Protein Alignment RhoGEF64C and itsn1

DIOPT Version :10

• Sequence 1: NP_729032.1
• Gene: RhoGEF64C / 38578
• FlyBaseID: FBgn0035574
• Length: 1984
• Species: Drosophila melanogaster
• Sequence 2: XP_005171028.1
• Gene: itsn1 / 368504
• ZFINID: ZDB-GENE-030616-226
• Length: 1751
• Species: Danio rerio
• Alignment Length: 1844
• Identity: 340/1844 - (18%)
• Similarity: 581/1844 - (31%)
• Gaps: 659/1844 - (35%)

  Fly   488 PTSPAIKSILKR-------PTTLELPGVG-------MSPLPLPPVNNSGLVTPAGKSQFYIPTPT 538
            |..|::...:|:       ||...:|.:|       :.|:||||:       |.|.....:..|.
Zfish    94 PLPPSLPPSMKQQPISIPPPTPFGIPSMGAMPGLPAVPPMPLPPL-------PPGVPGMGVSPPL 151

  Fly   539 GTAATPTSSSTPMATSTPTPNASITATTAAA--GAAI--TPG-SRKKVQFMVEDKIIATTATAAT 598
            |.:.||...|  :|...|   |.|...:..:  ||.:  ||. :|..::......:.|.:|.||.
Zfish   152 GASVTPPVPS--LANGAP---AMIQPLSGFSHPGAVLNKTPSFNRSSIKLQKGQSVEAPSAPAAP 211

  Fly   599 TDDSDASAVATKTNY-EYYNR--KRNAATATAAAATAASAAAASPPTAATSTDQQEATLF-AADI 659
            .....|.:.:::..| :.:|.  |..:...|...|......::.|       ..|.||:: .:||
Zfish   212 PPADWAVSQSSRLKYRQLFNSHDKMMSGYLTGPQARTILMQSSLP-------QAQLATIWNLSDI 269

  Fly   660 DDEHNFTT--------------SGEAAPPFIKPDSLHYEDVLPGPQIFDSSESSAKLSLPADGSP 710
            |.:...|.              ||...||.:.||.:        |..|....|.:.:|:.   |.
Zfish   270 DQDGKLTAEEFILAMHLIDMAMSGLPLPPLLPPDLI--------PPTFRRMRSGSGVSVT---SM 323

  Fly   711 HVTTSPVSVLPTGTSNGVGGAGLGPGPAGVSGEEAPATAAATAAATQKTTAANRDYDDDDEHN-- 773
            |.|                         .:..:|.|......|......|..::..::.:..|
Zfish   324 HST-------------------------DLRSQEEPEEEEKEAEKKLPVTFEDKKRENFERGNLE 363

  Fly   774 ----------------------DKDESHDNKPNDL-AEREQDREGDKEQGRAGELGISRQE--KK 813
                                  :|:|....:...| .||.:.:|.||:..|..||...|:|  :|
Zfish   364 LEKRRQALLEQQRKEQERLAALEKEEQERKERERLEQERRRQQELDKQLERQRELERQREEERRK 428

  Fly   814 DAGGKPAASQKRAAQTTQSRNPIRRAHSERHLSTAPLAAHGHASSACAAGRNCHSIPMTPPASGP 878
            :...:.||.::...|........||    :.|.|.               ||...
Zfish   429 EIERREAAKRELERQRQLEWERTRR----QELLTQ---------------RNREQ---------- 464

  Fly   879 QNVIL--NFKDTM-----ALRIKSNRSDSR--DLFQRRPTEPPPPPPPKGAGKGLEPLPHEINKS 934
            :|::|  ..|.|:     ||..|.::.:.:  |:..|                 |....|||..:
Zfish   465 ENIVLLKARKKTLEFELEALNDKKSQLEGKLQDIRSR-----------------LSIQRHEIETT 512

  Fly   935 PLSPQRTVVRVAPFKPQQQQVGGSGDGFT------------------HEVHRLKISHSEEDADLG 981
            ..:.:   :|:|.....|||:..|....:                  :.:||..::..:.    .
Zfish   513 NKTRE---LRIAEITSLQQQLQESQQWLSRLIPDKQCLNDQLKQVQQNSLHRDSLNSLQR----A 570

  Fly   982 VELRRRPNLFLSSPGEEDAAPKKTSILISGDDCYSTM-----------------NLSANAAQPLY 1029
            :|::......|..  :.||..|:|...:...|.::|.                 .|.|:..||.:
Zfish   571 IEMKESTKQQLKE--QLDAVEKETRSKLLEIDAFNTQLKELREIHNKQQRQKQKELEADITQPSH 633

  Fly  1030 QSSVVVNSHSATS-------------------------VCISVSSAEEQ------EQELNQLNTL 1063
            ....:.:..|..|                         ..:|..|.||.      :..|::|.|.
Zfish   634 DRKSIDSQDSRLSGTDDGVSPAWRDDGLGKAPTPPVSQAWMSRVSEEENHSRAEIQDNLSKLFTQ 698

  Fly  1064 RSQ------QMGMGIA--------------------------------------IIPIRG----- 1079
            ..|      |....:|                                      |:.::|
Zfish   699 HPQAGKIQAQSPWSMADKIPVSGFNQEKVKVVYYRAMYPFEARSHDEITIHPGDIVMVKGEWVDE 763

  Fly  1080 -----------------------------ASEVNLQRSVSCISSTS---SSSTSSSSSSSGRGRT 1112
                                         .|||.|....|..||::   .|..||:|||:
Zfish   764 SQTGEPGWLGGEIKGKTGWFPANYAEKIPESEVPLSLRASAASSSAPKLGSHMSSASSST----- 823

  Fly  1113 LIRLDYDRERKGSATSSTPINTPPVISSTPATPPPTDLPIENANPLVPHETELLKI--LRNPVEA 1175
                        :||...|::|.|...:.|::.||...|..:::....:..:....  ..:.||.
Zfish   824 ------------TATPILPVSTEPASIAPPSSAPPPTGPASSSSSASSNWADFSTTWPSNSAVEK 876

  Fly  1176 VKLNLVPHVCGLRA-PTRESGKSKKIQEEEVSGTPVKD-----PGFIAKLLQDPMLGQ--VAEGL 1232
            ...:      |..| ||:.:..|..:.    ||..::.     |..::.....|:|||  ..|||
Zfish   877 QDSD------GWDAWPTQPTQPSLSVP----SGGQIRQRSAFTPATLSGSSPSPVLGQGEKVEGL 931

  Fly  1233 ETETVAELIENSLQRLQQARRGIDMSGTKDHDDM-----------------NRLISASLQR-IRL 1279
            :.:.:..........|...:.  |:....:..||                 .:|||..::: :.:
Zfish   932 QAQALYPWRAKKDNHLNFNKN--DVITVLEQQDMWWFGEVQGQRGWFPKSYVKLISGPVRKSMSI 994

  Fly  1280 E----------RQPATPGISATKE--EDRLSNRGSISSANSFASAHNYEL-----FDFDGG---- 1323
            |          ::| :|.::...|  |.:.||..|:..:..:.:.:.||.     ..|..|
Zfish   995 ESGSSDSPPSVKRP-SPSLNKPTELGEGQNSNSNSMYPSKEYVAMYTYESNEQGDLTFQQGDVIT 1058

  Fly  1324 AGGNESDCYQSCSSELTASGILEEDSVSPIKDPETE-LDAATRAKFYQLLVDATLAEIEHSTHMD 1387
            ....|.|.:..     |.||.:.....:.:|..|:| |.:|.:.
Zfish  1059 VTKKEGDWWTG-----TVSGKIGVFPSNYVKPKESEGLGSAGKT--------------------- 1097

  Fly  1388 QTLGTDSEHHYESIRHSGDPIYEEIHEVPPPL------PLSAPPLNDAELEKKAARSIFEGASKY 1446
            .:||..                .||.:|..|.      .|:..|.....:.||.....:||
Zfish  1098 GSLGKK----------------PEIAQVIAPYTATGAEQLTLAPGQLILIRKKNPGGWWEG---- 1142

  Fly  1447 DILSYLVDAKERGLAREESFDYGNNPKIIEEEAADTLSDLDKRQAEERERDRDSGQSSMSSLSP- 1510
                   :.:.||..|:..:...|..|::....:.|                     :.:..:|
Zfish  1143 -------ELQARGKKRQIGWFPANYVKLLSPSTSKT---------------------TPTDPNPP 1179

  Fly  1511 --PPHNFICGGAGKSSGSDVERQDS-----GVGSETSKTSRSKYQATPTVLHLCEDCD---GAVE 1565
              |..|.:|...|.   .|...|:.     |.|...:..||             ||.|   |.:.
Zfish  1180 KLPTPNAVCQVIGM---YDYTAQNDDELPFGKGQIINVLSR-------------EDPDWWKGELN 1228

  Fly  1566 MQVGDNGILYAPLVC------RKCGKKR-------VERKE--IITEIVETEEKYGRDLQIILEEF 1615
            ..||.....|..|..      :.|....       ||||.  .|.|::.|||.|..|||::.|.|
Zfish  1229 GSVGLFPSNYVKLTTDTDPSQQWCADLHLLDMLTPVERKRQGYIHELIVTEENYVNDLQLVTETF 1293

  Fly  1616 CHPMLVAGLLTQEQLSAIFLNTEDLLENNQTLAERMRDALDMSLEQGD----DDLLTVNIGRIFL 1676
            ..|:|.:.|||:::::.||:|.::|...|..|.:.:|....||.|:..    .|:||..:..
Zfish  1294 QKPLLESDLLTEKEVAMIFVNWKELTMCNIKLLKALRVRKKMSGERMPVKMIGDILTAQLPH--- 1355

  Fly  1677 DFTQMLHAFESYCVRQAGASLLLANLEKEKELLRIFLKVSQMENAVLRRMNLNSFLMVPVQRVTK 1741
                 :..:..:|..|...:.|:.....|....:.|:|...|:.. .:.|.|:|||:.|:||||:
Zfish  1356 -----MQPYIRFCSCQLNGATLIQQKTDEVPEFKDFVKRLAMDPR-CKGMPLSSFLLKPMQRVTR 1414

  Fly  1742 YPLLLARLYKVTPSHLEGRELLKQAQEKIELHLNHINQEAKDVPTKLWRRISSSSPNRRASCEID 1806
            |||::..:.:.||........|:.|.||.|...:.:|:..::
Zfish  1415 YPLIIKNILENTPESHPDHSHLRLALEKAEELCSQVNEGVRE----------------------- 1456

  Fly  1807 MINIKLRKMAIDVLEWNHDEVRFAMEG---RLLYTQPTD----------SNWKKARTIK-----L 1853
                   |...|.|||....|:  .||   :|::...|:          ....||::.|     |
Zfish  1457 -------KENSDRLEWIQAHVQ--CEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFL 1512

  Fly  1854 TPVNALLVTNGKPSANYRAEKAMSEKLNFPKHTGIREASLLLVKEKCGRYTLIREPLYLDRCVVC 1918
            .....||....||..:...:|..|.|.:.                   :|.:.:.|::|:..:|.
Zfish  1513 FNDFLLLTQIIKPLGSSGTDKVFSAKSHL-------------------QYRMYKTPIFLNEVLVK 1558

  Fly  1919 SEAD-----------------------------WDDYFEVQEISSKDTFIFKAEDGVRTKQWYTQ 1954
            ...|                             |     ||:|.:......:.|...|.|.:..:
Zfish  1559 LPTDPSGDEPIFHISHIDRVYTIRAESINERTAW-----VQKIKAASELFIETEKRKREKAYLVR 1618

  Fly  1955 LQYHAQGMGAWRKRRNALANIMIN 1978
            .| .|.|:|          .:|:|
Zfish  1619 SQ-RATGIG----------RLMVN 1631

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF64C	NP_729032.1	RhoGEF	1590..1778	CDD:238091	58/193 (30%)
itsn1	XP_005171028.1	EH	15..112	CDD:197477	3/17 (18%)
		EH	215..293	CDD:197477	14/84 (17%)
		SMC_prok_B	438..>646	CDD:274008	41/262 (16%)
		SH3_Intersectin1_1	731..790	CDD:212920	2/58 (3%)
		INTAP	790..932	CDD:435467	38/168 (23%)
		SH3_Intersectin1_2	932..983	CDD:212922	4/52 (8%)
		SH3	1034..1085	CDD:473055	9/55 (16%)
		SH3_Intersectin1_4	1102..1166	CDD:212926	15/90 (17%)
		SH3_Intersectin1_5	1188..1241	CDD:212928	15/68 (22%)
		RhoGEF	1268..1451	CDD:238091	56/191 (29%)
		PH_13	1470..1612	CDD:465218	26/167 (16%)
		C2_Intersectin	1612..1746	CDD:176021	7/31 (23%)