFEVQEISSKDTFIFKAED 1944
            .|                   .::.:.:.|::|:..:|....|......|..||..|.......|
Human  1482 SN-------------------AQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTD 1527

  Fly  1945 GVRTKQWYTQ 1954
            .:..:..:.|
Human  1528 NINERTAWVQ 1537

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF64C	NP_729032.1	RhoGEF	1590..1778	CDD:238091	57/190 (30%)
ITSN2	NP_006268.2	EH	15..107	CDD:197477
		Atrophin-1	<86..>195	CDD:460830	23/78 (29%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	219..241		5/21 (24%)
		EH	237..316	CDD:197477	21/108 (19%)
		PTZ00121	<346..756	CDD:173412	97/555 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	350..378		3/27 (11%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	691..752		19/82 (23%)
		SH3_Intersectin2_1	759..815	CDD:212921	6/64 (9%)
		SH3_Intersectin2_2	902..953	CDD:212923	11/53 (21%)
		SH3_Intersectin2_3	985..1036	CDD:212925	8/54 (15%)
		SH3_Intersectin2_4	1057..1115	CDD:212927	7/57 (12%)
		SH3_Intersectin2_5	1130..1183	CDD:212929	5/52 (10%)
		RhoGEF	1214..1394	CDD:214619	55/185 (30%)
		PH_13	1412..1555	CDD:465218	26/153 (17%)
		C2_Intersectin	1555..1689	CDD:176021