DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF64C and RhoGEF2

DIOPT Version :9

Sequence 1:	NP_001261425.1	Gene:	RhoGEF64C / 38578	FlyBaseID:	FBgn0035574	Length:	1984	Species:	Drosophila melanogaster
Sequence 2:	NP_477317.1	Gene:	RhoGEF2 / 36915	FlyBaseID:	FBgn0023172	Length:	2559	Species:	Drosophila melanogaster

Alignment Length:	2121	Identity:	393/2121 - (18%)
Similarity:	673/2121 - (31%)	Gaps:	694/2121 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly   218 PSI----LDPQKD--PYDLMRRTRLLSPEPSSKEADSKSRSRSETVANQPRGRTRTRAP-----P 271
            |||    ||...|  .||.::..    ||.||.:....|.|.:.|..:...|......|     .
  Fly     4 PSIKKRLLDLYTDEHEYDEVQEI----PEESSIQPPETSTSHTSTNGSSHSGPGTATGPGATSAG 64

  Fly   272 PPAPIQKSPAVAVPTA--VPDPTDCWLMEDNESLLQSSGLASRANSRRSILRCHVNPYDLISSEN 334
            |.|...:||.:.|.:.  :|.|.. ..:::::|..:...||:::...||         ..::|..
  Fly    65 PSAGAPQSPVIVVDSVPELPAPKQ-KSVKNSKSKQKQKQLANKSKIPRS---------PSLASSL 119

  Fly   335 GKLHSNLRKPNDHFDVADAQLNDDELGERKSKGTSKFY-----SGNVAAIAGQRISLGDKSGDSS 394
            ..|.|:|....|.....|....:......::......|     :|:..|.||..:|.......::
  Fly   120 SSLASSLSGHRDRDKDRDKDRENQNAVPPQTPPLPPSYKQNQMNGDSTAAAGGGVSAPATPTTAN 184

  Fly   395 DPGDSYERIIMVSGG----ESTASNGPRRPPRNKKADEPAEMPAAAGAAGAAGAAATSPPAAAVT 455
            :...|:....::.||    :|..||...:.|..:.:....  |.:...:|.....:|:|.....|
  Fly   185 NNNASHNNGSIMGGGVQLNQSDNSNPVLQAPGERSSLNLT--PLSRDLSGGHTQESTTPATTPST 247

  Fly   456 AATAVKPK-----AVTLAKVE-----KIEKDNESAYTVLTVTP---------------------- 488
            .:.|: ||     .:|:.|..     |:..||  ...|.:|.|
  Fly   248 PSLAL-PKNFQYLTLTVRKDSNGYGMKVSGDN--PVFVESVKPGGAAEIAGLVAGDMILRVNGHE 309

  Fly   489 ----TSPAIKSILKRPTTLELPGVGMSPLPLPPVNNSGLVTPAGKSQFYIPTPTGTAATPTSSST 549
                ..|.:..::|..||:||.......|..|  ::..:|||:        ||..:....|:|.|
  Fly   310 VRLEKHPTVVGLIKASTTVELAVKRSQKLTRP--SSVSVVTPS--------TPILSGRDRTASIT 364

  Fly   550 PMATSTPTPNASITATTAAAGAAITPGSRKKVQFMVEDKIIATTATAATTDDSDASAVATKTNYE 614
                 .|.|..||...........|      :|.|:|.:.:  ......:|.::.|...::.|..
  Fly   365 -----GPQPVDSIKRREMETYKIQT------LQKMLEQEKL--NLERLKSDQNNPSYKLSEANIR 416

  Fly   615 YYNRKRNAATATAAAATAASAAAASPPTAATSTDQQEATLFAADIDDEHNFTTSGEAAPPFIKPD 679
            ....:.:...|..|......|||.:..||..:.:|.:....:|        |.|.:.........
  Fly   417 KLREQLHQVGAEDAPTVKLQAAAGNKNTALLTPNQIQHLSASA--------THSNQQFHHLHHHH 473

  Fly   680 SLHYEDVLPGPQIFDSSESSAKLSLPADGSPHVTTSPVSVLPTGTSNGVGGAGLG---------- 734
            :||..:..|..|             ||..||    :.:|:||...|:    ..||
  Fly   474 NLHNNNYPPQQQ-------------PASTSP----AFLSLLPRSLSS----LSLGTRKNKTEKDL 517

  Fly   735 --PGPAGVS---------GEEAPATAAATAAATQKTTAANRDYDDDDEHNDKDESHDNK------ 782
              ..|.|::         ..:|.:.:.:....|...|:....:....:|..|:....:|
  Fly   518 TTSSPFGLTTDFLQQQRMSHQAESMSQSMHQHTSTPTSQQFFHPHQQQHRFKETGPTSKGKNKFL 582

  Fly   783 ----------PNDLAEREQDRE---------------------GDKEQGRAGELGISRQEKKDAG 816
                      |..|.:|...|:                     .|.::..:.:|..|:|::
  Fly   583 ISRSLIEEDVPPPLPQRNPPRQLNLDLKNGNASPGGSHLVAPVSDLDRATSPQLNRSQQQQ---- 643

  Fly   817 GKPAASQKRAAQTTQSRNPIR-RAHSERHLSTAPLAAHGHASSACAAGRNCHSIPMTPPASGPQN 880
             .|:::....:.....|:.|: :|.|:..:||........||:|.|||.
  Fly   644 -LPSSTDNSPSNAKSKRSKIKTKALSDPKMSTQMFLQMESASAAGAAGG---------------- 691

  Fly   881 VILNFKDTMALRIKSNRSDSRDLFQRRPTEPPPPPPPKGAGKGLEPL----------PHEINKS- 934
                     ::.:...               |||.||:..|...|.:          |:.:..:
  Fly   692 ---------SIEVDGG---------------PPPLPPRLPGMMTEDMSRGSCQNLAQPNSVGTAF 732

  Fly   935 --------------------PLSPQRTVVRVAPFKPQQQQ------------------------- 954
                                |||.:..:|:......||||
  Fly   733 NYPLVSTTTAVQNDNLNIAFPLSQRPNIVQQLQQYQQQQQHQMSGGQATGALGQTPNLGKNKHRR 797

  Fly   955 VGGSGDGFTHEVHRLKISHS--------EEDADLGVELRRRPNLFLSSPGEEDAAPKKTSILISG 1011
            ||.|.|.. |..|..:|:.:        |:|.:             |||......|..:|..::.
  Fly   798 VGSSPDNM-HPRHPDRITKTTSGSWEIVEKDGE-------------SSPPGTPPPPYLSSSHMTV 848

  Fly  1012 DDCYSTMNLSANAAQPLYQSSVVVNSHSATSVC-----ISVS-------------------SAEE 1052
            .:..:..|..|.||.|    .|.:.||..|.:.     |.:|                   |.|:
  Fly   849 LEDPNENNRGAAAAGP----GVFIESHQFTPMAGASSPIPISLHSSHMHAAQSNDTQKEIISMED 909

  Fly  1053 QEQEL-----------NQLNTLRSQQMGMGIAII-----------PI------------------ 1077
            :..:|           |.|..|...:....:||.           |:
  Fly   910 ENSDLDEPFIDENGPFNNLTRLLEAENVTFLAIFLNYVISNSDPAPLLFYLITELYKEGTSKDMR 974

  Fly  1078 RGASEVNLQRSVSCISSTSSSSTSSSSSSSGRGRTLIRLDYD----------RERK--------- 1123
            :.|.|::   |...:.....|......|.:.....:::|:||          |.||
  Fly   975 KWAYEIH---STFLVPRAPLSWYRQDESLAREVDNVLQLEYDKVEILRTVFLRSRKRAKDLISEQ 1036

  Fly  1124 ----------GSATSSTPIN---------------------------------TPP------VIS 1139
                      |..|...|.:                                 :||      .:.
  Fly  1037 LREFQQKRTAGLGTIYGPTDDKLAEAKTDKLREQIIDKYLMPNLHALIEDENGSPPEDVRKVALC 1101

  Fly  1140 STPAT---------PPPTDLPIENANPLVPHETELLK----------------ILRNPVEAVKLN 1179
            |..:|         |||:.: :|..:..|..:.....                :||...|....|
  Fly  1102 SALSTVIYRIFNTRPPPSSI-VERVHHFVSRDKSFKSRIMGKNRKMNVRGHPLVLRQYYEVTHCN 1165

  Fly  1180 LVPHV-------------CGLRAPTRESGKSKKIQEEEVSGTPVKDPGFIAKLLQDPMLGQVAEG 1231
            ....:             |.|....:.|    |:.:|...| |:..   ..:|..:..:.:....
  Fly  1166 HCQTIIWGVSPQGYHCTDCKLNIHRQCS----KVVDESCPG-PLPQ---AKRLAHNDKISKFMGK 1222

  Fly  1232 LETETVAELIENSLQRLQQARRGIDMSGTKDHDDMNRLISASLQRIRLERQPATPGISATKEEDR 1296
            :...| :::|.|..:..|...  :|:..|.|..      .||:.|...:|:| ...||.....:.
  Fly  1223 IRPRT-SDVIGNEKRSRQDEE--LDVELTPDRG------QASIVRQPSDRRP-DANISIRSNGNT 1277

  Fly  1297 LSNRGSISSANSFASAHN---YELFDFDGGAGGNESDCYQSCSSELTASGILEEDSVSPIKDPET 1358
            ..|...:::.:..:|.|.   .:..:..||||     |....|:.:.::......||:.......
  Fly  1278 SCNTSGLNTTDLQSSFHGSCANDSINPGGGAG-----CNMDLSTSVASTTPSTSGSVAAGLSAFA 1337

  Fly  1359 ELDA-------ATRAKFYQLLVDATLAEIEHSTHMDQTLGTDSEHHYE----------SIRHSGD 1406
            ||:|       |.|.::.|           |..|....:..:....|:          |.|.:.|
  Fly  1338 ELNALDTVDKEARRERYSQ-----------HPKHKSAPVSVNRSESYKERLSNKRNRNSRRKTSD 1391

  Fly  1407 PIYEEIHEVPPPLPLSAPPLNDAELEKKAARSIFEGASKYDILSYLVDAKERGLAREESF----- 1466
            |            .||:.| ||.:|:...:.:.:.|:|...:.|  ....|......|.|
  Fly  1392 P------------SLSSRP-NDEQLDLGLSNATYVGSSNSSLSS--AGGSESPSTSMEHFAAPGA 1441

  Fly  1467 -----------DYGNNPKIIEEEAADTLSDLDKRQAEERERDRDSGQSSMSSLSPPPHNFICGGA 1520
                       :...:|.::.::.|......|..||.                       :.|.|
  Fly  1442 AGGVQVPPMGLNQNQHPHLLIQQHAQQYCQQDSFQAG-----------------------LAGAA 1483

  Fly  1521 GKSSGSDVERQDSGVGSETSKTSRSKYQATPTVLHLCEDCDGAVEMQVGDNGILYAPLVCRKCGK 1585
            |.|:.|:....::|.....::.:...           ||.|   ::...|...:.|..|......
  Fly  1484 GSSAASNSSFWNAGHPLPVARWTLES-----------EDED---DVNEADWSSMVAAEVLAALTD 1534

  Fly  1586 KRVERKEIITEIVETEEKYGRDLQIILEEFCHPMLVAGLLTQEQLSAIFLNTEDLLENNQTLAER 1650
            ...:|:|||.||.:||..:.|.|:::...|..|:..:|||:|:.|..:|  ...||    :|.|
  Fly  1535 AEKKRQEIINEIYQTERNHVRTLKLLDRLFFLPLYESGLLSQDHLLLLF--PPALL----SLRE- 1592

  Fly  1651 MRDALDMSLEQ------------GD--DDLLTVNIGRIFLDFTQMLHAFESYCVRQAGASLLLAN 1701
            :..|.:.||:|            ||  .|:.....|.:..:|.      ..:|.||..|...|..
  Fly  1593 IHGAFEQSLKQRRIEHNHVVNTIGDLLADMFDGQSGVVLCEFA------AQFCARQQIALEALKE 1651

  Fly  1702 LEKEKELLRIFLKVSQMENAVLRRMNLNSFLMVPVQRVTKYPLLLARLYKVT----PSHLEGREL 1762
            ...:.|:|:..||.|:...| .||:.|...|...:||:||||||...|||||    |.:....|.
  Fly  1652 KRNKDEMLQKLLKKSESHKA-CRRLELKDLLPTVLQRLTKYPLLFENLYKVTVRLLPENTTEAEA 1715

  Fly  1763 LKQAQEKIELHLNHINQEAKDVP-----TKLWRRISSSSPNRRASCEIDMI--------NIKLRK 1814
            :::|.|..:..|..:||..|...     ..:.|::..||.::....::|:.        |:.::|
  Fly  1716 IQRAVESSKRILVEVNQAVKTAEDAHKLQNIQRKLDRSSYDKEEFKKLDLTQHHLIHDGNLTIKK 1780

  Fly  1815 MAIDVLEWNHDEVRFAMEGRL------LYTQPTDSNWKKARTIKLTPVNALLVTNGKPSANYRAE 1873
                     :..|:  :.|.|      |.|:..|..:.|...   ||   |.:||
  Fly  1781 ---------NPSVQ--LHGLLFENMIVLLTKQDDKYYLKNLH---TP---LSITN---------- 1818

  Fly  1874 KAMSEKLNFPKHTGIREA-----SLLLVKEKCGRYTLIREP 1909
            |.:|..::....|.||:.     |..|:|.|..:...:|.|
  Fly  1819 KPVSPIMSIDADTLIRQEAADKNSFFLIKMKTSQMLELRAP 1859

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF64C	NP_001261425.1	RhoGEF	1593..1779	CDD:279015	62/203 (31%)
RhoGEF2	NP_477317.1	PDZ	260..334	CDD:214570	14/75 (19%)
		RGS_GEF_like	934..1055	CDD:188710	16/123 (13%)
		C1_1	1151..1202	CDD:278556	9/54 (17%)
		RhoGEF	1539..1731	CDD:238091	64/205 (31%)
		PH_RhoGEF	1770..1875	CDD:275411	25/117 (21%)
		DUF2413	2242..>2378	CDD:287302
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	2	1.810